Publication Date:
2015-05-22
Description:
The major acid phosphatase fromLegionella pneumophila(LpMAP) belongs to the histidine acid phosphatase superfamily. It contains the characteristic histidine acid phosphatase (HAP) sequence motif RHGXRXP responsible for the hydrolysis of a phosphoryl group from phosphate monoesters under acidic conditions. Here, the crystallization and preliminary X-ray analysis of crystals ofLpMAP in the apo form and in complex with L-(+)-tartrate are described. By using the hanging-drop vapour-diffusion method, apoLpMAP andLpMAP–tartrate were crystallized in space groupP21, with unit-cell parametersa= 91.50,b= 56.48,c= 146.35 Å, β = 110.01°, and in space groupP1, with unit-cell parametersa= 55.51,b= 73.51 ,c= 98.78 Å, α = 78.82, β = 77.65, γ = 67.73°, respectively. Diffraction data were collected at 100 K and the phases were determined using the molecular-replacement method.
Electronic ISSN:
2053-230X
Topics:
Biology
,
Chemistry and Pharmacology
,
Geosciences
,
Physics
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