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  • 1
    Electronic Resource
    Electronic Resource
    Chromosomal DNA transfer in Mycobacterium smegmatis is mechanistically different from classical Hfr chromosomal DNA transfer (2005)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 58 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Classical conjugal DNA transfer of chromosomal DNA in bacteria requires the presence of a cis-acting site, oriT, in the chromosome. Acquisition of an oriT occurs if a conjugative plasmid integrates into the chromosome to form an Hfr donor strain, which can transfer extensive regions of chromosomal DNA. Because oriT sequences are unique, and because transfer occurs in a 5′ to 3′ direction, the frequency with which a particular gene is inherited by the recipient depends on the gene's location: those closest to the 3′ side of oriT are transferred most efficiently. In addition, as the entire chromosome must be transferred to regenerate oriT, Hfr transconjugants never become donors. Here we describe novel aspects of a chromosomal DNA transfer system in Mycobacterium smegmatis. We demonstrate that there are multiple transfer initiations from a donor chromosome and, as a result, the inheritance of any gene is location-independent. Transfer is not contiguous; instead, multiple non-linked segments of DNA can be inherited in a recipient. However, we show that, with appropriate selection, segments of DNA at least 266 kb in length can be transferred. In further contrast to Hfr transfer, transconjugants can become donors, suggesting that the recipient chromosome contains multiple cis-acting sequences required for transfer, but lacks the trans-acting transfer functions. We exploit these observations to map a donor-determining locus in the M. smegmatis chromosome using genetic linkage analysis. Together, these studies further underline the unique nature of the M. smegmatis chromosomal transfer system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2005.04824.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genome-wide transcription analyses in rice using tiling microarrays (2006)
    [s.l.] : Nature Publishing Group
    Nature genetics 38 (2006), S. 124-129 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Sequencing and computational annotation revealed several features, including high gene numbers, unusual composition of the predicted genes and a large number of genes lacking homology to known genes, that distinguish the rice (Oryza sativa) genome from that of other fully sequenced model species. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1704
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  • 3
    Electronic Resource
    Electronic Resource
    Optical Properties of Hydrothermally Synthesized Hematite Particulate Pigments (2005)
    Oxford, UK : Blackwell Science Inc
    Journal of the American Ceramic Society 88 (2005), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Iron oxide hematite particles with various shapes (platelet, polyhedron, pseudocube, and peanut-like) have been synthesized by hydrothermal treatment of a Fe(OH)xOy precursor under various conditions. The size and shape of hematite particles can be adjusted by carefully controlling the processing parameters such as holding time, temperature, and adsorption ions present in the system. The nearly monosized α-Fe2O3 platelets possess face diameters of approximately 3 μm and a thickness of 0.5 μm under a scanning electron microscope. The apparent color of the particles changes as particle size and shape varies. Munsell color notation was employed to compare the color of hematite particles with various sizes and shapes. Diffuse reflectance spectra show that a “red-shift” of 40 nm is observed in platelet, pseudocube, and peanut-like particles compared with conventional particles. The band at 850 nm for the 6A1→4T1 transition was split in the pseudocubic and peanut-like particles. Raman spectra of the hematite particles also reveal that the vibrational modes of α-Fe2O3 particles diminish as particle size decreases, and dependence of vibrational band intensity on frequency is also observed. The spectral profiles demonstrate significant difference as excitation radiation lines changes from blue (457 nm) to red (647 nm). Possible mechanisms responsible for the optical properties of hematite particles are postulated based on the findings of the experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1551-2916.2005.00643.x
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  • 4
    Electronic Resource
    Electronic Resource
    The Toughening Action of Comprehensive Transformation on Mixed Mode II-III Ceramics (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 280-283 (Feb. 2007), p. 1779-1782 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: By the use of comprehensive transformation criterion and the method of weight function, the fracture enhancement of mixed-mode II-III crack in transformation toughened ceramics is predicated. The theoretical expressions of the toughening effect for both the stationary and steady-state growing crack are given respectively. The results show there is no toughening effect for the stationary crack and the toughening effect for the steady-state growing crack is associated with themodulus of elasticity, the width of transformation and its volume fraction
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.280-283.1779.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of Isothermal Holding Temperature on NGS Kinetics of Phosphorus and Temper Embrittlement (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 348-349 (Sept. 2007), p. 549-552 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: When failure occurs in material, it is often occurs by fracture along some grain boundariesand often by the micro-segregation of embrittling impurity to the grain boundaries. In the presentwork, the non-equilibrium grain-boundary segregation (NGS) kinetics of phosphorus and the temperembrittlement at the same solution treatment and different isothermal holding temperature in steel2.25Cr1Mo are studied. The NGS kinetics curves of phosphorus at the same solution temperature（1050 oC ）and different isothermal holding temperature (540 oC and 600 oC) are given. Experimentalresults provide a direct evidence of NGS kinetic model and show that the grain boundary segregationconcentrations of phosphorus for specimen isothermal holding at 540 oC are higher than those at 600oC. The peak values of AES patterns of solute atoms for specimen isothermal holding at 540 oC arealso higher than those at 600 oC. It is therefore concluded that the lower the isothermal holdingtemperature, the higher the segregation concentration of phosphorus at the grain-boundaries, and alsothe higher the degree of embrittlement
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/55/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.348-349.549.pdf
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  • 6
    Electronic Resource
    Electronic Resource
    Expression of ODF and ICAM-1 of Bone Marrow Mesenchymal Stem Cells is Enhanced with Osteogenic Differentiation (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 361-363 (Nov. 2007), p. 1173-1176 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Osteoblasts were perceived as pivotal cells, recognized as the cells that control both the formativeand the resorptive phases of the bone remodeling cycle. Osteoblasts were an essential requirement forosteoclastogenesis though expressing or secreating bioactive osteoclast-differentiation-regulatory proteins,osteoclast differentiation factor (ODF)was the most important factor among these, ODF participate nearly inevery step of differentiation and activation of osteoclasts. In addition, intercellular adhesion molecule-1(ICAM-1)and its receptors LFA-1 play a role in osteoclast development by affecting adhesion betweenstromal cells and osteoclast progenitors before the occurrence of ODF-ODF receptor signaling. However, itis not clear about the relationship between ODF, ICAM-1 expression of osteoblasts and differentiation stateof osteoblasts. So,the aim of this study was to investgate whether the expression of ODF, ICAM-1 dependedon the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells(rBMSCs). Theviability of rBMSCs is reduced significantly by osteogenic inducement as differentiating into osteoblasts,ALPase activity of OS-treated rBMSCs was enhanced obviously within 9 days , declined subsequently andrecovered nearly the original level at day 14. Expression of ODF is enhanced with osteogenic differentiationguadully. whereas, expression of ICAM-1 is activated at OS-treated day 6, then keeping at a stable level.This study indicated that rBMSCs undergoing osteogenic inducement was an ideal model for studying thedifferentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts fromstem cells to osteocytes, rBMSCs or Osteoprogenitor react somewhat differently from osteoblasts,suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have beenimproved with osteogenic culture
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1173.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of Heat Treatment on Specific Surface Area of Si-C-O Fibers (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 368-372 (Feb. 2008), p. 1639-1641 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this paper, effect of heat treatment on the SSA of Si-C-O fibers was investigated andmorphologies of the treated fibers were studied using SEM. The results revealed that weight loss wasproportional to the treatment time at 1573K and the specific surface area (SSA) increased sharply whenthe weight loss reached above 6wt%. A rough and porous ceramic fiber with SSA of 23.76m2/g could beobtained at the weight loss of 9.1wt%, as a result of the treatment at 1573K for 32h
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.368-372.1639.pdf
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  • 8
    Electronic Resource
    Electronic Resource
    The Synthesis and Phase Behavior of a Novel Discoticnematic Triphenylene Mesogen and its Application to an Optically Anisotropic Film (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 373-374 (Mar. 2008), p. 678-681 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A novel discotic triphenylene derivative bearing one triphenylene discogen as core andthree cyano-biphenyl moieties was synthesized and its thermal behavior was studied using polarizingoptical microscope, differential scanning calorimeter and X-ray diffraction. It was found that thismolecule containing [decylene?] spacers showed nematic phase. After being mixed with methylmethacrylate (MMA), the discogen showed nematic phase at the mesophase temperature range(64~85 ºC) and oriented spontaneously in the mixture after annealing for 2 hours in the mixture. Theorientation can be stabilized by the polymerization of MMA. The orientation can be fixed by thepolymerization of MMA. It was treated directly on glass substrate, which surface did not treat bypolyimide film and confirmed the orientation was proved by X-ray diffraction. This offers If thealignment of the discotic molecules can be freely controlled and fixed, a useful mean to produce newarea of optically anisotropic film will be produced with this material
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.373-374.678.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of Nozzle Type and Abrasive on Machinablity in Micro Abrasive Air Jet Machining of Glass (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 359-360 (Nov. 2007), p. 404-408 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Micro abrasive jet machining (MAJM) is a high effective economical technology for threedimensional micro structure fabrications of brittle materials. It is widely used in the fabrication ofsemiconductors, electronic devices, micro electro-mechanical systems (MEMS), optical-electronicand optical devices, and medical instruments. In this paper, based on the experimental study ofMAJM for glass, the influences of the nozzle type/size and abrasive type on the material erosion rateand structure profile are analyzed. The results show that the rectangular nozzle can yield a moreaccurate and efficient machining performance due to reduced flux effect. By contrast, with roundnozzle all the cross section profiles of the machined patterns show rounded V-type shape. Therectangular nozzle is able to produce geometry with flat shape. The machining area is almostindependent of the abrasive hardness, and the erosion rate increases with the increase in the hardnessof the abrasive grits. The nozzle geometry is of great importance for machining accuracy and theefficiency
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/55/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.359-360.404.pdf
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  • 10
    Electronic Resource
    Electronic Resource
    Corrosion Behavior of TiO〈sub〉2〈/sub〉 Coating on Magnesium Alloy AM60 in Hank’s Solution (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 373-374 (Mar. 2008), p. 609-612 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: As magnesium is an essential element for the human body, magnesium alloys areexpected to be promising implant materials despite their lower corrosion resistance. The mostpotential advantages of magnesium lie on both excellent biocompatibility and good mechanicalproperties. A TiO2 coating had been successfully deposited on the surface of AM60 samples byusing a thermal spray technique and was then sealed with sodium silicate. The corrosionbehavior was investigated by using the potentiodynamic electrochemical technique. Theexperimental results demonstrated that the TiO2 coating had a porous ceramic structure. Thethickness and micro hardness of the layer reached 40 μm and 886 HV, respectively. Afteradditional sealing with sodium silicate, TiO2 coating on magnesium alloy improved corrosionresistance in Hank’s solution
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.373-374.609.pdf
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