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  • Articles  (71)
  • 2015-2019  (25)
  • 1995-1999  (24)
  • 1990-1994  (22)
  • Biology  (71)
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  • Articles  (71)
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  • 1
    Electronic Resource
    Electronic Resource
    Effect of onion (Allium cepa) root exudates on the hyphal growth of Gigaspora margarita (1995)
    Springer
    Mycorrhiza 6 (1995), S. 57-59 
    Details
    ISSN: 1432-1890
    Keywords: Key words Arbuscular mycorrhizae ; Gigaspora margarita ; Allium cepa ; Phosphorus ; Root exudate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The effect of root exudates from onions differing in P status on spore germination and hyphal growth of arbuscular mycorrhizal fungi was investigated. Onion (Allium cepa) was grown in solution culture at different phosphorus concentrations (0, 0.1, 1.0, 8.0 and 24.0 mg P l–1) and root exudates were collected. When spores of the arbuscular mycorrhizal fungus, Gigaspora margarita were incubated with these root exudates, spore germination was only slightly affected but hyphal growth was greatly affected, particularly with exudates from P-deficient plants. This suggests that the P nutrition of host plants influences the composition of root exudates and thereby the hyphal growth of arbuscular mycorrhizal fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720050106
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  • 2
    Electronic Resource
    Electronic Resource
    Glutamate induces Cl− and K+ currents in the olfactory interneurons of a terrestrial slug (1999)
    Springer
    Journal of comparative physiology 184 (1999), S. 553-562 
    Details
    ISSN: 1432-1351
    Keywords: Key words Terrestrial slug ; Procerebrum ; Glutamate receptors ; Quisqualate ; Ibotenate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Glutamate-induced responses in the procerebral neurons of the terrestrial slug Limax marginatus were examined using the nystatin-perforated patch recording technique applied in the voltage-clamp mode and local application of drugs. The procerebrum contains two types of interneurons with different spontaneous activities, bursting and nonbursting neurons. In the bursting neurons, a puff of glutamate evoked a rapidly desensitizing current followed by a smaller sustained current. The reversal potential of the early component showed that the current was mediated by Cl− ions, while the late component was presumed to be mediated by K+ ions. In the nonbursting neurons, glutamate evoked a sustained current with a strong outward rectification, and the current was mediated by K+ ions. Ibotenate selectively evoked the rapidly desensitizing response in the bursting neurons, whereas quisqualate evoked a non-desensitizing K+ current both in the bursting and nonbursting neurons. The glutamate-induced K+ current had similar characteristics with the spontaneous synaptic activities in the procerebrum neurons, suggesting the possibility that glutamate receptors are involved in the spontaneous oscillatory activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003590050355
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  • 3
    Electronic Resource
    Electronic Resource
    Phase advances of circadian rhythms in somatostatin depleted rats: effects of cysteamine on rhythms of locomotor activity and electrical discharge of the suprachiasmatic nucleus (1994)
    Springer
    Journal of comparative physiology 175 (1994), S. 677-685 
    Details
    ISSN: 1432-1351
    Keywords: Circadian rhythm ; Suprachiasmatic nucleus ; Cysteamine ; Somatostatin ; Brain slice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Somatostatin is synthesized in the suprachiasmatic nucleus (SCN), a circadian pacemaker in mammals. To explore the functional significance of somatostatin in the circadian system, we examined rhythms of rat locomotor activity and electrical firing rate of SCN neurons in the brain slice after temporal depletion of somatostatin levels in the SCN. Intraperitoneal administration of cysteamine (200 mg/kg), a somatostatin depletor, significantly reduced somatostatin level in the in vivo SCN 5 min after injection and kept low level as long as 3 to 4 days. This administration, on the other hand, induced significant phase advances of about 51 min in the subsequent free-running rhythm of locomotor activity of the rat. A marked phase advance in the circadian rhythm of firing rate in the SCN was also observed after administration of cysteamine in coronal hypothalamic slices. These persistent phase shifts after administration of a somatostatin depletor may suggest that the change of somatostatin level in the SCN have a feedback influence on the circadian pacemaker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191840
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  • 4
    Electronic Resource
    Electronic Resource
    The reeler gene encodes a protein with an EGF–like motif expressed by pioneer neurons (1995)
    [s.l.] : Nature Publishing Group
    Nature genetics 10 (1995), S. 77-83 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] We have identified a strong candidate cDNA for the mouse reeler gene. This 5 kb transcript encodes a 99.4 kD protein consisting of 881 amino acids and possessing two EGF–like motifs. We assayed two independent mutant alleles — ‘Jackson reeler’, which has a deletion of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng0595-77
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  • 5
    Electronic Resource
    Electronic Resource
    Enhancement of protein secretion by optimizing protein synthesis: isolation and characterization of Escherichia coli mutants with increased secretion ability of alkaline phosphatase (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 163-169 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Overexpression of the Escherichia coli phoA gene, coding for alkaline phosphatase, on multicopy plasmids caused the accumulation of the precursor form of alkaline phosphatase. The cells lost their viability by a half-life of 60 min and exhibited high sensitivity to 1% sodium dodecyl sulfate (SDS), suggesting that the assembly of the surface proteins were affected by overexpression of the phoA gene. From the cells exhibiting resistance to 1% SDS, we obtained 20 mutants that secrete more alkaline phosphatase into the periplasmic space. Three representatives of the mutants accumulated no precursor molecules and secreted alkaline phosphatase by five- to six-fold that of the wild-type cells carrying multicopy phoA. In all of the three mutants, the amount of phoA transcripts were two to four times less than those in the wild-type cells, indicating that the ability to secrete a large amount of alkaline phosphatase was conferred by decreasing the synthetic rates of the phoA gene product. When the promoter of phoA was replaced with the tacI promoter and the expression level of the phoA gene was regulated with isopropyl-1-thio-β-d-galactoside, the secretion of alkaline phosphatase into the periplasm decreased as the synthetic rate of the phoA gene product increased over a threshold. All these results indicate that overproduction of the phoA gene product causes defects in the secretion of alkaline phosphatase and that the regulation of the expression level is essential for efficient translocation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186954
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  • 6
    Electronic Resource
    Electronic Resource
    Enhancement of protein secretion by optimizing protein synthesis: isolation and characterization of Escherichia coli mutants with increased secretion ability of alkaline phosphatase (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 163-169 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Overexpression of the Escherichia coli phoA gene, coding for alkaline phosphatase, on multicopy plasmids caused the accumulation of the precursor form of alkaline phosphatase. The cells lost their viability by a half-life of 60 min and exhibited high sensitivity to 1% sodium dodecyl sulfate (SDS), suggesting that the assembly of the surface proteins were affected by overexpression of the phoA gene. From the cells exhibiting resistance to 1% SDS, we obtained 20 mutants that secrete more alkaline phosphatase into the periplasmic space. Three representatives of the mutants accumulated no precursor molecules and secreted alkaline phosphatase by five- to six-fold that of the wild-type cells carrying multicopy phoA. In all of the three mutants, the amount of phoA transcripts were two to four times less than those in the wild-type cells, indicating that the ability to secrete a large amount of alkaline phosphatase was conferred by decreasing the synthetic rates of the phoA gene product. When the promoter of phoA was replaced with the tacI promoter and the expression level of the phoA gene was regulated with isopropyl-1-thio-β-D-galactoside, the secretion of alkaline phosphatase into the periplasm decreased as the synthetic rate of the phoA gene product increased over a threshold. All these results indicate that overproduction of the phoA gene product causes defects in the secretion of alkaline phosphatase and that the regulation of the expression level is essential for efficient translocation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050125
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  • 7
    Electronic Resource
    Electronic Resource
    Detection of manganese superoxide dismutase mRNA in the theca interna cells of rat ovary during the ovulatory process by in situ hybridization (1994)
    Springer
    Histochemistry and cell biology 102 (1994), S. 173-176 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To determine the possible involvement of reactive oxygen species in ovulation, dynamic aspects of superoxide dismutase (SOD) isozyme were studied in the ovaries of rats by in situ hybridization histochemistry. Previously, mRNA levels of ovarian manganese superoxide dismutase (Mn-SOD) were reported markedly to increase whilst enzymic activity of Mn-SOD decreased during the ovulatory process after treating immature rats with 10 and 5 Units, respectively, of pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG). Levels of Cu/Zn-SOD activity and Cu/Zn-SOD mRNA were reported to remain unchanged throughout ovulation. This increase in the Mn-SOD mRNA level was shown in the present study by in situ hybridization to be localized to the theca interna cells throughout the PMSG/HCG-induced ovulatory process. The observations suggest that the turnover rate of Mn-SOD but not Cu/Zn-SOD increases specifically in the mitochondria of these cells. SOD has been postulated to play important roles in steroidogenesis. The relationship is discussed between mitochondrial functions in steroid-secreting cells and superoxide radicals and related metabolite(s).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268893
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  • 8
    Electronic Resource
    Electronic Resource
    Discrimination of video images of conspecific individuals in bengalese finches (1993)
    Springer
    Journal of ethology 11 (1993), S. 67-72 
    Details
    ISSN: 1439-5444
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02350008
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  • 9
    Electronic Resource
    Electronic Resource
    Enzymatic synthesis of terpenyl esters by transesterification with fatty acid vinyl esters as acyl donors by Trichosporon fermentans lipase (1997)
    Springer
    World journal of microbiology and biotechnology 14 (1997), S. 219-222 
    Details
    ISSN: 1573-0972
    Keywords: Citronellyl ester ; fatty acid vinyl ester ; geranyl ester ; lipase ; transesterification ; Trichosporon fermentans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Enzymatic synthesis of terpenyl esters by esterification or transesterification with fatty acid vinyl esters as acyl donors by celite-adsorbed lipase of Trichosporon fermentans was investigated. In direct esterification of geraniol, the lipase showed high reactivity toward fatty acids with carbon chains longer than C-8, but little reactivity toward fatty acids with shorter chains. With fatty acid vinyl esters as acyl donors, the lipase catalysed the synthesis of geranyl and citronellyl esters with carbon chains shorter than C-6 in with yields of 〉90% molar conversion. Time course, effects of added water, temperature and substrate concentration were studied for the synthesis of geranyl acetate. Molar conversion yield reached 97.5% after 5 h incubation at 30–40°C with the addition of 3% water. In this reaction, no inhibition by substrates such as geraniol and vinyl acetate was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008882113333
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  • 10
    Electronic Resource
    Electronic Resource
    Phenylalanine ammonia lyase fromSporidiobolus pararoseus andRhodosporidium toruloides: Application for phenylalanine and tyrosine deamination (1992)
    Springer
    World journal of microbiology and biotechnology 8 (1992), S. 406-410 
    Details
    ISSN: 1573-0972
    Keywords: Deamination ; phenylalanine ammonia lyase ; phenylketonuria ; Rhodosporidium toruloides ; Sporidiobolus pararoseus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Simultaneous depletion of phenylalanine and tyrosine by phenylalanine ammonia lyase is described in a mutual competitive inhibition model. The enzymes obtained fromSporidiobolus pararoseus andRhodosporidium toruloides were charaterized in terms of stability, optimal reaction parameters and kinetic behaviour. Both enzymes followed Michaelis-Menten kinetics with respect to the two amino acids. However, the enzyme fromRhodosporidium toruloides was inhibited by high tyrosine concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01198755
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