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  • enzyme activity  (3)
  • cytochrome c  (2)
  • frequency measurement  (2)
  • ASTRONOMY  (1)
  • 2015-2019
  • 1995-1999  (8)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Encapsulation of Proteins in Bulk and Thin Film Sol-Gel Matrices (1997)
    Springer
    Journal of sol gel science and technology 8 (1997), S. 629-634 
    Details
    ISSN: 1573-4846
    Keywords: protein encapsulation ; absorption spectroscopy ; thin films ; cytochrome c
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract This paper considers the nature of the interactions between the sol-gel derived inorganic matrix and a specific biomolecule, cytochrome c. Optical absorption and impedance spectroscopies are used to characterize the influence of synthesis conditions on the protein's stability and conformation within the silica matrix. In some instances, encapsulation within the sol-gel matrix provides stabilization. For example, protein denaturation is reversible and aggregation is prevented. Moreover, the drying process does not negatively affect the protein; it is possible to regenerate the aged gel state by rehydration. The flexibility of the sol-gel process enables high quality cytochrome c-doped SiO2 thin films to be prepared. These films possess the characteristic reactivity and chemical function of cytochrome c in solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018316108875
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Spectroscopic Determination of Cholinesterase Activity and Inhibition in Sol-Gel Media (1997)
    Springer
    Journal of sol gel science and technology 8 (1997), S. 1067-1070 
    Details
    ISSN: 1573-4846
    Keywords: cholinesterase ; sol-gel ; pesticide ; THA ; enzyme activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Biological activity of cholinesterases can be determined by optically monitoring the enzymatic reaction with indophenyl acetate, (N-4′-acetoxyphenyl)-4-quinone imine. At pH 8.0 cholinesterases hydrolyze this yellow dye to yield a blue reaction product. Cholinesterase inhibitors reduce the rate of this hydrolysis. Thus, by monitoring absorbance of the hydrolysis product at its maximum (630 nm) as a function of time, reaction rates of both cholinesterase activity and cholinesterase inhibition may be quantified spectroscopically. Using this technique, we measured the enzymatic activity of butyrylcholinesterase (BuChE) molecules encapsulated in tetramethyl orthosilicate (TMOS) silicate gel-glass prepared by hydrolysis and condensation. This activity is reduced, in a concentration-dependent manner, by the reversible cholinesterase inhibitors 1,5-bis(4-allyldimethyl-ammoniumphenyl) pentan- 3-one dibromide (BADAPP) and 9-amino-1,2,3,4-tetrahydroacridine (THA; tacrine, Cognex). The gel-glasses are rigid and compact, transparent, and porous enough to allow reagents to diffuse in and out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018350828807
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Encapsulation of proteins in bulk and thin film sol-gel matrices (1997)
    Springer
    Journal of sol gel science and technology 8 (1997), S. 629-634 
    Details
    ISSN: 1573-4846
    Keywords: protein encapsulation ; absorption spectroscopy ; thin films ; cytochrome c
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract This paper considers the nature of the interactions between the sol-gel derived inorganic matrix and a specific biomolecule, cytochrome c. Optical absorption and impedance spectroscopies are used to characterize the influence of synthesis conditions on the protein’s stability and conformation within the silica matrix. In some instances, encapsulation within the sol-gel matrix provides stabilization. For example, protein denaturation is reversible and aggregation is prevented. Moreover, the drying process does not negatively affect the protein; it is possible to regenerate the aged gel state by rehydration. The flexibility of the sol-gel process enables high quality cytochrome c-doped SiO2 thin films to be prepared. These films possess the characteristic reactivity and chemical function of cytochrome c in solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02436913
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Spectroscopic determination of cholinesterase activity and inhibition in sol-gel media (1997)
    Springer
    Journal of sol gel science and technology 8 (1997), S. 1067-1070 
    Details
    ISSN: 1573-4846
    Keywords: cholinesterase ; sol-gel ; pesticide ; THA ; enzyme activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Biological activity of cholinesterases can be determined by optically monitoring the enzymatic reaction with indophenyl acetate, (N-4′-acetoxyphenyl)-4-quinone imine. At pH 8.0 cholinesterases hydrolyze this yellow dye to yield a blue reaction product. Cholinesterase inhibitors reduce the rate of this hydrolysis. Thus, by monitoring absorbance of the hydrolysis product at its maximum (630 nm) as a function of time, reaction rates of both cholinesterase activity and cholinesterase inhibition may be quantified spectroscopically. Using this technique, we measured the enzymatic activity of butyrylcholinesterase (BuChE) molecules encapsulated in tetramethyl orthosilicate (TMOS) silicate gel-glass prepared by hydrolysis and condensation. This activity is reduced, in a concentration-dependent manner, by the reversible cholinesterase inhibitors 1,5-bis(4-allyldimethyl-ammoniumphenyl) pentan 3-one dibromide (BADAPP) and 9-amino-1,2,3,4-tetrahydroacridine (THA; tacrine, Cognex). The gel-glasses are rigid, and compact, transparent and porous enough to allow reagents to diffuse in and out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02436985
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Allosteric Regulation of Enzymatic Reactions in a Transparent Inorganic Sol-Gel Material (1999)
    Springer
    Journal of sol gel science and technology 15 (1999), S. 57-62 
    Details
    ISSN: 1573-4846
    Keywords: glutamate dehydrogenase ; allosteric regulators ; sol-gel ; enzyme activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Glutamate dehydrogenase is encapsulated in a transparent porous silicate matrix by using sol-gel techniques. The inorganic polymer is formed around the enzyme (MW 〉 300,000 D). The enzyme is active in the material, catalyzes the reaction of L-glutamate to 2-oxoglutarate and follows Michaelis-Menten kinetics. The allosteric regulators ADP and GTP inhibit or activate the reaction; at pH 6, GTP acts as a strong activator and ADP acts as an inhibitor. This system involves a complex series of interactions; the co-enzyme NAD+ is required for catalysis, large-scale conformational changes accompany the binding of the substrate and coenzyme to the enzyme, the activators/inhibitors must bind to the enzyme to regulate the reactions, and the substrates and products must diffuse through the matrix to and from the binding site. The influence of the unique matrix on the complex enzymatic system is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008713014152
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  • 6
    Electronic Resource
    Electronic Resource
    Laser Lines and Frequency Measurements of Fully Deuterated Isotopomers of Methanol (1998)
    Springer
    International journal of infrared and millimeter waves 19 (1998), S. 465-473 
    Details
    ISSN: 1572-9559
    Keywords: 12CD3OD ; 13CD3OD ; fully deuterated isotopomers ; new laser lines ; far-infrared laser ; frequency measurement ; CO2 laser pump
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Fully deuterated isotopomers of methanol (12CD3OD and 13CD3OD) were optically pumped with a CO2 laser. Five new far-infrared laser lines were discovered in 12CD3OD and 25 in 13CD3OD in the range 43.697 to 719.426 μm. The frequencies of these new and some previously reported laser lines, the pump offset, the relative polarization, the relative intensity, and the optimum pressure of operation were measured.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022511827416
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  • 7
    Electronic Resource
    Electronic Resource
    New Far-Infrared Laser Lines and Frequency Measurements of Acetaldehyde and Vinyl Fluoride (1998)
    Springer
    International journal of infrared and millimeter waves 19 (1998), S. 719-726 
    Details
    ISSN: 1572-9559
    Keywords: far-infrared laser ; frequency measurement ; acetaldehyde (CH3CHO) ; vinyl fluoride (CH2CHF) ; cw CO2 laser ; new lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Eight far-infrared laser lines have been obtained by optically pumping acetaldehyde (CH3CHO) and nine by pumping vinyl fluoride (CH2CHF) with a cw CO2 laser. The far-infrared laser structure used a metal-dielectric waveguide cavity. This is the first reported observation of four of the laser lines in acetaldehyde. In this work, we measure the frequency, optimum pressure of operation, relative intensity, relative polarization, and pump offset from CO2 laser-line center.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022646225018
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    Paper (German National Licenses)
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  • 8
    facet.materialart.
    Unknown
    Far-infrared thermal emission from the inner cooling flow region of NGC 1275 (1995)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Measurements of the spatial distribution of 100 micrometers emission are presented for NGC 1275, the central galaxy in the Perseus cluster. The emission is clearly resolved on a kiloparsec level, and has the same flux density as seen by IRAS at an epoch when the nonthermal emission was a factor of 10 higher. This emission which traces the greater part of the luminosity of this galaxy, is thus identified as thermal emission from dust. The emission appears to be distributed more nearly like the low-velocity H-alpha filaments in the core of this galaxy than the starlight, extended nonthermal radio, X-ray emission, or high velocity gas. While the dust might be heated by a star-formation region that is fed by the cooling flow in this cluster, the intracluster gas also appears to be energetically capable of this as well, in which case the dust would be, at least at the present time, the dominant cooling mechanism for the hot gas. The large quantity of dust in NGC 1275 is unlikely to have been created within the galaxy, or in the cooling flow, but was probably accreted from a recent galactic interloper.
    Keywords: ASTRONOMY
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 439; 1; p. 185-190
    Format: text
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