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  • 1
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    Spinach and Chive for Kidney Tubule Engineering: the Limitations of Decellularized Plant Scaffolds and Vasculature (2020)
    Springer
    Details
    Publication Date: 2020-12-28
    Description: Tissue decellularization yields complex scaffolds with retained composition and structure, and plants offer an inexhaustible natural source of numerous shapes. Plant tissue could be a solution for regenerative organ replacement strategies and advanced in vitro modeling, as biofunctionalization of decellularized tissue allows adhesion of various kinds of human cells that can grow into functional tissue. Here, we investigated the potential of spinach leaf vasculature and chive stems for kidney tubule engineering to apply in tubular transport studies. We successfully decellularized both plant tissues and confirmed general scaffold suitability for topical recellularization with renal cells. However, due to anatomical restrictions, we believe that spinach and chive vasculature themselves cannot be recellularized by current methods. Moreover, gradual tissue disintegration and deficient diffusion capacity make decellularized plant scaffolds unsuitable for kidney tubule engineering, which relies on transepithelial solute exchange between two compartments. We conclude that plant-derived structures and biomaterials need to be carefully considered and possibly integrated with other tissue engineering technologies for enhanced capabilities.
    Print ISSN: 1522-1059
    Electronic ISSN: 1550-7416
    Topics: Chemistry and Pharmacology
    Published by Springer
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Effect of red light on irreversible and reversible expansion of Avena coleoptile sections (1966)
    Springer
    Planta 71 (1966), S. 291-304 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The increase in the plasticity of Avena coleoptile sections by auxin which has been found in external solutions with an osmotic value, lower than a critical value (Cleland, 1959) is observed only in sections from red-irradiated coleoptiles. Irradiation with red light of sections, taken from dark-grown seedlings and cut in dim green light, has no effect on the plasticity of the sections. 2. Different zones of the coleoptile show different responses to small and large doses of red light. 3. Exposure of the coleoptiles to red light during two minutes (Intensity 40 μW.cm-2) induces a short-term increase of plasticity of the sections (90 minutes after irradiation) in solutions of low osmotic value. This short-term effect of red light interferes with a long-term (16 hours after irradiation) decrease of the suction force of the sections and a decrease of plasticity over the whole range of osmotic values, between pure buffer and the value causing incipient plasmolysis. 4. Only the long-term reduction of plasticity is reversed by far-red irradiation applied after the red irradiation. 5. In most cases, the elastic stretching was not influenced by red light when applied 90 minutes before sectioning, but in some experiments it was increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384890
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  • 3
    Electronic Resource
    Electronic Resource
    An irreversible red-light-induced growth response in Avena (1968)
    Springer
    Planta 82 (1968), S. 87-104 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inhibition of the growth rate of the first internode of Avena by red light occurs in three steps. The first step reduces elongation by ca. 15%. It is produced and saturated by 10-3 to 10-1 μW sec cm-2 at λ=660 nm and is irreversible by far-red irradiation. All wavelengths between 400 and 800 nm produce and saturate this step. The second step, produced by red light quantities between ca. 10 and 104 μW sec cm-2 reduces elongation to ca. 50% of the maximal; it is not produced by far-red light but far-red reverses completely this component of the effect of red light. The third step inhibits mesocotyl elongation to ca. 95% of the maximal. The effect of red light in this step depends on the duration of irradiation rather than on the total quantity of energy, and is not reversed by far-red irradiation. The three inhibition steps in the elongation of the mesocotyl are matched by three growth-promotion steps in the growth of the coleoptile, but the extent of the far-red-irreversible first step outweighs in this case by far the extent of the far-red-reversible second step.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384699
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  • 4
    Electronic Resource
    Electronic Resource
    Calcitonin, its effect as measured in Paget's disease (1968)
    Springer
    Calcified tissue international 2 (1968), S. 22-22 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Conclusions (1) The relation between the lowering effects of Ct on plasma-Ca and-PO4 and bone turnover rate was confirmed in humans. (2) The changes in plasma measured after CT must be due to a decrease of bone resorption rate. (3) The changes of Ca-, PO4- and Mg-excretion are secondary to changes of renal tubular transport. (4) The change of hydroxyproline-excretion is secondary to the decrease of plasma-hydroxyproline.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02065204
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  • 5
    Electronic Resource
    Electronic Resource
    A computer simulated model of a second order sensory neurone (1969)
    Springer
    Biological cybernetics 6 (1969), S. 130-141 
    Details
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Summary The behaviour of neurones during signal transmission is determined partly by the input to the nerve cell and partly by its inherent properties. A detailed knowledge of the spike trains, which are input to and output from a synapse, may yield information about these synaptic mechanisms. This problem has been treated in relation to data from the dorsal spino-cerebellar tract (DSCT) cells monosynaptically activated from primary endings of muscle spindles. After a description of the firing pattern of these cells the behaviour of various models are examined by computer simulation. A particular type of model approximates the behaviour of the DSCT neurones closely with a rather narrow set of parameters. The model predicts that about 15 primary afferent fibres from one muscle converge on one DSCT cell and that the average size of their EPSPs may be as large as 50% of the threshold of firing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274106
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  • 6
    Electronic Resource
    Electronic Resource
    A biological model of the excitation of a second order sensory neurone (1969)
    Springer
    Biological cybernetics 6 (1969), S. 141-145 
    Details
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Summary Computer simulation of a relatively simple model can reproduce the main characteristics of the firing patters of some nerve cells. The abdominal stretch receptor of the crayfish has provided an analogous biological model. Synaptic input impulses were simulated by randomly distributed, short lasting transmembrane current pulses. Under these experimental conditions the stretch receptor neurone largely behaved as predicted by the computer simulations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274107
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  • 7
    Electronic Resource
    Electronic Resource
    Die Wirkung von Gibberellinsäure und Indolylessigsäure auf die Wurzelbildung von Tomatenstecklingen (1967)
    Springer
    Planta 74 (1967), S. 371-378 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Root formation of tomato cuttings was always inhibited by GA, never promoted. The number of roots was reduced and the roots appeared later, so that at the end of the experiment (13 days later) a reduced dry weight resulted. The effect depended on the concentration of GA, the lowest effective concentration being 10-3 mg/l. Root formation decreased up to 1000 mg/l GA. An effect on the shoot was noted at concentrations above 10 mg/l. Counts of root primordias after 3–5 days showed the same diminution of root formation. The growth of existing primordias seemed not to be influenced by GA. Short-time treatments with GA had a smaller effect, but a 3-day absorption-period resulted in the same decrease as a treatment during the whole experiment. When GA was given after the experiment had started, the first 3 days of treatment were again the most effective. This result shows that the induction of root primordias is inhibited or at least retarded. CCC had no effect on root formation nor was it able to alter the inhibition by GA. IAA alone did not promote the rooting process. In combination with GA (concentration range for both 10-3–1 mg/l) IAA never reduced the inhibition by GA. On the contrary, 1 mg/l IAA+GA was somewhat more inhibitive than GA alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389095
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  • 8
    Electronic Resource
    Electronic Resource
    Kriterien für das Fehlen quadratisch integrierbarer Lösungen der Differentialgleichung −Δv=f(x, v) in Außengebieten (1969)
    Springer
    Mathematische Annalen 183 (1969), S. 185-200 
    Details
    ISSN: 1432-1807
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01351379
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  • 9
    Electronic Resource
    Electronic Resource
    Dreikörperkräfte und die Stabilität einfacher Kristallgitter (1966)
    Springer
    The European physical journal 190 (1966), S. 161-190 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Observed stability of crystal structures for rare-gas atoms, alkali halides and for those II–VI and III–V compounds whose ions are isoelectronic with rare-gas atoms is correlated with atomic and ionic interactions in dense media. On the basis of central forces essential discrepancies between theory and experiment occur. Further analysis indicates that strong, simultaneous interactions of exchange type between three atoms or three ions play an important role in determining the stable crystal structure. An evaluation of such three-body interactions is carried out in first and second orders of perturbation theory, using effective-electron wave functions of Gaussian form. It is shown that the theory accounts for all observed stability relations on a quantitative basis. The stability of the face-centered cubic configuration for solids of heavy rare gases is explained. Also, the occurrence of the cesium chloride structure for some of the heavy alkali halides and the magnitude of observed transition pressures are reproduced by the theory. In addition, it is shown that three-ion interactions are responsable for the occurrence of the sphalerite (zincblende) and wurtzite configurations with II–VI and III–V compounds, without covalent bonding between the ions being involved. General laws are given which govern the relative stability of the sodium chloride, cesium chloride, sphalerite and wurtzite configurations for these types of compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01327141
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  • 10
    Electronic Resource
    Electronic Resource
    Inosinate pyrophosphorylase activity in immature blood cells in X-linked congenital hyperuricosuria (1969)
    Springer
    Biochemical genetics 3 (1969), S. 311-316 
    Details
    ISSN: 1573-4927
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Radioautographic examination of the bone marrow of three patients with X-linked congenital hyperuricosuria (Lesch-Nyhan's disease), after incubation with radioactive hypoxanthine, revealed no labeling even in extremely immature blood cells. An adequacy of the cosubstrate, phosphoribosepyrophosphate, in these cells was demonstrated by the appearance of labeling following incubation with tritiated adenine. It is concluded that the enzyme IMP pyrophosphorylase (inosinate: pyrophosphate phosphoribosyltransferase E.C.2.4.2.8) is deficient in young blood cells as well as in peripheral blood elements. The possibility that the enzyme deficiency in the peripheral blood results from the rapid degradation of an unstable enzyme is thereby excluded. Absence of radioactive labeling of terminal cells—erythrocytes, granulocytes, and platelets—is attributed to an insufficiency of the cosubstrate, phosphoribosepyrophosphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00485715
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