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  • 1
    Electronic Resource
    Electronic Resource
    Cloning and expression of the Vitreoscilla hemoglobin gene in pseudomonads: effects on cell growth (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 419-424 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The gene (vgb) encoding the hemoglobin (VtHb) of Vitreoscilla sp. was cloned into a broad-host-range vector and stably transformed into Pseudomonas putida, Pseudomonas aeruginosa, and Xanthomonas maltophilia. vgb was stably maintained and expressed in functional form in all three species. When growth of the P. aeruginosa and X. maltophilia transformants in Luria-Bertani medium was compared with that of each corresponding untransformed strain, the VtHb-producing strains reached slightly higher maximum viable cell numbers, had significantly increased viability after extended times in culture, and, like E. coli that produces VtHb, had significantly lower respiration rates. The VtHb-producing strain of P. putida also reached a slightly higher maximum viable cell number than its corresponding untransformed strain, but was significantly less viable after extended times in culture and, unlike the case in E. coli, had a generally higher respiration rate than the untransformed strain. When growth was monitored by absorbance, the results were similar to those obtained with viable cell counts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050576
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence of the region downstream of theVitreoscilla hemoglobin gene:vgb is not part of a multigene operon (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 304-308 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The 1668 base pairs (bp) downstream of theVitreoscilla hemoglobin gene were sequenced in the hope of finding related genes that might be part of an operon. Instead, a sequence was found that constituted an open reading frame (ORF) of 569 amino acids (apparently the carboxy-terminal part of a larger ORF), in the direction opposite to the hemoglobin gene. This sequence was found to have 64% similarity with the 1685 by at the 3′ end of theEscherichia coli uvrA gene. The inferred amino acid sequence of the Vitreoscilla DNA has 69% similarity with the corresponding sequence of theE. coli uvrA protein, with similarities of 90, 100, and 85% in the helix-turn-helix, C-terminal ATP binding, and C-terminal zinc finger domains, respectively. The distance between the 3′ ends of theVitreoscilla hemoglobin anduvrA genes is 63 bp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902733
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and expression of the Vitreoscilla hemoglobin gene in pseudomonads: effects on cell growth (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 419-424 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The gene (vgb) encoding the hemoglobin (VtHb) of Vitreoscilla sp. was cloned into a broad-host-range vector and stably transformed into Pseudomonas putida, Pseudomonas aeruginosa, and Xanthomonas maltophilia. vgb was stably maintained and expressed in functional form in all three species. When growth of the P. aeruginosa and X. maltophilia transformants in Luria-Bertani medium was compared with that of each corresponding untransformed strain, the VtHb-producing strains reached slightly higher maximum viable cell numbers, had significantly increased viability after extebded times in culture, and, like E. coli that produces VtHb, had significantly lower respiration rates. The VtHb-producing strain of P. putida also reached a slightly higher maximum viable cell number than its corresponding untransformed strain, but was significantly less viable after extended times in culture and, unlike the case in E. coli, had a generally higher respiration rate than the untransformed strain. When growth was monitored by absorbance, the results were similar to those obtained with viable cell counts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169938
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  • 4
    Electronic Resource
    Electronic Resource
    Sequence of the region downstream of the Vitreoscilla hemoglobin gene: vgb is not part of a multigene operon (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 304-308 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The 1668 base pairs (bp) downstream of the Vitreoscilla hemoglobin gene were sequenced in the hope of finding related genes that might be part of an operon. Instead, a sequence was found that constituted an open reading frame (ORF) of 569 amino acids (apparently the carboxy-terminal part of a larger ORF), in the direction opposite to the hemoglobin gene. This sequence was found to have 64% similarity with the 1685 bp at the 3′ end of the Escherichia coli uvrA gene. The inferred amino acid sequence of the Vitreoscilla DNA has 69% similarity with the corresponding sequence of the E. coli uvrA protein, with similarities of 90, 100, and 85% in the helix-turn-helix, C-terminal ATP binding, and C-terminal zinc finger domains, respectively. The distance between the 3′ ends of the Vitreoscilla hemoglobin and uvrA genes is 63 bp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902733
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  • 5
    Electronic Resource
    Electronic Resource
    Alterations of the manifestations of hybrid breakdown in Lycopersicon esculentum L. pennellii F2 populations containing L. esculentum versus L. pennellii cytoplasm (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 361-368 
    Details
    ISSN: 1432-2145
    Keywords: Lycopersicon ; Interspecific cross ; Hybrid breakdown ; Fecundity ; Nuclear-cytoplasmic interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reproductive abnormalities reduced the percent stainable pollen, and fruit and seed set in interspecific F2 populations derived from crosses of Lycopersicon esculentum and L. pennellii but were not observed in parental lines and interspecific F1 populations. The degree to which these reproductive abnormalities were expressed in the interspecific F2 populations was affected by cytoplasm. Reproduction was impeded in interspecific F2 populations containing L. esculentum cytoplasm (F 2 Le ) by reduction in pollen production, the lack of fruit set and a high proportion of parthenocarpic fruit among plants capable of fruit set. The F2 populations containing L. pennellii cytoplasm (F 2 Lp4 ) showed a reduced frequency of reproductive abnormalities at all stages of reproductive development, resulting in higher values for percent stainable pollen, fruit and seed set and higher proportions of the F 2 Lp4 populations being capable of setting fruit or seed than F 2 Le populations. The major barrier remaining in F 2 Lp4 populations was reduced fruit set compared to parental lines. The barrier to fruit and seed set observed in the F 2 Le populations, and to a lesser extent in the F 2 Lp4 populations, occurs around the time of fertilization or early embryonic development. The effect of L. pennellii cytoplasm on barriers in the F 2 Lp4 populations is proposed to be due to an interaction between cytoplasmic and nuclear genes during fertilization of the F1 plants to produce F2 populations and may also affect subsequent generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00243204
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  • 6
    Electronic Resource
    Electronic Resource
    Alterations of the manifestations of hybrid breakdown inLycopersicon esculentum L. pennellii F2 populations containingL. esculentum versusL. pennellii cytoplasm (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 125-125 
    Details
    ISSN: 1432-2145
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02153061
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  • 7
    Electronic Resource
    Electronic Resource
    Alterations of the manifestations of hybrid breakdown in Lycopersicon esculentum L. pennellii F2 populations containing L. esculentum versus L. pennellii cytoplasm (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 125-125 
    Details
    ISSN: 1432-2145
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050020
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  • 8
    Electronic Resource
    Electronic Resource
    The coupling effects of some thiol and other sulfur-containing compounds on the circadian rhythm of cell division in photosynthetic mutants of Euglena (1976)
    Springer
    Archives of microbiology 108 (1976), S. 1-8 
    Details
    ISSN: 1432-072X
    Keywords: Euglena ; Mutants ; Circadian rhythm ; Algae ; Biological clock ; Cell division ; Photosynthesis ; Cell cycle ; Thiols ; Synchrony
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous work has demonstrated a persisting, free-running, circadian rhythm of cell division in the P4ZUL photosynthetic mutant of the alga Euglena gracilis Klebs (Strain Z) Pringsheim grown organotrophically in continuous light or darkness at 19° C following prior synchronization by a repetitive LD: 10,14 light cycle. A similar circadian rhythmicity has been recently discovered in the W6ZHL heat-bleached and the Y9ZNalL naladixic acid-induced mutants of Euglena grown under comparable conditions. Over extended timespans, however, these mutants appear to gradually lose first their ability to display persisting overt rhythms, and then even their capability of being entrained by imposed LD cycles. These properties can be restored by the addition of certain sulfur-containing compounds to the medium including cysteine, methionine, dithiothreital, sodium monosulfide, sodium sulfite, and sodium thiosulfate, as well as thioglycolic [mercaptoacetic] acid. The implications of these findings toward biological clock mechanisms are discussed: It appears that some sort of coupling process is operating as opposed to the initiation of an underlying oscillation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425086
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  • 9
    Electronic Resource
    Electronic Resource
    QTL analysis of pest resistance in the wild tomato Lycopersicon pennellii : QTLs controlling acylsugar level and composition (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 709-718 
    Details
    ISSN: 1432-2242
    Keywords: Key words Acylsugars ; Pest resistance ; QTL analysis ; Tomato ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Some accessions of Lycopersicon pennellii, a wild relative of the tomato Lycopersicon esculentum, are resistant to a number of important pests of cultivated tomato due to the accumulation of acylsugars, which constitute 90% of the exudate of type-IV trichomes in L. pennellii LA716. An population, created by the cross L. esculentum×L. pennellii LA 716, was surveyed for acylsugar accumulation and subjected to RFLP/QTL analysis to determine the genomic regions associated with the accumulation of acylglucoses, acylsucroses, and total acylsugars, as well as with acylglucoses as a percentage of total acylsugars (mole percent acylglucoses). Data were analyzed using MAPMAKER/QTL with and without a log10 transformation. A threshold value of 2.4 (default value for MAPMAKER/QTL) was used, as well as 95% empirically derived threshold values. Five genomic regions, two on chromosome 2 and one each on chromosomes 3, 4 and 11, were detected as being associated with one or more aspects of acylsugar production. The L. esculentum allele is partially dominant to the L. pennellii allele in the regions on chromosomes 2 and 11, but the L. pennellii allele is dominant in the region on chromosome 3. Throughout this study, we report the comparative effects of analytical methodology on the identification of acylsugar QTLs. Similarities between our results and published results for the genus Solanum are also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050183
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  • 10
    Electronic Resource
    Electronic Resource
    QTL analysis of pest resistance in the wild tomato Lycopersicon pennellii: QTLs controlling acylsugar level and composition (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 709-718 
    Details
    ISSN: 1432-2242
    Keywords: Acylsugars ; Pest resistance ; QTL analysis ; Tomato ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Some accessions of Lycopersicon pennellii, a wild relative of the tomato Lycopersicon esculentum, are resistant to a number of important pests of cultivated tomato due to the accumulation of acylsugars, which constitute 90% of the exudate of type-IV trichomes in L. pennellii LA716. An interspecific F2 population, created by the cross L. esculentum x L. pennellii LA 716, was surveyed for acylsugar accumulation and subjected to RFLP/QTL analysis to determine the genomic regions associated with the accumulation of acylglucoses, acylsucroses, and total acylsugars, as well as with acylglucoses as a percentage of total acylsugars (mole percent acylglucoses). Data were analyzed using MAPMAKER/QTL with and without a log10 transformation. A threshold value of 2.4 (default value for MAPMAKER/QTL) was used, as well as 95% empirically derived threshold values. Five genomic regions, two on chromosome 2 and one each on chromosomes 3, 4 and 11, were detected as being associated with one or more aspects of acylsugar production. The L. esculentum allele is partially dominant to the L. pennellii allele in the regions on chromosomes 2 and 11, but the L. pennellii allele is dominant in the region on chromosome 3. Throughout this study, we report the comparative effects of analytical methodology on the identification of acylsugar QTLs. Similarities between our results and published results for the genus Solanum are also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226093
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