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  • 42.60.Cz  (1)
  • 87  (1)
  • Springer  (2)
  • American Institute of Physics (AIP)
  • 2015-2019
  • 2000-2004
  • 1990-1994
  • 1980-1984  (2)
Collection
Keywords
Publisher
  • Springer  (2)
  • American Institute of Physics (AIP)
Years
  • 2015-2019
  • 2000-2004
  • 1990-1994
  • 1980-1984  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    A novel method of DNA transfection by laser microbeam cell surgery (1984)
    Springer
    Applied physics 35 (1984), S. 135-140 
    Details
    ISSN: 1432-0649
    Keywords: 07.60 ; 42.80 ; 87
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697702
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Stark modulation spectrometer using a wideband Zeeman-tuned He-Xe laser (1981)
    Springer
    Applied physics 24 (1981), S. 187-195 
    Details
    ISSN: 1432-0630
    Keywords: 32.20.Dr ; 42.60.Cz ; 42.60.Lh
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A 200 GHz-tunable 3.51 μm He−Xe laser is employed in Stark-modulation spectroscopy, and good performance of the spectrometer is demonstrated by an exemplary measurement on the HDO spectrum. Various spectral features and the anomalous line shapes due to the Stark effect are interpreted and used as an important clue to identification of the absorption lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00899754
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    Paper (German National Licenses)
    Fulltext
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