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  • Solid-State Physics  (6)
  • Kuroshio  (2)
  • 162-980A; 162-980B; 162-980C; AGE; Boron/Calcium ratio; Carbonate ion; Date/Time of event; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Event label; Half; Isotope ratio mass spectrometry; Joides Resolution; Latitude of event; Leg162; Longitude of event; Ocean Drilling Program; ODP; pH; Sample code/label; South Atlantic Ocean; Standard deviation; δ11B  (1)
  • 2015-2019  (3)
  • 2000-2004  (6)
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  • 1
    Publication Date: 2024-01-09
    Keywords: 162-980A; 162-980B; 162-980C; AGE; Boron/Calcium ratio; Carbonate ion; Date/Time of event; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Event label; Half; Isotope ratio mass spectrometry; Joides Resolution; Latitude of event; Leg162; Longitude of event; Ocean Drilling Program; ODP; pH; Sample code/label; South Atlantic Ocean; Standard deviation; δ11B
    Type: Dataset
    Format: text/tab-separated-values, 383 data points
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  • 2
    Publication Date: 2019-07-18
    Description: Canavalin crystals grown from material purified and not purified by High Performance Liquid Chromatography were studied by atomic force microscopy and x-ray diffraction. After purification, resolution was improved from 2.55Angstroms to 2.22Angstroms and jagged isotropic spiral steps transformed into regular, well polygonized steps.
    Keywords: Solid-State Physics
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  • 3
    Publication Date: 2019-07-18
    Description: High angular-resolution x-ray diffraction and phase contrast x-ray imaging were combined to study defects and perfection of protein crystals. Imperfections including line defects, inclusions and other microdefects were observed in the diffraction images of a uniformly grown lysozyme crystal. The observed line defects carry distinct dislocation features running approximately along the 〈110〉 growth front and have been found to originate mostly in a central growth area and occasionally in outer growth regions. Slow dehydration led to the broadening of a fairly symmetric 4 4 0 rocking curve by a factor of approximately 2.6, which was primarily attributed to the dehydration-induced microscopic effects that are clearly shown in diffraction images. X-ray imaging and diffraction characterization of the quality of apoferritin crystals will also be discussed in the presentation.
    Keywords: Solid-State Physics
    Type: American Crystallographic Association Annual Meeting; Jul 17, 2004 - Jul 22, 2004; Chicago, IL; United States
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  • 4
    Publication Date: 2019-07-18
    Description: Ferritin is a well-known iron-storage protein, and is a spherical shell that consists of 24 identical subunits packed in a 432 symmetry. The typically large protein size and its distinction from lysozyme as to chemical and physical characteristics make ferritin an attractive model protein for crystal growth and perfection investigation-as an alternative to the most widely studied lysozyme. In this contribution, the latest results obtained from coherence-based x-ray diffraction imaging and diffraction experiments will be presented on octahedral apoferritin (a demetalized form of ferritin) crystals grown from various growth conditions. Crystal specimens, which have the measured rocking-curve widths varying from a few arcseconds to several tens arcseconds (or more), are comparatively examined by intrinsically highly sensitive mapping of lattice perfection and defects. The richness of the observed defects and growth features offers insight into perfection and growth of protein crystals. Beautiful interference fringe patterns formed in diffraction images and fine oscillation structure of rocking curves observed will be discussed for understanding of physical origins and the underlying impact.
    Keywords: Solid-State Physics
    Type: American Crystallographic Association Conference; Jul 26, 2003 - Jul 31, 2003; Covington, KY; United States
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  • 5
    Publication Date: 2019-07-18
    Description: Characterization of defects and/or disorder in biological macromolecular crystals presents much greater challenges than in conventional small-molecule crystals. The lack of sufficient contrast of defects is often a limiting factor in x-ray diffraction topography of protein crystals. This has seriously hampered efforts to understand mechanisms and origins of formation of imperfections, and the role of defects as essential entities in the bulk of macromolecular crystals. In this report, we employ a phase sensitive x-ray diffraction imaging approach for augmenting the contrast of defects in protein crystals.
    Keywords: Solid-State Physics
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  • 6
    Publication Date: 2019-07-17
    Description: Colorless transparent apoferritin (Mr = 450KDa) crystals have been grown from gel with Cd(2+) as precipitant in the presence of reddish brown-colored ferritin dimers (Mr = 900KDa). In agreement with our previous measurements, showing preferential trapping of dimers (distribution coefficient K = 4), the apoferritin crystals become strongly colored while the gel solution around them became nearly colorless. The depth of the depletion with respect to the colored dimer impurity allowed us to visualize the impurity depletion zone. Depletion with respect to impurity as compared to the crystallizing protein is discussed.
    Keywords: Solid-State Physics
    Type: ICCBM8 Conference; May 15, 2000; SanDestin, FL; United States
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  • 7
    Publication Date: 2019-07-17
    Description: Hen egg white lysozyme (HEWL) was acetylated to modify molecular charge keeping the molecular size and weight nearly constant. Two derivatives, A and B, more and less acetylated, respectively, were obtained, separated, purified and added to the solution from which crystals of tetragonal HEWL crystals were grown. Amounts of the A or B impurities added were 0.76, 0.38 and 0.1 milligram per millimeter while HEWL concentration were 20, 30 and 40 milligram per milliliter. The crystals grown in 18 experiments for each impurity were dissolved and quantities of A or B additives in these crystals were analyzed by cation exchange high performance liquid chromatography. All the data for each set of 18 samples with the different impurity and regular HEWL concentrations is well described by one distribution coefficient K = 2.15 plus or minus 0.13 for A and K = 3.42 plus or minus 0.25 for B. The observed independence of the distribution coefficient on both the impurity concentration and supersaturation is explained by the dilution model described in this paper. It shows that impurity adsorption and incorporation rate is proportional to the impurity concentration and that the growth rate is proportional to the crystallizing protein in solution. With the kinetic coefficient for crystallization, beta = 5.10(exp -7) centimeters per second, the frequency at which an impurity molecule near the growing interface irreversibly joins a molecular site on the crystal was found to be 3 1 per second, much higher than the average frequency for crystal molecules. For best quality protein crystals it is better to have low microheterogeneous protein impurity concentration and high supers aturation.
    Keywords: Solid-State Physics
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  • 8
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 122 (2017): 3519–3542, doi:10.1002/2016JC012519.
    Description: Observations from two companion field programs—Origins of the Kuroshio and Mindanao Current (OKMC) and Observations of Kuroshio Transport Variability (OKTV)—are used here to examine the Kuroshio's temporal and spatial evolution. Kuroshio strength and velocity structure were measured between June 2012 and November 2014 with pressure-sensor equipped inverted echo sounders (PIESs) and upward-looking acoustic Doppler current profilers (ADCPs) deployed across the current northeast of Luzon, Philippines, and east of Taiwan with an 8 month overlap in the two arrays' deployment periods. The time-mean net (i.e., integrated from the surface to the bottom) absolute transport increases downstream from 7.3 Sv (±4.4 Sv standard error) northeast of Luzon to 13.7 Sv (±3.6 Sv) east of Taiwan. The observed downstream increase is consistent with the return flow predicted by the simple Sverdrup relation and the mean wind stress curl field over the North Pacific (despite the complicated bathymetry and gaps along the North Pacific western boundary). Northeast of Luzon, the Kuroshio—bounded by the 0 m s−1 isotach—is shallower than 750 dbar, while east of Taiwan areas of positive flow reach to the seafloor (3000 m). Both arrays indicate a deep counterflow beneath the poleward-flowing Kuroshio (–10.3 ± 2.3 Sv by Luzon and −12.5 ± 1.2 Sv east of Taiwan). Time-varying transports and velocities indicate the strong influence at both sections of westward propagating eddies from the ocean interior. Topography associated with the ridges east of Taiwan also influences the mean and time-varying velocity structure there.
    Description: Office of Naval Research (ONR) Grant Numbers: N00014-15-12593 , N00014-16-13069; Taiwan's Ministry of Science and Technology Grant Numbers: NSC 101-2611-M-002-018-MY3 , MOST 103-2611-M-002-011 , MOST 105-2119-M-002-042; ONR Grant Numbers: N00014-10-1-0308 , N00015-10-1-0469
    Description: 2017-11-02
    Keywords: Kuroshio ; PIES ; ADCP ; Eddies ; Western boundary current ; Altimetry
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 9
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2015. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 42 (2015): 8098–8105, doi:10.1002/2015GL065814.
    Description: The influence and fate of westward propagating eddies that impinge on the Kuroshio were observed with pressure sensor-equipped inverted echo sounders (PIESs) deployed east of Taiwan and northeast of Luzon. Zero lag correlations between PIES-measured acoustic travel times and satellite-measured sea surface height anomalies (SSHa), which are normally negative, have lower magnitude toward the west, suggesting the eddy-influence is weakened across the Kuroshio. The observational data reveal that impinging eddies lead to seesaw-like SSHa and pycnocline depth changes across the Kuroshio east of Taiwan, whereas analogous responses are not found in the Kuroshio northeast of Luzon. Anticyclones intensify sea surface and pycnocline slopes across the Kuroshio, while cyclones weaken these slopes, particularly east of Taiwan. During the 6 month period of overlap between the two PIES arrays, only one anticyclone affected the pycnocline depth first at the array northeast of Luzon and 21 days later in the downstream Kuroshio east of Taiwan.
    Description: Ministry of Science and Technology (MOST) of Taiwan Grant Number: NSC-101-2611-M-002-018-MY3; US Office of Naval Research (ONR) Grant Number: N00014-12-1-0445; MA Grant Number: N00014-15-1-2593; ONR Grant Numbers: N00014-10-1-0397, N00014-10-1-0308, N00014-10-1-0468
    Description: 2016-03-08
    Keywords: Kuroshio ; Mesoscale eddy ; Eddy-Kuroshio interaction ; Pressure sensor-equipped inverted echo sounder
    Repository Name: Woods Hole Open Access Server
    Type: Article
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