ISSN:
1573-0972
Keywords:
Invasion
;
IpaABCD
;
lipopolysaccharides
;
Plasmid pPR1347
;
Sereny
;
triparental cross
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A Shigella dysenteriae 1 strain isolated from an epidemic in West Bengal, India. The strain contained six plasmids including a large virulence plasmid. A plasmid, pPR1347 carrying both the rfb gene cluster and the rfc gene of Salmonella typhimurium has been transferred to this invasive Shigella dysenteriae 1 strain by triparental cross with a very low frequency. Only five stable (100%) clones were isolated after examining several thousand colonies. All five transconjugants were Sereny negative and were unable to invade the HeLa cells. Transconjugants exhibited strong cross reactivity with S. dysenteriae 1 antisera but they showed weak reaction with Salmonella typhimurium antisera. Plasmid profiles of the transconjugants were unaltered as compared with the wild type strain except for the presence of pPR 1347. The transconjugants regained their invasive property after elimination (curing) of pPR 1347. However, Shigella-infected convalescent phase serum was able to detect IpaABCD proteins from whole cell lysate and culture supernatant of transconjugants and cured (pPR 1347) transconjugants. A 60 kDa IpaH protein was not secreted into the culture supernatant by the transconjugants. Synthesis of lipopolysaccharides (LPS) of the hybrid strains was increased within the region of 43 to 67 kDa in comparison with the wild type S. dysenteriae 1 strains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008971915200
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