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  • Articles  (643)
  • Oxford University Press  (235)
  • American Geophysical Union  (206)
  • American Society of Hematology  (202)
  • 2020-2023  (4)
  • 2005-2009  (373)
  • 1990-1994  (166)
  • 1980-1984  (94)
  • 1950-1954  (6)
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  • Articles  (643)
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  • 1
    Publication Date: 2009-07-09
    Description: Here we show that endothelial cells (EC) require matrix type 1-metalloproteinase (MT1-MMP) for the formation of lumens and tube networks in 3-dimensional (3D) collagen matrices. A fundamental consequence of EC lumen formation is the generation of vascular guidance tunnels within collagen matrices through an MT1-MMP-dependent proteolytic process. Vascular guidance tunnels represent a conduit for EC motility within these spaces (a newly remodeled 2D matrix surface) to both assemble and remodel tube structures. Interestingly, it appears that twice as many tunnel spaces are created than are occupied by tube networks after several days of culture. After tunnel formation, these spaces represent a 2D migratory surface within 3D collagen matrices allowing for EC migration in an MMP-independent fashion. Blockade of EC lumenogenesis using inhibitors that interfere with the process (eg, integrin, MMP, PKC, Src) completely abrogates the formation of vascular guidance tunnels. Thus, the MT1-MMP-dependent proteolytic process that creates tunnel spaces is directly and functionally coupled to the signaling mechanisms required for EC lumen and tube network formation. In summary, a fundamental and previously unrecognized purpose of EC tube morphogenesis is to create networks of matrix conduits that are necessary for EC migration and tube remodeling events critical to blood vessel assembly.
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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  • 2
    Publication Date: 2009-12-03
    Description: We show that endothelial cell (EC)–generated vascular guidance tunnels (ie, matrix spaces created during tube formation) serve as conduits for the recruitment and motility of pericytes along EC ablumenal surfaces to facilitate vessel maturation events, including vascular basement membrane matrix assembly and restriction of EC tube diameter. During quail development, pericyte recruitment along microvascular tubes directly correlates with vascular basement membrane matrix deposition. Pericyte recruitment to EC tubes leads to specific induction of fibronectin and nidogen-1 (ie, matrix-bridging proteins that link together basement membrane components) as well as perlecan and laminin isoforms. Coincident with these events, up-regulation of integrins, α5β1, α3β1, α6β1, and α1β1, which bind fibronectin, nidogens, laminin isoforms, and collagen type IV, occurs in EC-pericyte cocultures, but not EC-only cultures. Integrin-blocking antibodies to these receptors, disruption of fibronectin matrix assembly, and small interfering RNA suppression of pericyte tissue inhibitor of metalloproteinase (TIMP)-3 (a known regulator of vascular tube stabilization) all lead to decreased EC basement membrane, resulting in increased vessel lumen diameter, a key indicator of dysfunctional EC-pericyte interactions. Thus, pericyte recruitment to EC-lined tubes during vasculogenesis is a stimulatory event controlling vascular basement membrane matrix assembly, a fundamental maturation step regulating the transition from vascular morphogenesis to stabilization.
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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  • 3
  • 4
  • 5
    Publication Date: 2022-10-26
    Description: Author Posting. © American Geophysical Union, 2019. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 46(16), (2019): 9851-9860, doi:10.1029/2019GL083726.
    Description: Coral reef calcification is expected to decline due to climate change stressors such as ocean acidification and warming. Projections of future coral reef health are based on our understanding of the environmental drivers that affect calcification and dissolution. One such driver that may impact coral reef health is heterotrophy of oceanic‐sourced particulate organic matter, but its link to calcification has not been directly investigated in the field. In this study, we estimated net ecosystem calcification and oceanic particulate organic carbon (POCoc) uptake across the Kāne'ohe Bay barrier reef in Hawai'i. We show that higher rates of POCoc uptake correspond to greater net ecosystem calcification rates, even under low aragonite saturation states (Ωar). Hence, reductions in offshore productivity may negatively impact coral reefs by decreasing the food supply required to sustain calcification. Alternatively, coral reefs that receive ample inputs of POCoc may maintain higher calcification rates, despite a global decline in Ωar.
    Description: Data needed for calculations are available in the supporting information. Additional data can be provided upon request directly from the corresponding author or accessed by links provided in the supporting information. The authors declare no competing financial interests. We thank Texas Sea Grant for providing partial funding for this project to A. Kealoha through the Grants‐In‐Aid of Graduate Research Program. We also thank the NOAA Nancy Foster Scholarship for PhD program funding to A. Kealoha and Texas A&M University for funds awarded to Shamberger that supported this work. This research was also supported by funding from National Science Foundation Grant OCE‐1538628 to Rappé. The Hawaii Institute of Marine Biology (particularly the Rappé Lab and Jason Jones), NOAA's Coral Reef Ecosystem Program, Connie Previti, Serena Smith, and Chris Maupin were instrumental in sample collection and data analysis.
    Description: 2020-02-22
    Keywords: Coral reefs ; Ocean acidification ; Climate change ; Heterotrophy
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
    Publication Date: 2022-10-26
    Description: Author Posting. © American Geophysical Union, 2020. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 125(5), (2020): e2019JC015377, doi:10.1029/2019JC015377.
    Description: Internal waves strongly influence the physical and chemical environment of coastal ecosystems worldwide. We report novel observations from a distributed temperature sensing (DTS) system that tracked the transformation of internal waves from the shelf break to the surf zone over a narrow shelf slope region in the South China Sea. The spatially continuous view of temperature fields provides a perspective of physical processes commonly available only in laboratory settings or numerical models, including internal wave reflection off a natural slope, shoreward transport of dense fluid within trapped cores, and observations of internal rundown (near‐bed, offshore‐directed jets of water preceding a breaking internal wave). Analysis shows that the fate of internal waves on this shelf—whether transmitted into shallow waters or reflected back offshore—is mediated by local water column density structure and background currents set by the previous shoaling internal waves, highlighting the importance of wave‐wave interactions in nearshore internal wave dynamics.
    Description: We are grateful for the support of the Dongsha Atoll Research Station (DARS) and the Dongsha Atoll Marine National Park, whose efforts made this research possible. The authors would also like to thank A. Hall, S. Tyler, and J. Selker from the Center for Transformative Environmental Monitoring Programs (CTEMPs) funded by the National Science Foundation (EAR awards 1440596 and 1440506), G. Lohmann from WHOI, A. Safaie from UC Irvine, G. Wong, L. Hou, F. Shiah, and K. Lee from Academia Sinica for providing logistical and field support, as well as E. Pawlak, S. Lentz, B. Sanders, and S. Grant for equipment, and B. Raubenheimer, S. Elgar, R. Walter and D. Lucas for informative discussions that improved this work. We acknowledge the US Army Research Laboratory DoD Supercomputing Resource Center for computer time on Excalibur, which was used for the numerical simulations in this work. Funding for this work supported by Academia Sinica and for K.D. and E.R. from NSF‐OCE 1753317 and for O.F., J.R., and R.A. from ONR Grant 1182789‐1‐TDZZM. A portion of this work (R.A.) was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE‐AC52‐07NA27344.
    Description: 2020-10-21
    Keywords: Internal waves ; Distributed temperature sensing ; Coral reef ; Internal wave reflection
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 7
    Publication Date: 2022-10-26
    Description: Author Posting. © American Geophysical Union, 2020. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 125(12), (2020): e2020JC016543, https://doi.org/10.1029/2020JC016543.
    Description: On coral reefs, flow determines residence time of water influencing physical and chemical environments and creating observable microclimates within the reef structure. Understanding the physical mechanisms driving environmental variability on shallow reefs, which distinguishes them from the open ocean, is important for understanding what contributes to thermal resilience of coral communities and predicting their response to future anomalies. In June 2014, a field experiment conducted at Dongsha Atoll in the northern South China Sea investigated the physical forces that drive flow over a broad shallow reef flat. Instrumentation included current and pressure sensors and a distributed temperature sensing system, which resolved spatially and temporally continuous temperature measurements over a 3‐km cross‐reef section from the lagoon to reef crest. Spectral analysis shows that while diurnal variability was significant across the reef flat—a result expected from daily solar heating—temperature also varied at higher frequencies near the reef crest. These spatially variable temperature regimes, or thermal microclimates, are influenced by circulation on the wide reef flat, with spatially and temporally variable contributions from tides, wind, and waves. Through particle tracking simulations, we find the residence time of water is shorter near the reef crest (3.6 h) than near the lagoon (8.6 h). Tidal variability in flow direction on the reef flat leads to patterns in residence time that are different than what would be predicted from unidirectional flow. Circulation on the reef also determines the source (originating from offshore vs. the lagoon) of the water present on the reef flat.
    Description: We thank S. Tyler, and J. Selker from the Center for Transformative Environmental Monitoring Programs (CTEMPs), funded by the National Science Foundation (EAR awards 1440596 and 1440506), for timely and effective provision of experimental design support, logistical support and equipment for the project. Support for S. Lentz is from NSF Grant No. OCE‐1558343. Support for A. Cohen from NSF Grant No. 1220529, by the Academia Sinica (Taiwan) through a thematic project grant to G. Wong and A. Cohen. Support for E. Reid and K. Davis is from National Science Foundation (NSF) Grant No. OCE‐1753317, and support to E. Reid from the Environmental Engineering Henry Samueli Endowed Fellowship and the UCI Oceans Graduate Fellowship.
    Description: 2021-05-23
    Keywords: Coral reef ; Distributed temperature sensing ; Temperature variability
    Repository Name: Woods Hole Open Access Server
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  • 8
    Publication Date: 2022-10-20
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Davis, S. R., Farrar, J. T., Weller, R. A., Jiang, H., & Pratt, L. J. The land-sea breeze of the Red Sea: observations, simulations, and relationships to regional moisture transport. Journal of Geophysical Research-Atmospheres, 124, (2019): 13803-13825, doi: 10.1029/2019JD031007.
    Description: Unique in situ observations of atmospheric conditions over the Red Sea and the coastal Arabian Peninsula are examined to study the dynamics and regional impacts of the local land‐sea breeze cycle (LSBC). During a 26‐month data record spanning 2008–2011, observed LSBC events occurred year‐round, frequently exhibiting cross‐shore wind velocities in excess of 8 m/s. Observed onshore and offshore features of both the land‐ and sea‐breeze phases of the cycle are presented, and their seasonal modulation is considered. Weather Research and Forecasting climate downscaling simulations and satellite measurements are used to extend the analysis. In the model, the amplitude of the LSBC is significantly larger in the vicinity of the steeper terrain elements encircling the basin, suggesting an enhancement by the associated slope winds. Observed and simulated conditions also reflected distinct gravity‐current characteristics of the intrinsic moist marine air mass during both phases of the LSBC. Specifically, the advance and retreat of marine air mass was directly tied to the development of internal boundary layers onshore and offshore throughout the period of study. Convergence in the lateral moisture flux resulting from this air mass ascending the coastal topography (sea‐breeze phase) as well as colliding with air masses from the opposing coastline (land‐breeze phase) further resulted in cumulous cloud formation and precipitation.
    Description: This study was supported by National Science Foundation (NSF) Grant OCE‐1435665 and National Aeronautics and Space Administration (NASA) Grants 80NSSC18K1494 and NNX14AM71G. Further support for Lawrence Pratt was provided by NSF Grant OCE‐1154641. The authors wish to thank Sarah Gille for insightful conversations related to this work. GLDAS data used in this study were acquired as part of the mission of NASA's Earth Science Division and archived and distributed by the Goddard Earth Sciences (GES) Data and Information Services Center (DISC). We further acknowledge the use of data and imagery from LANCE FIRMS operated by the NASA/GSFC/Earth Science Data and Information System (ESDIS) with funding provided by NASA/HQ. The in situ data from the WHOI/KAUST mooring is available at a WHOI repository (http://uop.whoi.edu/projects/kaust/form.php) for academic and research purposes. The mooring data collected during the WHOI‐KAUST collaboration was made possible by awards USA00001, USA00002, and KSA00011 to WHOI by the KAUST in the Kingdom of Saudi Arabia. The buoy and tower data collection was a result of the work of the WHOI Upper Ocean Processes Group and staff at KAUST; John Kemp, Jason Smith, Paul Bouchard, Sean Whelan, Yasser Abualnaja, Yasser Kattan, and Abdulaziz Al‐Suwailem all made major contributions.
    Keywords: Sea‐breeze ; Land‐breeze ; Red Sea ; African coast ; Air‐sea ; Observations and modelling
    Repository Name: Woods Hole Open Access Server
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  • 9
    Publication Date: 1990-01-01
    Print ISSN: 0956-540X
    Electronic ISSN: 1365-246X
    Topics: Geosciences
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  • 10
    Publication Date: 1984-03-01
    Description: Tumor burden in adult patients with acute leukemia is assessed using the percentage of blast cells in the bone marrow or blood. It is clear, however, that not all blast cells are leukemic cells, especially during rapid marrow regeneration. Similarly, some leukemia cell lines have been shown to differentiate in vitro, and the same process also occurs in vivo. Therefore, the leukemic burden may be due to more differentiated cells as well as to blast cells. The purpose of this study was to investigate whether the human malignancy-associated nucleolar antigen (HMNA) could be used as a marker for leukemic cells and to examine its potential as a diagnostic tool. The proportion of HMNA-positive cells in the bone marrow of patients with acute leukemia was determined by indirect immunofluorescence with antibodies to HMNA and was compared with the differential counts routinely made in the clinic laboratory. The percentages of HMNA-positive cells among the nucleated cells in the marrow of 72 patients with clinical evidence of leukemia were significantly higher (range 9%-98%, median 83%) than those observed for nonleukemic individuals (range less than 0.05%-2.5%, median 1%) or for fractions of marrow cells from normal volunteers enriched for normal early progenitor cells (less than or equal to 2%). Patients with leukemia in remission had a lower percentage of HMNA- positive cells (range 0%-83%, median 3%). The percentage of HMNA- positive cells increased as patients approached relapse. Although the percentage of HMNA-positive cells was related to the percentage of blast cells in the bone marrow of the patients with leukemia, some partially differentiated cells were also HMNA-positive in some specimens, and some blastic cells were HMNA-negative in other specimens. These studies indicate the potential usefulness of HMNA as a marker for leukemic cells.
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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