Publication Date:
2016-09-09
Description:
ABSTRACT In this study, we created porcineinduced pluripotent stem (iPS) cells with the expression of six reprogramming factors ( Oct3/4 , Klf4 , Sox2 , c-Myc , Lin28 , and Nanog ).The resulting cells showed growth dependenton LIF (leukemia inhibitory factor) and expression of multiple stem cell markers.Furthermore, the iPS cells caused teratoma formation with three layers of differentiation and had both activeX chromosomes (XaXa).Our iPS cells satisfied the both of important characteristics of stem cells:teratoma formation and activation of both X chromosomes.Injection of these iPS cells into morula stage embryos showed that these cells participate in the early stage of porcine embryogenesis.Furthermore, the RNA-Seq analysis detected that expression levels of endogenous pluripotent related genes, NANOG , SOX2 , ZFP42 , OCT3/4 , ESRRB , and ERAS were much higher in iPS with six factors than that with four reprogramming factors.We can conclude that the expression of six reprogramming factors enables thecreation of porcine iPS cells, which is partially close to naive iPS state. This article is protected by copyright. All rights reserved
Electronic ISSN:
0091-7419
Topics:
Biology
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Chemistry and Pharmacology
,
Medicine
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