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  • Phaseolus  (24)
  • Pisum  (23)
  • Springer  (46)
  • Periodicals Archive Online (PAO)
  • 2010-2014
  • 1980-1984  (26)
  • 1975-1979  (20)
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  • Springer  (46)
  • Periodicals Archive Online (PAO)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Identification by combined gas chromatography-mass spectrometry of phaseic acid and dihydrophaseic acid and characterization of further abscisic acid metabolites in pea seedlings (1979)
    Springer
    Planta 147 (1979), S. 168-173 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid metabolites ; Dihydrophaseic acid ; Phaseic acid ; Pisum ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seven day old seedlings of Pisum sativum L., cv. Kleine Rheinländerin, were wilted for 3 days. After partially removing the roots, they were rewatered and at the same time radioactive abscisic acid([1-14C]ABA, spec. activity 1.7·108d s-1mmol-1) was applied for 1 h via the xylem of the roots. After 24 h, 4 days, and 12 days the seedlings were extracted and the metabolites of ABA were analyzed by means of thin-layer and gas chromatography in combination with mass spectrometry, autoradiography, and scintillation counting. Phaseic acid (PA) and dihydrophaseic acid (DPA) were identified as metabolites of ABA. The presence of another ABA-metabolite was also demonstrated. From its mass spectrum it has been postulated that this metabolite is 4′-desoxy-ABA. In addition to these substances, several other metabolites, which are more polar than ABA and its known degradation products, were present in the seedlings. The quantity and number of these unknown metabolites increased with time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389520
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  • 2
    Electronic Resource
    Electronic Resource
    Role of ATP in nitrite reduction in roots of wheat and pea (1981)
    Springer
    Planta 152 (1981), S. 234-238 
    Details
    ISSN: 1432-2048
    Keywords: ATP and nitrite reduction ; Glucose-6-phosphate ; Nitrite reduction ; Pisum ; Plastids, nitrite reduction ; Root, plastids ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Excised wheat (Triticum aestivum L.) and field pea (Pisum arvense L.) roots, incubated under anaerobic conditions or in the presence of uncouplers of oxidative phosphorylation [2,4-dinitrophenol (DNP), carbonylcyanide-m-chlorophenylhydrazone, pentachlorophenol] accumulated nitrite as a result of an inhibition of nitrite reduction. In isolated root plastids, nitrite reduction was dependent on a supply of glucose-6-phosphate (G6P) and did not require ATP. The estimated Km value for glucose 6-phosphate was 1.25 mM. Glucose and fructose-1,6-diphosphate were ineffective substrates for nitrate reduction. Anaerobic conditions and treatment with DNP, which would result in a cessation of ATP production by the mitochondria and a stimulation of glycolysis via the “Pasteur effect”, were shown to decrease the G6P content of excised roots of wheat and pea. A negative correlation was observed between the level of G6P and nitrite accumulation on root tissues. It is proposed that an interruption in the supply of G6P to the root plastid under these conditions would result in an inhibition of nitrite reduction leading to nitrite accumulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385149
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  • 3
    Electronic Resource
    Electronic Resource
    Changing cell-wall compositions in hypocotyls of dark-grown bean seedlings (1980)
    Springer
    Planta 149 (1980), S. 209-212 
    Details
    ISSN: 1432-2048
    Keywords: Cell elongation ; Cell wall ; Glucan ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyls of dark-grown 6-day-old seedlings of Phaseolus vulgaris L. proved to be sufficiently homogeneous to permit studies relating the rate of cell elongation to the composition of the primary cell walls. Whereas the levels of cellulose and uronic acids remained practically constant during and after cell extension, all other components showed major or minor changes. Cell-wall protein, as such, decreased by more than 50%, but indications are that hydroxyproline-rich glycoprotein increased with a decreasing rate of cell elongation, concomitant with a rise in the degree of arabinosylation of wall-bound hydroxyproline. As cell elongation slowed down, non-cellulosic glucose accumulated, presumably in the form of a β-(1–4)glucan closely associated with cellulose. These findings confirm the notion that the primary cell wall is a highly dynamic structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384555
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  • 4
    Electronic Resource
    Electronic Resource
    Cell length, light and14C-labelled indol-3yl-acetic acid transport inPisum satisum L. andPhaseolus vulgaris L (1980)
    Springer
    Planta 149 (1980), S. 327-331 
    Details
    ISSN: 1432-2048
    Keywords: Auxin transport ; Cell length ; Light and auxin transport ; Phaseolus ; Pisum ; Transport (auxin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The putative auxin-transporting cells of the intact herbaceous dicotyledon are the young, differentiating vascular elements. The length of these cells was found to be considerably greater in dwarf (Meteor) than in tall (Alderman) varieties ofPisum sativum L., and to be greater in etiolated than in light-grown plants ofP. sativum cv Meteor andPhaseolus vulgaris L. cv Mexican Black. Under given light conditions during transport these large differences in cell length did not influence the shapes of the transport profiles or the velocity of transport of14C-labelled indol-3yl-acetic acid (IAA) applied to the apical bud. However, in both etiolated and light-grown bean and dwarf pea plants the velocity of transport in darkness was ca. 25% lower than that in light. Under the same conditions of transport velocities in bean were about twice those observed in the dwarf pea. Exposure to light during transport increased the rate of export of14C from the labelled shoot apex in green dwarf pea plants but not in etiolated plants. The light conditions to which the plants were exposed during growth and transport had little effect on the rates of uptake of IAA from the applied solutions. The results indicate that the velocity of auxin transport is independent of the frequency of cell-to-cell interfaces along the transport pathway and it is suggested that in intact plants auxin transport is entirely symplastic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571165
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  • 5
    Electronic Resource
    Electronic Resource
    Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves (1981)
    Springer
    Planta 153 (1981), S. 376-387 
    Details
    ISSN: 1432-2048
    Keywords: CO2 assimilation ; Electron transport ; Gas exchange ; Phaseolus ; Photosynthesis (C3) ; Ribulose bisphosphate carboxylase-oxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A series of experiments is presented investigating short term and long term changes of the nature of the response of rate of CO2 assimilation to intercellular p(CO2). The relationships between CO2 assimilation rate and biochemical components of leaf photosynthesis, such as ribulose-bisphosphate (RuP2) carboxylase-oxygenase activity and electron transport capacity are examined and related to current theory of CO2 assimilation in leaves of C3 species. It was found that the response of the rate of CO2 assimilation to irradiance, partial pressure of O2, p(O2), and temperature was different at low and high intercellular p(CO2), suggesting that CO2 assimilation rate is governed by different processes at low and high intercellular p(CO2). In longer term changes in CO2 assimilation rate, induced by different growth conditions, the initial slope of the response of CO2 assimilation rate to intercellular p(CO2) could be correlated to in vitro measurements of RuP2 carboxylase activity. Also, CO2 assimilation rate at high p(CO2) could be correlated to in vitro measurements of electron transport rate. These results are consistent with the hypothesis that CO2 assimilation rate is limited by the RuP2 saturated rate of the RuP2 carboxylase-oxygenase at low intercellular p(CO2) and by the rate allowed by RuP2 regeneration capacity at high intercellular p(CO2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384257
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  • 6
    Electronic Resource
    Electronic Resource
    A study of the subcellular localisation of an ethylene binding site in developing cotyledons of Phaseolus vulgaris L. by high resolution autoradiography (1982)
    Springer
    Planta 154 (1982), S. 48-52 
    Details
    ISSN: 1432-2048
    Keywords: Cotyledon ; Endoplasmic reticulum ; Ethylene binding site ; Phaseolus ; Protein body membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Use was made of light microscopy and high resolution electron microscope autoradiography to determine the subcellular localisation of a binding site with a high affinity and specificity for ethylene in developing cotyledons of Phaseolus vulgaris L. The results indicate that the binding site is located on the endoplasmic reticulum and protein body membranes, confirming previous studies using cellular fractionation and marker enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385495
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  • 7
    Electronic Resource
    Electronic Resource
    Transport of exogenous auxin in two-branched dwarf pea seedlings (Pisum sativum L.) (1977)
    Springer
    Planta 136 (1977), S. 91-96 
    Details
    ISSN: 1432-2048
    Keywords: Apical dominance ; Auxin transport ; Cambial development ; Pisum ; Polarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dwarf pea plants bearing two cotyledonary shoots were obtained by removing the epicotyl shortly after germination, and the patterns of distribution of 14C in these plants was investigated following the application of [14C]IAA to the apex of one shoot. Basipetal transport to the root system occurred, but in none of the experiments was 14C ever detected in the unlabelled shoot even after transport periods of up to 48 h. This was true both of plants with two equal growing shoots and of plants in which one shoot had become correlatively inhibited by the other, and in the latter case applied whether the dominant or subordinate shoot was labelled. In contrast, when [14C]IAA was applied to a mature foliage leaf of one shoot transfer of 14C to the other shoot took place, although the amount transported was always low. Transport of 14C from the apex of a subordinate shoot on plants bearing one growing and one inhibited shoot was severely restricted compared with the transport from the dominant shoot apex, and in some individual plants no transport at all was detected. Removal of the dominant shoot apex rapidly restored the capacity of the subordinate shoot to transport apically-applied [14C]IAA, and at the same time led to rapid cambial development and secondary vascular differentiation in the previously inhibited shoot. Applications of 1% unlabelled IAA in lanolin to the decapitated dominant shoot maintained the inhibition of cambial development in the subordinate shoot and its reduced capacity for auxin transport. These results are discussed in relation to the polarity of auxin transport in intact plants and the mechanism of correlative inhibition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387930
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  • 8
    Electronic Resource
    Electronic Resource
    Influence of winter and summer growth conditions on leaf membrane lipids of Pisum sativum L. (1983)
    Springer
    Planta 157 (1983), S. 218-223 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast membranes ; Fatty-acid composition (leaf membranes) ; Pisum ; Seasonal variation (fatty acids) ; Thylakoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipid composition and degree of unsaturation of fatty acids has been measured for membranes isolated from leaves of Pisum sativum grown under either summer or winter conditions. Although the lipid-class composition was not significantly changed for the two growth conditions the degree of unsaturation of the fatty acids was greater for winter than summer plants. The difference in unsaturation was evident with all lipid classes of the non-thylakoid membranes including the galactolipids of the chloroplast envelope. In contrast, both the relative amounts of lipid classes and degree of saturation were not greatly changed for summer and winter thylakoids with the exception that phosphatidylglycerol had a greater linolenic acid (18:3) content for the thylakoids of winter grown leaves. However, a striking difference was found for the total acyl lipid to chlorophyll ratio for thylakoids isolated from summer or winter plants, with the former producing a lower ratio than the latter growth conditions. The above changes in lipid composition of chloroplast membranes are discussed in terms of optimizing their functional activities under the different growth conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405185
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  • 9
    Electronic Resource
    Electronic Resource
    Stomatal responses of Argenteum — a mutant of Pisum sativum L. with readily detachable leaf epidermis (1982)
    Springer
    Planta 155 (1982), S. 146-153 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Cytokinin ; Mutant (barley) ; Pisum ; Potassium and stomata protoplast ; Stomata (epidermis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epidermis is easily detached from both adaxial and abaxial surfaces of leaf four of the Argenteum mutant of Pisum sativum L. The isolated epidermis has stomata with large, easily-measured pores. Hairs and glands are absent. The density of stomata is high and contamination by mesophyll cells is low. In the light and in CO2-free air, stomata in isolated adaxial epidermis of Argenteum mutant opened maximally after 4 h incubation at 25°C. The response of stomata to light was dependent on the concentration of KCl in the incubation medium and was maximal at 50 mol m-3 KCl. Stomata did not respond to exogenous kinetin, but apertures were reduced by incubation of epidermis on solutions containing between 10-5 and 10-1 mol m-3 abscisic acid (ABA). The responses of stomata of Argenteum mutant to light, exogenous KCl, ABA and kinetin were comparable with those described previously for stomata in isolated epidermis of Commelina communis. A method for preparing viable protoplasts of guard cells from isolated epidermis of Argenteum mutant is described. The response of guard cell protoplasts to light, exogenous KCl, ABA and kinetin were similar to those of stomata in isolated epidermis except that the increase in volume of the protoplasts in response to light was maximal at a lower concentration of KCl (10 mol m-3) and that protoplasts responded more rapidly to light than stomata in isolated epidermis. The protoplasts did not respond to exogenous kinetin, but when incubated for 1 h in the light and in CO2-free air on a solution containing 10-3 mol m-3 ABA, they decreased in volume by 30%. The advantages of using epidermis from Argenteum mutant for experiments on stomatal movements are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392545
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  • 10
    Electronic Resource
    Electronic Resource
    In-vitro translation of messenger-RNA from developing bean leaves. Evidence for the existence of stored messenger-RNA and its light-induced mobilisation into polyribosomes (1977)
    Springer
    Planta 136 (1977), S. 31-36 
    Details
    ISSN: 1432-2048
    Keywords: Phaseolus ; Photo-induction ; Polyribosomes ; mRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Poly(A)-containing messenger RNA was purified from polyribosomes isolated from the primary leaves of 7-day-old dark-grown seedlings of Phaseolus vulgaris var. Masterpiece. Analysis of the messenger RNA on 2.4% polyacrylamide gels showed that it consists of a heterogeneous population of molecules with an average molecular weight of 500,000. The nucleotide composition of the RNA was 16.0% cytidylic acid, 39.4% adenylic acid, 21.3% guanylic acid and 23.2% uridylic acid. Based on the degree of resistance of the RNA to digestion with ribonucleases A and T1 the average length of the poly(A) sequence was calculated to be 120 nucleotides. No significant differences in mobility in polyacrylamide gels, nucleotide composition or polyadenylic acid content were found between the poly(A)-containing mRNA from polyribosomes of primary leaves of dark-grown plants and those given a 16 h white light treatment. Purified poly(A)-containing mRNA was shown to direct the incorporation of [35S]methionine into proteins in an in vitro protein-synthesising system from wheat germ. The protein products were fractionated according to molecular size by electrophoresis in 15% polyacrylamide/urea/SDS gels and the protein bands were detected by fluorography. Messenger RNAs directing the synthesis of three polypeptides with molecular weights of 34,000, 32,000 and 25,000 were detected in polyribosomes of plants following white light treatment. These messenger RNAs were absent, or present in much lower amounts, in polyribosomal messenger RNA from leaves of dark-grown plants, although they were present in total cell poly(A)-containing RNA. This indicates that certain messenger RNAs may be stored in the dark and that light stimulates these RNAs to engage in polyribosome formation. Continuous far-red (730 nm) irradiation for 4 h also caused the appearance of these messenger RNAs in the polyribosomes although 5 min red light followed by 4 h darkness had little effect. This suggests that phytochrome acting in the “high energy” mode, may be the photoreceptor responsible for initiating the response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387921
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