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  • Articles  (142)
  • 2015-2019  (35)
  • 2010-2014  (59)
  • 1990-1994  (16)
  • 1985-1989  (11)
  • 1980-1984  (16)
  • 1965-1969  (5)
  • Biology  (142)
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  • Articles  (142)
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  • 1
    Electronic Resource
    Electronic Resource
    Structure of the Escherichia coli K2 capsular antigen. Stereochemical configuration of the glycerophosphate and distribution of galactopyranosyl and galactofuranosyl residues (1982)
    s.l. : American Chemical Society
    Biochemistry 21 (1982), S. 1279-1284 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00535a027
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  • 2
    Electronic Resource
    Electronic Resource
    AIDA-I, the adhesin involved in diffuse adherence of the diarrhoeagenic Escherichia coli strain 2787 (O126:H27), is synthesized via a precursor molecule (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The adherence mechanisms of enteropathogenic Escherichia coli (EPEC) to epithelial cells are still not understood. To study the molecular basis of the diffuse adherence (DA) phenotype exhibited by diarrhoeagenic E. coli expressing classical EPEC serotypes we investigated strain 2787 (O126:H27) isolated from a case of infantile diarrhoea. A 6.0 kb plasmid-derrved DNA fragment mediates the DA phenotype and encodes the 100 kDa adhesin protein AIDA-I (adhesin involved in diffuse adherence). Sequencing of the entire fragment revealed two open reading frames which encoded proteins of 45 kDa and 132 kDa, respectively. The 132 kDa protein has been identified as an AIDA-I precursor protein. After cleavage of the signal sequence further processing at the C-terminus of the 132 kDa precursor leads to the mature ∼100 kDa AIDA-I. While the exact function of the cytopiasmic 45 kDa protein is not known, preliminary evidence indicates that it is necessary for the correct maturation of AIDA-I. The AIDA-l precursor exhibits significant homology with the virG(icsA) protein of Shigella flexneri which seems to be involved in the intercellular spread of invasive Shigella organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb00875.x
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of monoclonal antibodies directed against domains of pertussis toxin involved in receptor recognition (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 76 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The exotoxin pertussis toxin (PT) produced by virulent Bordetella pertussis bacteria is regarded as the main virulence factor of the organism and held responsible for most of its pathological effects. Identification of functional sites on PT would greatly facilitate site-specific detoxification and thus also the development of a new vaccine. For the investigation of structure-function aspects of PT we have prepared and characterized eleven monoclonal antibodies (mAbs) (UB-A1, UB-A2, UB-A10, UB-B7, UB-B12, UB-D4, UB-D7, UB-D10, UB-F7, UB-G1, and UB-G12) directed at the native toxin. Only UB-B12 and UB-D10 recognized PT in Western blotting indicating that most of the mAbs were directed against conformational epitopes. The mAbs were assayed for their ability to interfere with the binding of PT in model receptor systems like a solid phase binding assay using fetuin as receptor moiety, hemagglutination of chymotrypsin-sensitized goose erythrocytes, and the PT-mediated induction of the clustered growth pattern (CGP) of Chinese hamster ovary (CHO) cells. Five of the eleven mAbs (UB-A1, UB-A2, UB-B7, UB-B12, and UB-D7) interfered with the binding of PT to fetuin on solid phase and with PT-mediated hemagglutination. UB-A2, UB-B7, and UB-B12 also inhibited the induction of the clustered growth pattern of CHO-cells. This indicates that the determinants recognized by these mAbs are associated with the formation of the carbohydrate recognition sites of PT. Thus, the monoclonal antibodies described in this study will be valuable tools in the further analysis of the structure-function relationship of pertussis toxin with respect to receptor recognition and binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04223.x
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  • 4
    Electronic Resource
    Electronic Resource
    COMPARATIVE CHEMICAL ANALYSIS OF TWO VARIANTS OF THE ESCHERICHIA COLI K2 ANTIGEN (1980)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 7 (1980), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1980.tb01580.x
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  • 5
    Electronic Resource
    Electronic Resource
    Phospholipid substitution of capsular (K) polysaccharide antigens from Escherichia coli causing extraintestinal infections (1982)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 14 (1982), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1982.tb08637.x
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  • 6
    Electronic Resource
    Electronic Resource
    Are the funnel-canal organs the “campaniform sensilla” of the shore crab Carcinus maenas (Crustacea, Decapoda)? (1984)
    Springer
    Zoomorphology 104 (1984), S. 11-20 
    Details
    ISSN: 1432-234X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The topography of the funnel-canal organs of Carcinus maenas (Decapoda, Crustacea) and their stimulus-receiving cuticular and sensory apparatus were studied in the light and electron microscopes. About 4000 funnel-canal organs are situated within the exoskeleton of Carcinus. Almost all of them are on the distal segments of the walking legs, in particular on the epicuticular cap at the tip of the dactyl. They were not found to be arranged in groups or sensilla fields, and no sex-specific differences were observed. Characteristic features of the funnel-canal organs are as follows: (a) There is a terminal pore (0.5×0.8 μm diameter) in the cuticle, at the tip of a small projection. It is closed by a plug of electron-dense material. (b) The terminal sections of the dendrites are enclosed in a dendritic sheath up to ca. 10 μm below the pore. (c) The dendrites, 3–24 in number, end below the plug; none of the dendrites exhibits a tubular body; two of the dendrites are distinguished from the others by the greater number of microtubules in their outer segments. The structural characteristics, in particular the “gustatory pore” and the number of dendrites, are typical of bimodal receptors in arthropods. In such receptors, as in the contact chemoreceptors of insects and arachnids, mechano-and chemosensitive sensory cells are combined. This interpretation of the function of the funnel-canal organs is supported by electrophysiological data of other authors. The morphological parameters we find for the funnelcanal organs, in comparison with those of insect campaniform sensilla, provide clear evidence against the reclassification of the funnel-canal organs as “crustacean campaniform organs” proposed by Shelton and Laverack (1968).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312166
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  • 7
    Electronic Resource
    Electronic Resource
    Automatisches Herstellen von englumigen Glaskapillaren, Glasfäden und Mikropipetten (1966)
    Springer
    Naturwissenschaften 53 (1966), S. 582-582 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00600532
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  • 8
    Electronic Resource
    Electronic Resource
    Prevention of Cholestanol-induced Cholelithiasis by Neomycin (1965)
    [s.l.] : Nature Publishing Group
    Nature 208 (1965), S. 1226-1227 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Since a major step in the formation of gall-stones appears to be the bacterial 7-dehydroxylation of allocholic acid, it is reasonable to assume that the reduction or elimination of the bacteria responsible for the conversion might interfere with gall-stone formation. Because of its well-known ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2081226a0
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  • 9
    Electronic Resource
    Electronic Resource
    The hydration shell of myoglobin (1992)
    Springer
    European biophysics journal 21 (1992), S. 313-320 
    Details
    ISSN: 1432-1017
    Keywords: Monte Carlo ; Crystal water ; Structure refinement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The space in the unit cell of a metmyoglobin crystal not occupied by myoglobin atoms was filled with water using Monte Carlo calculations. Independent calculations with different amounts of water have been performed. Structure factors were calculated using the water coordinates thus obtained and the known coordinates of the myoglobin atoms. A comparison with experimental structure factors showed that both the low and the high resolution regime could be well reproduced with 814 Monte Carlo water molecules per unit cell with a B-value of 50 Å2. The Monte Carlo water molecules yield a smaller standard R-value (0.166) than using a homogeneous electron density for the simulation of the crystal water (R = 0.212). A reciprocal space refinement of the water and the protein coordinates has been performed. Monte Carlo calculations can be used to obtain information for crystallographically invisible parts of the unit cell and yield better coordinates for the visible part in the refinement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188343
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  • 10
    Electronic Resource
    Electronic Resource
    Über Versuche zur Fütterung der Kahlrückigen Waldameise (Formica polyctena F.; Hym., Formicidae) mit den behaarten Raupen vonOrgyia antiqua L. (Lep., Lymantriidae) (1985)
    Springer
    Journal of pest science 58 (1985), S. 70-72 
    Details
    ISSN: 1612-4766
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Zwei Laborpopulationen vonFormica polyctena (mit je 2000–3000 Arbeiterinnen und 2 Königinnen) wurden Raupen vonOrgyia antiqua als Nahrung vorgesetzt. Die L1 bis L3 wurden von den Ameisen nicht als Beute erkannt. Von den L4 bis L6 wurden von Population A=49% und von Population B =77% durch Verzehr oder Verletzung vernichtet. Um die lang behaarten Raupen töten zu können, „rupften” ihnen die Ameisen zuvor die Haare.
    Notes: Abstract O. antiqua larvae were given as food to two laboratory populations ofF. polyctena (2000–3000 workers and 2 queens each). The ants did'nt perceive L1–L3 as a prey. But they killed 47% (popul. A) and 77% (popul. B) of the L4–L6 after pulling up the long hairs of larvae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01903227
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