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  • Springer  (147)
  • 2020-2022  (66)
  • 2015-2019  (70)
  • 2000-2004  (2)
  • 1995-1999  (3)
  • 1985-1989  (4)
  • 1925-1929  (2)
  • 1910-1914
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  • 1
    Electronic Resource
    Electronic Resource
    Population fluctuations of the western tent caterpillar in southwestern British Columbia (2000)
    Springer
    Population ecology 42 (2000), S. 231-241 
    Details
    ISSN: 1437-5613
    Keywords: Key words Population cycles ; Nucleopolyhedrovirus ; NPV ; Insect quality ; Metapopulation ; Island populations ; Forest tent caterpillars ; Sublethal disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclic populations of western tent caterpillars fluctuate with a periodicity of 6–11 years in southwestern British Columbia, Canada. Typically, larval survival is high in early stages of the population increase, begins to decline midway through the increase phase, and is low through several generations of the population decline. Fecundity is generally high in increasing and in peak populations but is also reduced during the population decline. Poor survival and low fecundity for several generations cause the lag in recovery of populations that is necessary for cyclic dynamics. The dynamics of tent caterpillar populations vary among sites, which suggests a metapopulation structure; island populations in the rainshadow of Vancouver Island have more consistent cyclic dynamics than mainland populations in British Columbia. Sudden outbreaks of populations that last a single year suggest that dispersal from source to sink populations may occur late in the phase of population increase. Wellington earlier discussed qualitative variation among tent caterpillar individuals as an aspect of population fluctuations. The variation in caterpillar activity he observed was largely statistically nonsignificant. Recent observations show that the frequency of elongate tents as described by Wellington to characterize active caterpillars varies among populations but does not change in a consistent pattern with population density. The level of infection from nucleopolyhedrovirus (NPV) was high in some populations at peak density but was not associated with all population declines. Sublethal infection can reduce the fecundity of surviving moths, and there is a weak association between viral infection and egg mass size in field populations. The impact of weather in synchronizing or desynchronizing populations is a factor to be investigated further.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012002
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  • 2
    Electronic Resource
    Electronic Resource
    Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time (1999)
    Springer
    Plant cell reports 19 (1999), S. 32-36 
    Details
    ISSN: 1432-203X
    Keywords: Key words Allium sativum ; Benzyladenine ; Picloram ; Thidiazuron ; 2 ; 4-D ; 2iP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the same basal medium with 4.7 μM picloram+0.49 μM 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 μM picloram+13.3 μM BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050706
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  • 3
    Electronic Resource
    Electronic Resource
    Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets (1998)
    Springer
    Plant cell reports 17 (1998), S. 726-730 
    Details
    ISSN: 1432-203X
    Keywords: Key words Garlic ; Allium sativum L. ; Root segments ; Regeneration ; Picloram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 4,5 μm 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μm 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μm 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050473
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  • 4
    Electronic Resource
    Electronic Resource
    Bypassing prefertilization barriers to hybridization in Nicotiana using in vitro pollination and fertilization (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 665-671 
    Details
    ISSN: 1432-2242
    Keywords: Wide hybridization ; Tissue culture ; Ovary culture ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro pollination of placenta attached ovules was useful in bypassing unilateral incongruity barriers for several Nicotiana interspecific hybrid combinations (N. tabacum cv. ‘Ky 17’ X N. amplexicaulis, ‘Ky 17’ X N. benthamiana, and ‘Ky 17’ X N. repanda). By measuring the pollen tube growth over time, prefertilization barriers were determined to be the cause of the incongruity. Seedling necrosis was a problem in the development of the N. amplexicaulis hybrid and it prevented maturation of the N. repanda hybrid. Callus produced from cotyledons of the N. amplexicaulis hybrid eventually resulted in plants that survived to maturity. This procedure was not successful for the N. repanda materials. The N. amplexicaulis and N. benthamiana hybrids were sterile but following chromosome doubling by midrib culture, male and female fertile plants were produced. Conventional hybridization, fertilized ovule culture, and in vitro pollination were unsuccessful in obtaining hybrids of ‘Ky 17’ crossed with N. arentsii or N. bonariensis. Apparently, strong postfertilization barriers prevent the production of viable seed of these hybrids. Each of the N. repanda — N. tabacum reciprocal hybrids could not be rescued using callus culture; this adds support to the existence of strong sexual postfertilization barriers. A recent report, however, showed that it was possible to obtain this hybrid using the technique of somatic hybridization. Thus, it appears that it may also be possible to obtain asexual hybrids of N. arentsii and N. bonariensis with N. tabacum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260773
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  • 5
    Electronic Resource
    Electronic Resource
    Induction of diploid androgenetic and mitotic gynogenetic Nile tilapia (Oreochromis niloticus L.) (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 205-210 
    Details
    ISSN: 1432-2242
    Keywords: Androgenesis ; Gynogenesis ; Tilapia Gene bank ; Chromosome manipulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Androgenesis is a potentially valuable technique for recovering fish from gene banks composed of cryopreserved sperm, developing inbred lines, and analyzing patterns of inheritance. The procedure for producing diploid organisms whose nuclear DNA is wholly of paternal origin is dependent on: (1) the denucleation of “host” eggs, and (2) the inhibition of the first mitotic division in order to double the haploid sperm chromosome complement following fertilization of host eggs. Denucleation of tilapia (Oreochromis niloticus L.) eggs was carried out using UV irradiation. Treatment durations of 5–8 min (total dose of 450–720 J/m2) produced acceptable yields of viable denucleated eggs [22.9±1.6% (±SE) of controls] as estimated by the survival of haploid androgenetic tilapia to 48 h post-fertilization. Successful mitotic inhibition was accomplished using a heat-shock of 42.5 °C for 3–4 min, applied at 2.5-min intervals from 22.5 to 30 min post-fertilization (mpf). The mean survival of androgenetic diploid fish to yolk-sac absorption for treatment groups varied from 0.4% to 5.3%, relative to the controls. Differences in the suceptibility of eggs from different females to UV irradiation were a significant factor in the overall yield of androgenetic diploids. Paternal effects did not significantly influence the androgenetic yield, suggesting that individual males would not be selected against. For comparative purposes mitotic gynogenetic “mitogyne” diploids were produced from UV-irradiated sperm. Mean survival to yolk-sac absorption varied from 0.5% to 10.64%, relative to controls. Similar optima for androgenetic and gynogenetic induction were found in the period 25–27.5 mpf (minutes post-fertilization). Induction treatments would appear to be operating on the same developmental events in both these techniques, and the results suggest that the UV irradiations used do relatively little damage to the eggs beyond nuclear inactivation. The results indicate that the production of androgenetic O. niloticus is possible on a consistent basis and that the application of this technique may be useful in quantitative and conservation genetics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222203
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro regeneration in Trifolium. 1. Direct somatic embryogenesis in T. rubens (L.) (1988)
    Springer
    Plant cell, tissue and organ culture 15 (1988), S. 33-45 
    Details
    ISSN: 1573-5044
    Keywords: somatic embryogenesis ; tissue culture ; histology ; Trifolium ; zygotic embryogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The origin and development of zygotic and somatic embryos of Trifolium rubens L. was studied with the aid of paraffin sections and light microscopy. Zygotic embryos were collected, fixed and prepared daily from one to ten days after cross-pollination. Somatic embryos were obtained by plating petiole sections on modified L2 medium with 0.015 mgl-1 picloram and 0.1 mgl-1 6-BAP. Cultured petioles were collected and fixed daily from one to 25 days after plating. Two regions in the vascular bundle sheath of cultured petioles gave rise to callus. The first region was adjacent to the phloem fibers and produced friable callus. The second region gave rise to compact callus that was connected to the fascicular cambium. Somatic embryos originated from single cells in the cortex directly without intervening callus formation and from single cells in the friable callus. In addition, embryos arose from meristematic regions in compact callus. Many early stages of embryogenesis (one, two and four-celled stages) were observed in the cortex and friable callus. Zygotic embryogenesis in Trifolium differs from other legumes in that the suspensor is short and has a broad attachment. This arrangement was observed in zygotic embryos of T. rubens and in many somatic embryos. However, a continuum of somatic embryogenesis was observed where some young embryos had a Trifolium suspensor-like arrangement while others were attached to a long narrow suspensor-like structure more characteristic of Medicago.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039887
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  • 7
    Electronic Resource
    Electronic Resource
    Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.) (1989)
    Springer
    Plant cell, tissue and organ culture 19 (1989), S. 113-127 
    Details
    ISSN: 1573-5044
    Keywords: somatic embryogenesis ; plant regeneration ; protoplasts ; Trifolium pratense ; red clover ; protoclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts are useful for subcellular studies, in vitro selection, somatic hybridization and transformation. Whole plant regeneration from protoplasts is a prerequisite to producing altered crop plants using these methods. Whole plant regeneration was achieved from leaf- and suspension culture-derived protoplasts of T. pratense. Regeneration was most dependent upon identifying genotypes with genetic capacity to regenerate. Additional factors that were used to select genotypes, but which proved to be less important, were a high rate of cell growth in culture and a high plating efficiency of protoplasts. One genotype was identified which had a regeneration response equivalent to that of T. rubens and which regenerated from both leaf- and suspension culture-derived protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035811
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic male sterility in the pea (Pisum sativum L.): I. Inheritance, allelism and linkage (1988)
    Springer
    Euphytica 38 (1988), S. 165-174 
    Details
    ISSN: 1573-5060
    Keywords: Pisum sativum ; pea ; genetic male sterility ; genetics ; allelism ; linkage ; meiotic mutants ; microsporogenesis ; outcrossing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Relatively few male sterile (ms) genes have been reported in the pea despite description of numerous qualitative genes for other traits. This study was undertaken to characterize genetic male sterility in the pea. Male sterility may facilitate outcrossing because the pea is cleistogamous. Of fourteen lines carrying male sterility, tests of allelism revealed nine unique ms genes. According to F2 and F3 data, all ms genes segregated as single recessive genes. Linkage relations were determined for all but two. The genes appeared to be distributed randomly across the chromosomes. Two ms genes (ms-3 and ms-10) exhibited reduced female fertility in addition to male sterility. These mutants may have utility in increasing genetic recombination, particularly where hand hybridization is used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040188
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  • 9
    Electronic Resource
    Electronic Resource
    Destillation (1929)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 76 (1929), S. 367-369 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01380210
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  • 10
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    Performance of the missing transverse momentum triggers for the ATLAS detector during Run-2 data taking (2020)
    Springer
    Details
    Publication Date: 2020-08-01
    Print ISSN: 1126-6708
    Electronic ISSN: 1029-8479
    Topics: Physics
    Published by Springer
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