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  • Elsevier  (206)
  • Blackwell Science Ltd  (16)
  • 2020-2023  (2)
  • 2000-2004  (212)
  • 1955-1959  (6)
  • 1930-1934  (1)
  • 1910-1914  (1)
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  • 1
  • 2
    Publication Date: 2001-05-01
    Print ISSN: 0014-4827
    Electronic ISSN: 1090-2422
    Topics: Biology , Medicine
    Published by Elsevier
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  • 3
    Publication Date: 2001-08-01
    Print ISSN: 0167-4889
    Electronic ISSN: 1879-2596
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Published by Elsevier
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  • 4
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wehmiller, J. F., Brothers, L. L., Ramsey, K. W., Foster, D. S., Mattheus, C. R., Hein, C. J., & Shawler, J. L. Molluscan aminostratigraphy of the US Mid-Atlantic Quaternary coastal system: implications for onshore-offshore correlation, paleochannel and barrier island evolution, and local late Quaternary sea-level history. Quaternary Geochronology, 66, (2021): 101177, https://doi.org/10.1016/j.quageo.2021.101177.
    Description: The Quaternary record of the US Mid-Atlantic coastal system includes onshore emergent late Pleistocene shoreline deposits, offshore inner shelf and barrier island units, and paleovalleys formed during multiple glacial stage sea-level lowstands. The geochronology of this coastal system is based on uranium series, radiocarbon, amino acid racemization (AAR), and optically stimulated luminescence (OSL) methods. We report over 600 mollusk AAR results from 93 sites between northeastern North Carolina and the central New Jersey shelf, representing samples from both onshore cores or outcrops, sub-barrier and offshore cores, and transported shells from barrier island beaches. AAR age estimates are constrained by paired 14C analyses on specific shells and associated U-series coral ages from onshore sites. AAR data from offshore cores are interpreted in the context of detailed seismic stratigraphy. The distribution of Pleistocene-age shells on the island beaches is linked to the distribution of inner shelf or sub-barrier source units. Age mixing over a range of time-scales (~1 ka to ~100 ka) is identified by AAR results from onshore, beach, and shelf collections, often contributing insights into the processes forming individual barrier islands. The regional aminostratigraphic framework identifies a widespread late Pleistocene (Marine Isotope Stage 5) aminozone, with isolated records of middle and early Pleistocene deposition. AAR results provide age estimates for the timing of formation of the three major paleochannels that underlie the Delmarva Peninsula: Persimmon Point paleochannel ≥800 ka; Exmore paleochannel ~400–500 ka (MIS 12); and Eastville paleochannel 〉 125 ka (MIS 6). The results demonstrate the value of synthesizing abundant AAR chronologic data across various coastal environments, integrating multiple distinct geologic studies. The ages and elevations of the Quaternary units are important for current hypotheses about relative sea-level history and crustal dynamics in the region, which was likely influenced by the Laurentide ice sheet, the margin just ~400 km to the north.
    Description: This project was funded through a cooperative agreement with the Bureau of Ocean Energy Management of the U.S. Department of the Interior, Offshore Sand Resources for Coastal Resilience and Restoration Planning: M14AC00003 and M16AC00001. We thank J. Waldner (BOEM) for support and encouragement during this project. We also thank S. Howard and K. Luciano, South Carolina Geological Survey, and numerous colleagues in both the Mid-Atlantic and Southeast Atlantic BOEM ASAP projects, active from 2015 through 2019. This paper is contribution #3999 of the Virginia Institute of Marine Science, William & Mary. Partial support was also provided to Hein by the Mid-Atlantic Sea Grant program (NOAA) award numbers R/71856G and R/71856H and a Virginia Sea Grant (NOAA) Fellowship award NA18OAR4170083 supported Shawler. JFW acknowledges support from the University of Delaware Retired Faculty Research Program.
    Keywords: Quaternary sea-level ; Delmarva peninsula ; US Mid-Atlantic shelf ; Paleovalley ; Amino acid racemization ; Geochronology ; Age-mixing ; Seismic stratigraphy ; Mollusks
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 5
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Brothers, L. L., Foster, D. S., Pendleton, E. A., & Baldwin, W. E. Seismic stratigraphic framework of the continental shelf offshore Delmarva, USA: implications for Mid-Atlantic Bight evolution since the Pliocene. Marine Geology, 428, : (2020)106287, doi:10.1016/j.margeo.2020.106287.
    Description: Understanding how past coastal systems have evolved is critical to predicting future coastal change. Using over 12,000 trackline kilometers of recently collected, co-located multi-channel boomer, sparker and chirp seismic reflection profile data integrated with previously collected borehole and vibracore data, we define the upper (〈 115 m below mean lower low water) seismic stratigraphic framework offshore of the Delmarva Peninsula, USA. Twelve seismic units and 11 regionally extensive unconformities (U1-U11) were mapped over 5900 km2 of North America's Mid-Atlantic continental shelf. We interpret U3, U7, U9, U11 as transgressive ravinement surfaces, while U1,2,4,5,6,8,10 are subaerial unconformities illustrating distinct periods of lower sea-level. Based on areal distribution, stratigraphic relationships and dating results (Carbon 14 and amino acid racemization estimates) from earlier vibracore and borehole studies, we interpret the infilled channels as late Neogene and Quaternary courses of the Susquehanna, Potomac, Rappahannock, York, James rivers and tributaries, and a broad flood plain. These findings indicate that the region's geologic framework is more complex than previously thought and that Pleistocene paleochannels are abundant in the Mid-Atlantic. This study synthesizes and correlates the findings of other Atlantic Margin studies and establishes a large-scale Quaternary framework that enables more detailed stratigraphic analysis in the future. Such work has implications for inner continental shelf systems tract evolution, the relationship between antecedent geology and modern coastal systems, assessments of eustacy, glacial isostatic adjustment, and other processes and forcings that play a role in passive margin evolution.
    Description: This work was supported by the U.S. Department of the Interior's Response to Hurricane Sandy.
    Keywords: N Atlantic ; Shelf (morphology and stratigraphy) ; Quaternary stratigraphy ; Paleochannels ; Geophysics (seismic)
    Repository Name: Woods Hole Open Access Server
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  • 6
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The ability of Staphylococcus aureus cells to induce platelet aggregation has long been recognized. However, despite several attempts to identify the mechanisms involved in this interaction, the nature of the bacterial receptors required remains poorly understood. Using genetic manipulation, this study for the first time provides clear evidence that several S. aureus surface proteins participate in the inter-action with platelets. Mutants of S. aureus strain Newman lacking one or more surface proteins were tested for their ability to stimulate platelet aggre-gation. This approach was complemented by the expression of a number of candidate proteins in the non-aggregating Gram-positive bacterium Lacto-coccus lactis. S. aureus-induced aggregation was monophasic and was dependent on the platelet receptor GPIIb/IIIa. The fibrinogen-binding proteins, clumping factors A and B and the serine-aspartate repeat protein SdrE could each induce aggregation when expressed in L. lactis. Although protein A expressed in L. lactis was not capable of inducing aggregation independently, it enhanced the aggregation response when expressed on the surface of S. aureus. Thus, S. aureus has multiple mechanisms for stimulating platelet aggregation. Such functional redundancy suggests that this phenomenon may be important in the pathogenesis of invasive diseases such as infective endocarditis.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Enteropathogenic Escherichia coli (EPEC) is a major cause of infantile diarrhoea in a number of developing countries and is the prototype of pathogenic bacteria that cause attaching and effacing (A/E) intestinal lesions. A chromosomal pathogenicity island, termed the locus of enterocyte effacement (LEE), contains all the genes necessary for the A/E phenotype as well as genes for a type III secretion system and intimate adhesion. Genes in the LEE and genes involved in the synthesis of bundle-forming pili (BFP) are positively regulated by the plasmid-encoded regulator (Per) and comprise the per regulon. In order to identify factors that control the per regulon, we screened an EPEC genomic library for clones that modulate the expression of per. A plasmid clone that decreased the expression of per was isolated using a lacZ reporter gene fused to the per promoter. Subcloning revealed that YhiX, a putative AraC/XylR family transcriptional regulator, was the effector of per repression. Through downregulation of per, a plasmid overproducing YhiX reduced the synthesis of intimin, BfpA, Tir, and CesT, factors important for EPEC virulence. yhiX is located downstream of gadA, which encodes glutamate decarboxylase, an enzyme involved in acid resistance of E. coli. YhiX was found to be an activator of gadA, and the cloned yhiX gene increased production of glutamate decarboxylases (GAD) and activated the transcription of the gadA and gadB promoters. Therefore, yhiX was renamed gadX. Analysis of a gadX mutant grown in the different culture media with acidic and alkaline pH showed that regulation of perA, gadA and gadB by GadX was altered by the external pH and the culture media condition. Under conditions in which EPEC infects cultured epithelial cells, GadX negatively regulated perA expression, and the derepression in the gadX mutant increased translocation of Tir into epithelial cells relative to wild-type EPEC. DNA mobility shift experiments showed that purified GadX protein bound to the perA, gadA and gadB promoter regions in vitro, indicating that GadX is a transcriptional regulator of these genes. On the basis of these results, we propose that GadX may be involved in the appropriate expression of genes required for acid resistance and virulence of EPEC. Our data are consistent with a model in which environmental changes resulting from passage from the stomach to the proximal small intestine induce the functional effect of GadX on per and GAD expression in order to prevent inappropriate expression of the products of these two systems.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 51 (2004), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: As an important facet of host–pathogen interaction, Staphylococcus aureus has the ability to adhere to human extracellular matrix (ECM) components via a range of surface proteins. Here we have shown that IsdA has broad-spectrum ligand-binding activity, including fibrinogen and fibronectin. Mapping studies revealed a distinct domain responsible for ligand binding. This domain is present in a number of iron-regulated proteins of S. aureus and in other Gram-positive organisms. The isdA gene is only expressed in iron-limited conditions under the control of Fur and not in standard laboratory media. Such conditions occur in serum in vitro and during infection. Whole cell binding and clumping assays revealed that when the bacteria are grown under iron-limited conditions, IsdA constitutes a physiologically relevant adhesin to both fibrinogen and fibronectin. Thus for S. aureus, iron is an important marker for the host environment, to which the bacterium responds by differential regulation of at least one element of its adhesive strategy.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We report the complete sequence of Staphylococcal pathogenicity island bovine 2 (SaPIbov2), encoding the biofilm-associated protein Bap. SaPIbov2 contains 24 open reading frames, including sip, which encodes a functional staphylococcal integrase protein. SaPIbov2 is bordered by 18 bp direct repeats. The integration site into the chromosome lies at the 3′ end of a gene encoding GMP synthase. SaPIbov2 has extensive similarity to previously described pathogenicity islands of Staphylococcus aureus. The principal difference is that toxin genes present in the other pathogenicity islands are exchanged for a transposon-like element that carries the bap gene and genes encoding an ABC transporter and a transposase. Also, SaPIbov2 can be excised to form a circular element and can integrate site-specifically and RecA-independently at a chromosomal att site in a Sip-dependent manner. This was demonstrated both in S. aureus and with plasmid substrates ectopically in Escherichia coli. Thus, SaPIbov2 encodes a functional recombinase of the integrase family that promotes element excision and insertion/integration. In addition, we demonstrated that the presence of SaPIbov2 facilitated the persistence of S. aureus in an intramammary gland infection model. Finally, different bovine isolates of S. aureus were found to carry islands related to SaPIbov2, suggesting the existence of a family of related pathogenicity islands.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Journal of fish biology 65 (2004), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We present the first synthesis of the life history and ecology of seahorses, compare relationships for seahorses with other marine teleosts and identify research needs. Seahorses occurred primarily amidst temperate seagrasses and tropical coral reefs. Population densities were generally low, ranging from 0 to 0·51 individuals m−2, but reached 10 m−2 in some patches. Inferred life spans ranged from 1 to 5 years. Seahorses consumed live prey and possibly changed diet as they grew. Growth rates are poorly investigated to date. Reproduction and mating systems are the best-studied aspects of seahorse ecology. The relationship between size at first maturity and maximum size in seahorses conformed to that for other marine teleosts. All seahorse species were monogamous within a cycle, but some were polygamous across cycles. Direct transfer of clutches to the brood pouch of the male fish made it difficult to measure clutch size in live seahorses. After brooding, males released from c. 5 to 2000 young, depending on species and adult size. Newborn young measured from 2 to 20 mm in length, which was a narrower size range than the 17-fold difference that occurred in adult size. Newborn body size had no relationship to adult size. Both eggs and young were larger than expected among marine teleosts, even when considering only those with parental care, but brood size at release was lower than expected, perhaps because the young were more developed. The size of adults, eggs and young increased with increasing latitude, although brood size did not. Considerable research is needed to advance seahorse conservation and management, including (a) fisheries-dependent and fisheries-independent abundance estimates, (b) age- or stage-based natural and fishing mortalities, (c) growth rates and age at first maturity, and (d) intrinsic rates of increase and age- or size-specific reproductive output. Current data confirm that seahorses are likely to be vulnerable to high levels of exploitation.
    Type of Medium: Electronic Resource
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