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  • 2015-2019  (430)
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  • 1
    Call number: 15/S 02.0237 ; 15/S 02.0237(22) 2. Ex.
    In: Geotechnologien science report
    Description / Table of Contents: Joint Research Project CO2MAN (CO2MAN Reservoir Management): Continuation of Research and Development Work for CO2 Storage at the Ketzin Pilot SiteMONACO - Monitoring Approach for Geological CO2 Storage Sites Using a Hierarchical Observation Concept -- Advances in Stable Isotope Monitoring of Injected CO2 Under Elevated Pressures, Temperatures and Salinities: Selected Results from the Project CO2ISO-LABEL -- CO2BioPerm - Influence of Bio-Geochemical CO2-Transforrmation Processes on the Long-Term Permeability -- Seismic and Sub-seismic Deformation Prediction in the Context of Geological Carbon Trapping and Storage -- Long-Term Safety of Well Abandonment: First Results from Large Scale Laboratory Experiments (COBRA) -- "CO2RINA" - CO2 storage Risk Integrated Analysis -- Saltwater Monitoring Using Long-Electrode ERT -- Carbon Dioxide Storage in Eastern Brandenburg: Implications for Synergetic Geothermal Heat Recovery and Conceptualization of an Early Warning System Against Freshwater Salinization - BRINE -- Combined Natural and Social Science Approach for Regional-Scale Characterisation of CO2 Storage Formations and Brine Migration Risks (CO2Brim) -- Chances for and Limitations of Acceptance for CCS in Germany.
    Description / Table of Contents: This book explores the industrial use of secure, permanent storage technologies for carbon dioxide (CO2), especially geological CO2 storage. Readers are invited to discover how this greenhouse gas could be spared from permanent release into the atmosphere through storage in deep rock formations. Themes explored here include CO2 reservoir management, caprock formation, bio-chemical processes and fluid migration. Particular attention is given to groundwater protection, the improvement of sensor technology, borehole seals and cement quality. A collaborative work by scientists and industrial partners, this volume presents original research, it investigates several aspects of innovative technologies for medium-term use and it includes a detailed risk analysis. Coal-based power generation, energy consuming industrial processes (such as steel and cement) and the burning of biomass all result in carbon dioxide. Those involved in such industries who are considering geological storage of CO2, as well as earth scientists and engineers will value this book and the innovative monitoring methods described. Researchers in the field of computer imaging and pattern recognition will also find something of interest in these chapters.
    Type of Medium: Series available for loan
    Pages: X, 245 S. : Ill., graph. Darst., Kt.
    ISBN: 9783319139296
    Series Statement: Geotechnologien science report 22
    Classification:
    Geophysical Deep Sounding
    Location: Reading room
    Location: Reading room
    Branch Library: GFZ Library
    Branch Library: GFZ Library
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  • 2
    Monograph available for loan
    Monograph available for loan
    Wiesbaden : Springer VS
    Call number: IASS 16.90508
    Type of Medium: Monograph available for loan
    Pages: X, 169 Seiten
    ISBN: 3658037563 (pbk) , 9783658037567 (pbk) , 9783658037574 (electronic)
    Series Statement: Lehrbuch
    Language: German
    Branch Library: RIFS Library
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  • 3
    Call number: AWI G6-18-91956
    Description / Table of Contents: Earth's climate varies continuously across space and time, but humankind has witnessed only a small snapshot of its entire history, and instrumentally documented it for a mere 200 years. Our knowledge of past climate changes is therefore almost exclusively based on indirect proxy data, i.e. on indicators which are sensitive to changes in climatic variables and stored in environmental archives. Extracting the data from these archives allows retrieval of the information from earlier times. Obtaining accurate proxy information is a key means to test model predictions of the past climate, and only after such validation can the models be used to reliably forecast future changes in our warming world. The polar ice sheets of Greenland and Antarctica are one major climate archive, which record information about local air temperatures by means of the isotopic composition of the water molecules embedded in the ice. However, this temperature proxy is, as any indirect climate data, not a perfect recorder of past climatic variations. Apart from local air temperatures, a multitude of other processes affect the mean and variability of the isotopic data, which hinders their direct interpretation in terms of climate variations. This applies especially to regions with little annual accumulation of snow, such as the Antarctic Plateau. While these areas in principle allow for the extraction of isotope records reaching far back in time, a strong corruption of the temperature signal originally encoded in the isotopic data of the snow is expected. This dissertation uses observational isotope data from Antarctica, focussing especially on the East Antarctic low-accumulation area around the Kohnen Station ice-core drilling site, together with statistical and physical methods, to improve our understanding of the spatial and temporal isotope variability across different scales, and thus to enhance the applicability of the proxy for estimating past temperature variability. The presented results lead to a quantitative explanation of the local-scale (1–500 m) spatial variability in the form of a statistical noise model, and reveal the main source of the temporal variability to be the mixture of a climatic seasonal cycle in temperature and the effect of diffusional smoothing acting on temporally uncorrelated noise. These findings put significant limits on the representativity of single isotope records in terms of local air temperature, and impact the interpretation of apparent cyclicalities in the records. Furthermore, to extend the analyses to larger scales, the timescale-dependency of observed Holocene isotope variability is studied. This offers a deeper understanding of the nature of the variations, and is crucial for unravelling the embedded true temperature variability over a wide range of timescales.
    Type of Medium: Dissertations
    Pages: xxi, 197 Seiten , Illustrationen, Diagramme
    Language: English
    Note: Contents: 1 General introduction. - 1.1 Challenges of isotope-based temperature reconstructions. - 1.2 Thesis overview. - 1.3 Author contributions. - 2 Theoretical background. - 2.1 The isotopic composition of firn and ice. - 2.1.1 Fractionation of water isotopologues. - 2.1.2 Relationship with temperature. - 2.1.3 Measuring of the isotopic composition. - 2.2 Processes within the firn column. - 2.2.1 The firn column of polar ice sheets. - 2.2.2 The density of firn. - 2.2.3 The temperature profile of firn. - 2.2.4 Vapour diffusion in firn. - 2.3 Internal climate variability. - 3 Regional climate signal vs.local noise: a two-dimensional view of water isotopes. - 3.1 Introduction. - 3.2 Data and methods. - 3.3 Results. - 3.3.1 Trench isotope records. - 3.3.2 Single-profile representativity. - 3.3.3 Mean trench profiles. - 3.3.4 Spatial correlation structure. - 3.3.5 Statistical noise model. - 3.4 Discussion. - 3.4.1 Local noise vs. regional climate signal. - 3.4.2 Representativity of isotope signals. - 3.4.3 Implications. - 3.5 Conclusions. - 3.6 Appendix A: Derivation of noise model. - 3.6.1 Definitions. - 3.6.2 Derivation of model correlations. - 3.6.3 Estimation of parameters. - 3.7 Appendix B: Noise level after diffusion. - 4 Constraints on post-depositional isotope modifications in east antarctic firn. - 4.1 Introduction. - 4.2 Data and methods. - 4.2.1 Sampling and measurements. - 4.2.2 Trench depth scale. - 4.2.3 Spatial variability of trench profiles. - 4.2.4 Quantification of downward advection, densification and diffusion. - 4.2.5 Statistical tests. - 4.3 Results. - 4.3.1 Comparison of T15 and T13 isotope data. - 4.3.2 Expected isotope profile changes. - 4.3.3 Temporal vs. spatial variability. - 4.4 Discussion. - 4.4.1 Densification, diffusion and stratigraphic noise. - 4.4.2 Additional post-depositional modifications. - 4.5 Conclusions. - 5 On the similarity and apparent cycles of isotope variations. - 5.1 Introduction. - 5.2 Data and Methods. - 5.2.1 Data. - 5.2.2 Spectral analysis. - 5.2.3 Rice’s formula. - 5.2.4 Cycle length and amplitude estimation. - 5.2.5 Model for vertical isotope profiles. - 5.3 Results. - 5.3.1 Spectral analysis of isotope profiles. - 5.3.2 Theoretical and observed cycle length. - 5.3.3 Illustrative examples. - 5.3.4 Depth dependency of cycle length. - 5.3.5 Simulated vs. observed isotope variations. - 5.4 Discussion and summary. - 5.5 Conclusions. - 5.6 Appendix A: Input sensitivity. - 5.7 Appendix B: Additional results. - 5.8 Appendix C: Spectral significance testing. - 6 Timescale-dependency of antarctic isotope variations. - 6.1 Introduction. - 6.2 Data and methods. - 6.2.1 DML and WAIS isotope records. - 6.2.2 Spectral model. - 6.2.3 Timescale-dependent signal-to-noise ratio. - 6.2.4 Effects of diffusion and time uncertainty. - 6.2.5 Present-day temperature decorrelation. - 6.3 Results. - 6.3.1 Illustration of model approach. - 6.3.2 DML and WAIS isotope variability. - 6.4 Discussion. - 6.4.1 Interpretation of noise spectra. - 6.4.2 Interpretation of signal spectra. - 6.4.3 Signal-to-noise ratios. - 6.4.4 Differences between DML and WAIS. - 6.5 Conclusions. - 7 Declining temperature variability from LGM to holocene. - 8 General discussion and conclusions. - 8.1 Short-scale spatial and temporal isotope variability. - 8.1.1 Local spatial variability. - 8.1.2 Seasonal to interannual variability. - 8.1.3 Spatial vs. temporal variability. - 8.2 Extension to longer scales. - 8.2.1 Spatial vs. temporal variability on interannual timescales. - 8.2.2 Holocene and longer timescales. - 8.3 Concluding remarks and outlook. - Bibliography. - A Methods to: declining temperature variability from lgm to holocene. - A.1 Temperature proxy data. - A.2 Model-based temperature and variability change. - A.3 Temperature recalibration of proxy records. - A.3.1 Recalibration of ice-core records. - A.3.2 Recalibration of marine records. - A.4 Variance and variance ratio estimation. - A.5 Noise correction. - A.5.1 Testing effect of noise correction. - A.6 Effect of ecological adaption and bioturbation. - A.7 Effect of proxy sampling locations. - B Layering of surface snow and firn: noise or seasonal signal?. - B.1 Introduction. - B.2 Materials and methods. - B.2.1 Firn-core density profiles. - B.2.2 Trench density profiles. - B.2.3 Dielectric profiling and density estimates. - B.2.4 Comparison of DEP and CT density. - B.2.5 Ion measurements. - B.3 Results. - B.3.1 2-D trench density data. - B.3.2 Spatial correlation structure. - B.3.3 Comparison of mean density, isotope and impurity profiles. - B.3.4 Spectral analysis of vertical density data. - B.4 Discussion. - B.4.1 Spatial variability. - B.4.2 Representativeness of single profiles. - B.4.3 Seasonal cycle in snow density. - B.4.4 Density layering in firn and impurities. - B.5 Conclusions. - Acknowledgements - Danksagung.
    Location: AWI Reading room
    Branch Library: AWI Library
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  • 4
    Publication Date: 2019-03-05
    Print ISSN: 1553-734X
    Electronic ISSN: 1553-7358
    Topics: Biology , Computer Science
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  • 5
    Publication Date: 2016-09-06
    Description: Hereditary persistence of fetal hemoglobin (HPFH) is a condition in some individuals who have a high level of fetal hemoglobin throughout life. Individuals with compound heterozygous β-thalassemia or sickle cell disease (SCD) and HPFH have milder clinical manifestations. Using RNA-guided clustered regularly interspaced short palindromic repeats-associated Cas9 (CRISPR-Cas9) genome-editing technology, we deleted, in normal hematopoietic stem and progenitor cells (HSPCs), 13 kb of the β-globin locus to mimic the naturally occurring Sicilian HPFH mutation. The efficiency of targeting deletion reached 31% in cells with the delivery of both upstream and downstream breakpoint guide RNA (gRNA)-guided Staphylococcus aureus Cas9 nuclease (SaCas9). The erythroid colonies differentiated from HSPCs with HPFH deletion showed significantly higher γ-globin gene expression compared with the colonies without deletion. By T7 endonuclease 1 assay, we did not detect any off-target effects in the colonies with deletion. We propose that this strategy of using nonhomologous end joining (NHEJ) to modify the genome may provide an efficient approach toward the development of a safe autologous transplantation for patients with homozygous β-thalassemia and SCD.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 6
    Publication Date: 2017-06-27
    Print ISSN: 2470-0010
    Electronic ISSN: 2470-0029
    Topics: Physics
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  • 7
  • 8
    Publication Date: 2018-01-31
    Description: Derivation of human hematopoietic stem cells (HSCs) from induced pluripotent stem cells (iPSCs) offers considerable promise for cell therapy, disease modeling, and drug screening. However, efficient derivation of functional iPSC-derived HSCs with in vivo engraftability and multilineage potential remains challenging. Here, we demonstrate a tractable approach for respecifying iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells (HSPCs) through transient expression of a single transcription factor, MLL-AF4. These induced HSPCs (iHSPCs) derived from iPSCs are able to fully reconstitute the human hematopoietic system in the recipient mice without myeloid bias. iHSPCs are long-term engraftable, but they are also prone to leukemic transformation during the long-term engraftment period. On the contrary, primary HSPCs with the same induction sustain the long-term engraftment without leukemic transformation. These findings demonstrate the feasibility of activating the HSC network in human iPSC-derived blood cells through expression of a single factor and suggest iHSPCs are more genomically instable than primary HSPCs, which merits further attention.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 9
    Publication Date: 2019-04-19
    Description: Thin polymer films on hydrophobic substrates are susceptible to rupture and hole formation. This, in turn, initiates a complex dewetting process, which ultimately leads to characteristic droplet patterns. Experimental and theoretical studies suggest that the type of droplet pattern depends on the specific interfacial condition between the polymer and the substrate. Predicting the morphological evolution over long timescales and on the different length scales involved is a major computational challenge. In this study, a highly adaptive numerical scheme is presented, which allows for following the dewetting process deep into the nonlinear regime of the model equations and captures the complex dynamics, including the shedding of droplets. In addition, our numerical results predict the previously unknown shedding of satellite droplets during the destabilization of liquid ridges that form during the late stages of the dewetting process. While the formation of satellite droplets is well known in the context of elongating fluid filaments and jets, we show here that, for dewetting liquid ridges, this property can be dramatically altered by the interfacial condition between polymer and substrate, namely slip. This work shows how dissipative processes can be used to systematically tune the formation of patterns.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 10
    Publication Date: 2018-02-07
    Description: The decline of cognitive function occurs with aging, but the mechanisms responsible are unknown. Astrocytes instruct the formation, maturation, and elimination of synapses, and impairment of these functions has been implicated in many diseases. These findings raise the question of whether astrocyte dysfunction could contribute to cognitive decline in aging. We used the Bac-Trap method to perform RNA sequencing of astrocytes from different brain regions across the lifespan of the mouse. We found that astrocytes have region-specific transcriptional identities that change with age in a region-dependent manner. We validated our findings using fluorescence in situ hybridization and quantitative PCR. Detailed analysis of the differentially expressed genes in aging revealed that aged astrocytes take on a reactive phenotype of neuroinflammatory A1-like reactive astrocytes. Hippocampal and striatal astrocytes up-regulated a greater number of reactive astrocyte genes compared with cortical astrocytes. Moreover, aged brains formed many more A1 reactive astrocytes in response to the neuroinflammation inducer lipopolysaccharide. We found that the aging-induced up-regulation of reactive astrocyte genes was significantly reduced in mice lacking the microglial-secreted cytokines (IL-1α, TNF, and C1q) known to induce A1 reactive astrocyte formation, indicating that microglia promote astrocyte activation in aging. Since A1 reactive astrocytes lose the ability to carry out their normal functions, produce complement components, and release a toxic factor which kills neurons and oligodendrocytes, the aging-induced up-regulation of reactive genes by astrocytes could contribute to the cognitive decline in vulnerable brain regions in normal aging and contribute to the greater vulnerability of the aged brain to injury.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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