ALBERT

Notes: Abstract Based on somatic cell genetic analysis, autosomal gene linkage is reported for the supernatant enzymes of human isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH) in human-mouse cell hybrids. The IDH, MDH linkage was not linked to the X and E 17 chromosomes or to 12 additional human enzyme markers.

Notes: Abstract To elucidate the mechanisms involved in the regulation of uricase activity in Drosophila melanogaster, a comparative analysis of the patterns of uricase activity during development was undertaken for the wild type, Ore-R, and the mutants ry 2 and ma-1. Uricase activity in ry 2 and ma-l, unlike that in Ore-R, increased rapidly following emergence of the adult. This study indicates that uricase in Drosophila, in contrast to that in several microorganisms, is not induced by uric acid, since ry 2 and ma-l with no detectable uric acid have higher activity than the wild type.

Notes: Abstract There is an implicit assumption in most policy studies that once a policy has been formulated the policy will be implemented. This assumption is invalid for policies formulated in many Third World nations and for types of policies in Western societies. Third World governments tend to formulate broad, sweeping policies, and governmental bureaucracies often lack the capacity for implementation. Interest groups, opposition parties, and affected individuals and groups often attempt to influence the implementation of policy rather than the formulation of policy. A model of the policy implementation process is presented. Policy implementation is seen as a tension generating force in society. Tensions are generated between and within four components of the implementing process: idealized policy, implementing organization, target group, and environmental factors. The tensions result in transaction patterns which may or may not match the expectations of outcome of the policy formulators. The transaction patterns may become crystallized into institutions. Both the transaction patterns and the institutions may generate tensions which, by feedback to the policymakers and implementors, may support or reject further implementation of the policy. By application of the model, policymakers can attempt to minimize disruptive tensions which can result in the failure of policy outcomes to match policy expectations.

Notes: Abstract This paper presents an empirical analysis of individuals in policy formulating and policy implementing roles. Data for the comparison are from interviews with a random sample of 119 New Zealand middle-level public administrators from 27 government departments. The individuals in the policy formulating roles were slightly younger; however, those in the formulating and implementing roles varied little from each other in terms of social background, educational attainment and career patterns. Few differences were evident in regard to job satisfaction, decisional authority, and hierarchical relations. Significant differences between policy formulators and policy implementors were discovered in terms of work load, career aspirations, and awareness of political influences in governmental policymaking. From the evidence of this study, the New Zealand administrative system does not allocate policy formulating roles to individuals different from those who implement policies. Differences between formulators and implementors in the New Zealand system appear to stem from the nature of the work of the two policy roles.

Notes: Abstract The interpretation of diagnostic parameters in the spectral reflectance data for asteroids provides a means of characterizing the mineralogy and petrology of asteroid surface materials. An interpretive technique based on a quantitative understanding of the functional relationship between the optical properties of a mineral assemblage and its mineralogy, petrology and chemistry can provide a considerably more sophisticated characterization of a surface material than any matching or classification technique for those objects bright enough to allow spectral reflectance measurements. Albedos derived from radiometry and polarization data for individual asteroids can be used with spectral data to establish the spectral albedo, to define the optical density of the surface material and, in general, to constrain mineralogical interpretations. Mineral assemblages analogous to most meteorite types, with the exception of ordinary chondritic assemblages, have been found as surface materials of Main Belt asteroids. C1- and C2-like assemblages (unleached, oxidized meteoritic clay minerals plus opaques such as carbon) dominate the population (∼80%) throughout the Belt, especially in the outer Belt. A smaller population of asteroids exhibit surface materials similar to C3 (CO, CV) meteoritic assemblages (olivine plus opaque, probably carbon) and are also distributed throughout the Belt. The relative size (diameter) distributions for these two populations of objects are consistent with an origin by sequential accretion from a cooling nebula (‘C2’ as surface layers, ‘C3’ as interior layers or cores). Based on information from meteoritic analogues and on qualitative models for the behavior of these materials during a heating episode, it seems unlikely that these ‘C2’- and ‘C3’-like asteroidal bodies have experienced any significant post-accretionary heating event either near surface or in the deep interior. The majority of remaining studied asteroids (20) of 65 asteroids exhibit spectral reflectance curves dominated by the presence of metallic nickel-iron in their surface materials. These objects are most probably the several end products of an intense thermal event leading to the melting and differentiating of their protobodies. These thermalized bodies are concentrated toward the inner part of the Asteroid Belt but exist throughout the Belt. The size of the proto-asteroid has apparently exercised control over the post-accretionary thermal history of these bodies. The available evidence indicates that all asteroids larger than about 450 km in (present) diameter have undergone a significant heating episode since their formation. The post-accretionary thermal history of the asteroidal parent bodies was apparently affected by both distance from the Sun and body size. The C2-like materials which dominate the main asteroid belt population appear to be relatively rare on earth-approaching asteroids. This suggests that most of these Apollo-Amor objects are not randomly derived from the main belt, but (a) may derive from a single event in recent time (∼107 yr), (b) may derive from a favorably situated source body, (c) may derive from a particular, compositionally anomalous region of the belt, or (d) may derive from an alternate source (e.g. comets).

Notes: Abstract Using somatic cell hybridization gene mapping methodology, genes coding for human cell-surface proteins have been assigned to specific chromosomes. Lactoperoxidase-catalyzed iodination was employed to label external membrane proteins in cell hybrids between mouse and human cultured cells. Mouse and human external membrane proteins were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis. After electrophoresis, external membrane proteins were identified by autoradiography. An external membrane protein of 130,000 molecular weight (EMP-130) segregated concordantly with glutamic oxaloacetic transaminases (GOTs, EC 2.6.1.1), an enzyme marker encoded on chromosome 10. External membrane proteins of 195,000 and 175,000 molecular weight (EMP-195 and EMP-175) segregated concordantly with nucleoside phosphorylase (NP, EC 2.4.2.1), an enzyme marker encoded on chromosome 14. Limited proteolysis of the 195,000 and 175,000 molecular weight proteins suggests that these two proteins are modified forms of each other and are encoded by the same locus. These findings demonstrate the mapping of human genes coding for external proteins EMP-130 and EMP-195 to chromosomes 10 and 14, respectively. Chromosome analyses of cell hybrids supported these assignments.

Notes: Abstract The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400–6000 was found to occur in the concentration range of 50–55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.

Notes: Abstract A bioautographic assay was developed for the visualization of aminoacylase-1 (N -acylamino acid aminohydrolase, ACY-1; EC 3.5.1.14) after zone electrophoresis. Bioautography and species differences in electrophoretic mobility of ACY-1 made it possible to investigate the chromosome assignment of the gene for human ACY-1 using human—mouse somatic cell hybrids. Human ACY-1 segregated concordantly with β-galactosidase-A (βGAL A;EC 3.2.1.23) but showed discordant segregation with 32 other markers representing 23 linkage groups. The β GALA gene has been previously assigned to chromosome 3. From this evidence and confirming chromosome analyses, ACY-1has been assigned to chromosome 3. A genetic polymorphism in the electrophoretic mobility of ACY was observed in mouse strains, demonstrating that this enzyme can be mapped in genetic crosses of Mus musculus.

Notes: Abstract The structural gene (βGALA) coding for lysosomal β-galactosidase- A (EC 3.2.1.23) has been assigned to human chromosome 3 using man-mouse somatic cell hybrids. Human β-galactosidase-A was identified in cell hybrids with a species-specific antiserum to human liver β-galactosidase-A. The antiserum precipitates β-galactosidase-A from human tissues, cultured cells, and cell hybrids, and recognizes cross-reacting material from a patient with GM1 gangliosidosis. We have analyzed 90 primary man-mouse hybrids derived from 12 separate fusion experiments utilizing cells from 9 individuals. Enzyme segregation analysis excluded all chromosomes for βGALA assignment except chromosome 3. Concordant segregation of chromosomes and enzymes in 16 cell hybrids demonstrated assignment of βGALA to chromosome 3; all other chromosomes were excluded. The evidence suggests that GM1 gangliosidosis is a consequence of mutation at this βGALA locus on chromosome 3.