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  • Artikel  (56)
  • Springer  (28)
  • Springer Nature  (25)
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  • 1
    Digitale Medien
    Digitale Medien
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 226-236 
    ISSN: 1399-0047
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: In earlier sodium dodecylsulfate–polyacylamide gel electrophoresis (SDS–PAGE) studies it has been found that commonly utilized commercial hen egg-white lysozyme (HEWL) preparations contained 0.2–0.4 mol% covalently bound dimers. Here it is shown, using high-performance capillary electrophoresis (HPCE), that HEWL contains, in addition, two differently charged monomers in comparable amounts. To explore the origin of these microheterogeneous contaminants, purified HEWL (PHEWL) has been oxidized with hydrogen peroxide (0.0026–0.88 M) at various pH levels between 4.5 and 12.0. Optical densitometry of oxidized PHEWL (OHEWL) bands in SDS–PAGE gels shows that hydrogen peroxide at 0.88 M in acetate buffer pH 4.5 increased the amount of dimers about sixfold over that in commercial HEWL. OHEWL had, in addition to one of the two monomer forms found in HEWL and PHEWL, three other differently charged monomer forms, each of them representing about 25% of the preparation. SDS–PAGE analysis of OHEWL yielded two closely spaced dimer bands with Mr = 28 000 and 27 500. In addition, larger HEWL oligomers with Mr = 1.7 million and 320 000 were detected by gel-filtration fast protein liquid chromatography with multiangle laser light scattering detection. Non-dissociating PAGE in large pore size gels at pH 4.5 confirmed the presence of these large oligomers in HEWL and OHEWL. Increased microheterogeneity resulted in substantial effects on crystal growth and nucleation rate. On addition of 10 µg−1 mg ml−1 OHEWL to 32 mg ml−1 HEWL crystallizing solutions, both the number and size of forming crystals decreased roughly proportionally to the concentration of the added microheterogeneity. The same effect was observed in HEWL solutions on addition of 0.03–0.3 M hydrogen peroxide. Repartitioning of the dimer during crystallization at various temperatures between 277 and 293 K was analyzed by SDS–PAGE. The crystals contained ≤ 25%(w/w) of the oligomers in the solution, with no apparent temperature dependence of the repartitioning.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 776-784 
    ISSN: 1399-0047
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: Hen egg-white lysozyme (HEWL) is widely used as a model protein, although its purity has not been adequately characterized by modern biochemical techniques. We have identified and quantified the protein heterogeneities in three commercial HEWL preparations by sodium dodecyl sulfate polyacrylamide gel electrophoresis with enhanced silver staining, reversed-phase fast protein liquid chromatography (FPLC) and immunoblotting with comparison to authentic protein standards. Depending on the source, the contaminating proteins totalled 1–6%(w/w) and consisted of ovotransferrin, ovalbumin, HEWL dimers, and polypeptides with approximate Mr of 39 and 18 kDa. Furthermore, we have obtained gram quantities of electrophoretically homogeneous [〉 99.9%(w/w)] HEWL by single-step semi-preparative scale cation-exchange FPLC with a yield of about 50%. Parallel studies of crystal growth kinetics, salt repartitioning and crystal perfection with this highly purified material showed fourfold increases in the growth-step velocities and significant enhancement in the structural homogeneity of HEWL crystals.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 785-798 
    ISSN: 1399-0047
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Notizen: Nonuniform precipitant and impurity incorporation in protein crystals can cause lattice strain and, thus, possibly decrease the X-ray diffraction resolution. To address this issue, a series of crystallization experiments were carried out, in which initial supersaturation, NaCl concentration, protein purity level and crystallized fraction were varied. Lysozyme and protein impurities, as well as sodium and chloride were independently determined in the initial solution, supernatant and crystals. The segregation coefficients for Na+ and Cl− were found to be independent of supersaturation and NaCl concentration, and decreased with crystallized fraction/crystal size. Numerical evaluation of the extensive body of data, based on a nucleation-growth-repartitioning model, suggests a core of \sim40 µm in which salt is incorporated in much greater concentrations than during later growth. Small crystals containing higher amounts of incorporated NaCl also had higher protein impurity contents. This suggests that the excess salt is associated with the protein impurities in the core. X-ray topography revealed strain fields in the center of the crystals comparable in size to the inferred core. The growth rates of crystals smaller than 30–40 µm in size were consistently 1.5–2 times lower than those of larger crystals, presumably due to higher chemical potentials in the core.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Marine geophysical researches 13 (1991), S. 287-309 
    ISSN: 1573-0581
    Schlagwort(e): SeaMARC II ; side-scan ; bathymetry ; magnetics microplates ; rift propagation ; East Pacific Rise
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract The Pito Rift area is the site of actively deforming oceanic lithosphere that has been primarily under extension for at least the past million years, based on kinematic reconstructions. The major morphologic features, Pito Deep and Pito Seamount, are aligned toward the Euler pole for relative motion between the Easter and Nazca plates. SeaMARC II side-scan and bathymetry data indicate that there are two general modes of faulting currently active in the Pito Rift area. One is associated with incipient rifting of old (∼3 Ma) Nazca lithosphere by large NW-SE normal faults, and the other is associated with a broad area of right-lateral transform shear between the Nazca and Easter plates. This transform shear is distributed over a broad region because of the northward growth of the East Rift and parallel tectonic rifting within the Pito Rift area. The majority of the Pito Rift area is composed of preexisting blocks of Nazca plate that are back-tilted away from Pito Deep and strike perpendicular to present and previous relative plate motions. This observation suggests that block-faulting and back-tilting are the primary mechanisms responsible for the distributed lithospheric extension, in agreement with gravity and magnetic analyses (Martinez et al., this issue). The only recent volcanic flows observed in side-scan data are from the Pito Seamount area and to the outside of the outer pseudofault of the East Rift. The significance of the young flows near the outer pseudofault is not understood. We interpret the flows extending northwest from the Pito Seamount as representing a newly formed seafloor spreading axis within the Pito Rift area. Gravity and magnetic analyses (Martinez et al., this issue) together with SeaMARC II bathymetry and side-scan data support this interpretation. Based on the tectonic evolution of the Easter microplate, we propose an evolutionary model for the formation of the Pito Rift area, where new ‘tectonic’ grabens form immediately west of the previous graben and with slightly more counterclockwise orientation. The duration and history of tectonic activity for each graben are not well constrained.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    ISSN: 1573-0581
    Schlagwort(e): Magnetic inversion ; Bouguer anomalies ; block faulting ; modelling ; microplate ; rift propagation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract We present results from a SeaMARC II bathymetry, gravity, and magnetics survey of the northern end of the large-offset propagating East Rift of the Easter microplate. The East Rift is offset by more than 300 km from the East Pacific Rise and its northern end has rifted into approximately 3 Ma lithosphere of the Nazca Plate forming a broad (70–100 km) zone of high (up to 4 km) relief referred to as the Pito Rift. This region appears to have undergone distributed and asymmetric extension that has been primarily accommodated tectonically, by block faulting and tilting, and to a lesser degree by seafloor spreading on a more recently developed magmatic accretionary axis. The larger fault blocks have dimensions of 10–15 km and have up to several km of throw between adjacent blocks suggesting that isostatic adjustments occur on the scale of the individual blocks. Three-dimensional terrain corrected Bouguer anomalies, a three-dimensional magnetic inversion, and SeaMARC II backscatter data locate the recently developed magmatic axis in an asymmetric position in the western part of the rift. The zone of magmatic accretion is characterized by an axis of negative Bouguer gravity anomalies, a band of positive magnetizations, and a high amplitude magnetization zone locating its tip approximately 10 km south of the Pito Deep, the deepest point in the rift area. Positive Bouguer gravity anomalies and negative magnetizations characterize the faulted area to the east of the spreading axis supporting the interpretation that this area consists primarily of pre-existing Nazca plate that has been block faulted and stretched, and that no substantial new accretion has occurred there. The wide zone of deformation in the Pito Rift area and the changing trend of the fault blocks from nearly N-S in the east to NW-SE in the west may be a result of the rapidly changing kinematics of the Easter microplate and/or may result from ridge-transform like shear stresses developed at the termination of the East Rift against the Nazca plate. The broad zone of deformation developed at the Pito Rift and its apparent continuation some distance south along the East Rift has important implications for microplate mechanics and kinematic reconstructions since it suggests that initial microplate boundaries may consist in part of broad zones of deformation characterized by the formation of lithospheric scale fault blocks, and that what appear to be pseudofaults may actually be the outer boundaries of tectonized zones enclosing significant amounts of stretched pre-existing lithosphere.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Applied physics 54 (1992), S. 293-299 
    ISSN: 1432-0630
    Schlagwort(e): 68.55 ; 73.60F ; 78.65J ; 81.15E
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract Stoichiometric polycrystalline In2Se3 thin films have been grown by elemental evaporation on both glass and quartz substrates. The compositions are examined by DAN fluorimetry and X-ray photoelectron spectroscopy (XPS). Structure of the films are characterized by X-ray diffraction. The structure of this α-form of thin films have been determined to be hexagonal. Optimization of the preparative conditions employed for elemental evaporation, helped in preparing monophasic films by the suppression of other phases to a very minor extent. Influence of annealing conditions on the stoichiometry of the films are investigated in detail.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Biological cybernetics 70 (1994), S. 397-405 
    ISSN: 1432-0770
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Informatik , Physik
    Notizen: Abstract. An important step in visual processing is the segregation of objects in a visual scene from one another and from the embedding background. According to current theories of visual neuroscience, the different features of a particular object are represented by cells which are spatially distributed across multiple visual areas in the brain. The segregation of an object therefore requires the unique identification and integration of the pertaining cells which have to be “bound” into one assembly coding for the object in question. Several authors have suggested that such a binding of cells could be achieved by the selective synchronization of temporally structured responses of the neurons activated by features of the same stimulus. This concept has recently gained support by the observation of stimulus-dependent oscillatory activity in the visual system of the cat, pigeon and monkey. Furthermore, experimental evidence has been found for the formation and segregation of synchronously active cell assemblies representing different stimuli in the visual field. In this study, we investigate temporally structured activity in networks with single and multiple feature domains. As a first step, we examine the formation and segregation of cell assemblies by synchronizing and desynchronizing connections within a single feature module. We then demonstrate that distributed assemblies can be appropriately bound in a network comprising three modules selective for stimulus disparity, orientation and colour, respectively. In this context, we address the principal problem of segregating assemblies representing spatially overlapping stimuli in a distributed architecture. Using synchronizing as well as desynchronizing mechanisms, our simulations demonstrate that the binding problem can be solved by temporally correlated responses of cells which are distributed across multiple feature modules.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Biological cybernetics 66 (1992), S. 381-387 
    ISSN: 1432-0770
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Informatik , Physik
    Notizen: Abstract We present a scheme for systematically reducing the number of differential equations required for biophysically realistic neuron models. The techniques are general, are designed to be applicable to a large set of such models and retain in the reduced system as high a degree of fidelity to the original system as possible. As examples, we provide reductions of the Hodgkin-Huxley system and the A-current model of Connor et al. (1977).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Biological cybernetics 68 (1993), S. 209-214 
    ISSN: 1432-0770
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Informatik , Physik
    Notizen: Abstract We investigate spike initiation and propagation in a model axon that has a slow regenerative conductance as well as the usual Hodgkin-Huxley type sodium and potassium conductances. We study the role of slow conductance in producing repetitive firing, compute the dispersion relation for an axon with an additional slow conductance, and show that under appropriate conditions such an axon can produce a traveling zone of secondary spike initiation. This study illustrates some of the complex dynamics shown by excitable membranes with fast and slow conductances.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Biological cybernetics 70 (1994), S. 397-405 
    ISSN: 1432-0770
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Informatik , Physik
    Notizen: Abstract An important step in visual processing is the segregation of objects in a visual scene from one another and from the embedding background. According to current theories of visual neuroscience, the different features of a particular object are represented by cells which are spatially distributed across multiple visual areas in the brain. The segregation of an object therefore requires the unique identification and integration of the pertaining cells which have to be “bound” into one assembly coding for the object in question. Several authors have suggested that such a binding of cells could be achieved by the selective synchronization of temporally structured responses of the neurons activated by features of the same stimulus. This concept has recently gained support by the observation of stimulus-dependent oscillatory activity in the visual system of the cat, pigeon and monkey. Furthermore, experimental evidence has been found for the formation and segregation of synchronously active cell assemblies representing different stimuli in the visual field. In this study, we investigate temporally structured activity in networks with single and multiple feature domains. As a first step, we examine the formation and segregation of cell assemblies by synchronizing and desynchronizing connections within a single feature module. We then demonstrate that distributed assemblies can be appropriately bound in a network comprising three modules selective for stimulus disparity, orientation and colour, respectively. In this context, we address the principal problem of segregating assemblies representing spatially overlapping stimuli in a distributed architecture. Using synchronizing as well as desynchronizing mechanisms, our simulations demonstrate that the binding problem can be solved by temporally correlated responses of cells which are distributed across multiple feature modules.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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