Publication Date:
1997-07-25
Description:
Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion between green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator binding sites integrated into the chromosome. Spindle microtubules were detected by expression of a protein fusion between GFP and Tub1, the major alpha tubulin. Spindle elongation and chromosome separation exhibited biphasic kinetics, and centromeres separated before telomeres. Budding yeast did not exhibit a conventional metaphase chromosome alignment but did show anaphase A, movement of the chromosomes to the poles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Straight, A F -- Marshall, W F -- Sedat, J W -- Murray, A W -- New York, N.Y. -- Science. 1997 Jul 25;277(5325):574-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, Box 0444, School of Medicine, University of California at San Francisco, San Francisco, CA 94143-0444, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9228009" target="_blank"〉PubMed〈/a〉
Keywords:
*Anaphase
;
Bacterial Proteins/metabolism
;
Centromere/chemistry/physiology
;
Chromatids/physiology
;
Chromosomes, Fungal/chemistry/*physiology
;
*Escherichia coli Proteins
;
Green Fluorescent Proteins
;
Lac Repressors
;
Luminescent Proteins
;
*Metaphase
;
Microscopy, Fluorescence
;
Microtubules/ultrastructure
;
*Mitosis
;
Movement
;
Operator Regions, Genetic
;
Recombinant Fusion Proteins
;
Repressor Proteins/metabolism
;
Saccharomyces cerevisiae/*cytology
;
Spindle Apparatus/physiology/ultrastructure
;
Telomere/physiology
;
Tubulin/analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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