ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

Improved Screening of cDNAs Generated by mRNA Differential Display Enables the Selection of True Positives and the Isolation of Weakly Expressed Messages (1997)

Zegzouti, Hicham ; Marty, Christel ; Jones, Brian ; [et al.]

Springer

Plant molecular biology reporter 15 (1997), S. 236-245

add to mindlist on the mindlist

Details

ISSN: 1572-9818

Keywords: Differential display ; Lycopersicon esculentum ; Ethylene-responsive ; Low abundance mRNAs

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The high percentage of false positives generated by differential display (as high as 85%) has previously limited the potential of the method. This report describes an efficient methodology that enables false positives to be discarded prior to cloning, via reverse Northern analysis. This first step of the screening also allows the detection of putative low abundance differential clones. Following cloning, a second reverse Northern combined with partial DNA sequencing and RT-PCR detection allows isolation of all differential cDNAs including very low abundance clones. Use of the sequential screening procedure described here led to the isolation of novel tomato genes responding to the plant hormone ethylene while minimising labor and materials input.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007482318668

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

2

Electronic Resource

Evaluation of ibuprofen versus aspirin and paracetamol on efficacy and comfort in children with fever (1997)

Autret, E. ; Reboul-Marty, J. ; Henry-Launois, B. ; [et al.]

Springer

European journal of clinical pharmacology 51 (1997), S. 367-371

add to mindlist on the mindlist

Details

ISSN: 1432-1041

Keywords: Key words Ibuprofen ; Fever ; Paracetamol ; Aspirin ; Child ; Comfort

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Medicine

Notes: Abstract Objective: We compared efficacy and impact on the comfort of ibuprofen (7.5 mg/kg per dose), aspirin (10 mg/kg/dose) and paracetamol (10 mg/kg per dose) on children with fever aged 6–24 months in an open, randomised study with three parallel groups. Methods: The main criterion for efficacy was area under the curve (AUC) of percentage temperature reduction. Comfort was assessed on scores depending on general behaviour and degree of relief. General behaviour was assessed on a verbal scale and on a visual analogue scale (VAS) and the degree of relief was assessed in relation to baseline on a verbal scale. Results: The efficacy of ibuprofen was better than that of aspirin or paracetamol. In spite of more adverse events, the comfort scores were significantly in favour of ibuprofen 6 h after the first dose of treatment.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002280050215

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

3

Electronic Resource

Dynamic orbit determination and gravity field model improvement from GPS, DORIS and Laser measurements on TOPEX/POSEIDON satellite (1997)

Perosanz, F. ; Marty, J. C. ; Balmino, G.

Springer

Journal of geodesy 71 (1997), S. 160-170

add to mindlist on the mindlist

Details

ISSN: 1432-1394

Source: Springer Online Journal Archives 1860-2000

Topics: Architecture, Civil Engineering, Surveying

Notes: Summary. In the framework of the GRIM series of gravity field models, the CNES/GRGS GINS precise orbit determination software has been adapted to dynamic GPS data processing. That is simultaneous processing of all available observables (i.e. GPS, DORIS, Laser) and all available satellite orbits (i.e. GPS, TOPEX/POSEIDON) can now be performed. The TOPEX/POSEIDON (T/P) mission satellite is equipped with a GPS receiver, a DORIS receiver and a laser reflector that represents an unprecedented opportunity to compare and combine these three tracking systems to estimate orbital parameters and gravity field coefficients. Different combinations including : GPS + DORIS, DORIS + LASER, GPS + DORIS + LASER, have been tested and have shown small but systematic improvement in T/P orbit accuracy when GPS and DORIS data were processed simultaneously. Five tuned gravity field models have been generated by accumulating different combinations of T/P normal equations associated to the GPS, DORIS and Laser data. GPS data have a greater dynamic impact on gravity field spherical harmonics coefficient determination than DORIS and Laser data. Furthermore, the results obtained with the solutions including and T/P normal equations suggest that indeed these different tracking systems are somehow complementary in a dynamic sense.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001900050084

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

4

Electronic Resource

Long-wavelength global gravity field models: GRIM4-S4, GRIM4-C4 (1997)

Schwintzer, P. ; Reigber, C. ; Bode, A. ; [et al.]

Springer

Journal of geodesy 71 (1997), S. 189-208

add to mindlist on the mindlist

Details

ISSN: 1432-1394

Source: Springer Online Journal Archives 1860-2000

Topics: Architecture, Civil Engineering, Surveying

Notes: Summary. GFZ Potsdam and GRGS Toulouse/Grasse jointly developed a new pair of global models of the Earth's gravity field to satisfy the requirements of the recent and future geodetic and altimeter satellite missions. A precise gravity model is a prerequisite for precise satellite orbit restitution, tracking station positioning and altimeter data reduction. According to different applications envisaged, the new model exists in two parallel versions: the first one being derived exclusively from satellite tracking data acquired on 34 satellites, the second one further incorporating satellite altimeter data over the oceans and terrestrial gravity data. The most recent “satellite-only” gravity model is labelled GRIM4-S4 and the “combined” gravity model GRIM4-C4. The models are solutions in spherical harmonics and have a resolution up to degree and order 60 plus a few resonance terms in the case of GRIM4-S4, and up to degree/order 72 in the case of GRIM4-C4, corresponding to a spatial resolution of 555 km at the Earth's surface. The gravitational coefficients were estimated in a rigorous least squares adjustment simultaneously with ocean tidal terms and tracking station position parameters, so that each gravity model is associated with a consistent ocean tide model and a terrestrial reference frame built up by over 300 optical, laser and Doppler tracking stations. Comprehensive quality tests with external data and models, and test arc computations over a wide range of satellites have demonstrated the state-of-the-art capabilities of both solutions in long-wavelength geoid representation and in precise orbit computation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001900050087

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

5

Electronic Resource

Mind Architecture and Brain Architecture (1997)

CELA-CONDE, CAMILO J. ; MARTY, GISÈLE

Springer

Biology and philosophy 12 (1997), S. 327-340

add to mindlist on the mindlist

Details

ISSN: 1572-8404

Keywords: mind architecture ; brain architecture ; computer metaphor ; chaos ; non-linear phenomena ; reductionism ; cognitive functionalism ; neurocomputation ; PDP ; knowledge process

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Philosophy

Notes: Abstract The use of the computer metaphor has led to the proposal of ’’mind architecture‘‘ (Pylyshyn 1984; Newell 1990) as a model of the organization of the mind. The dualist computational model, however, has, since the earliest days of psychological functionalism, required that the concepts ’’mind architecture‘‘ and ’’brain architecture‘‘ be remote from each other. The development of both connectionism and neurocomputational science, has sought to dispense with this dualism and provide general models of consciousness – a ’’uniform cognitive architecture‘‘ –, which is in general reductionist, but which retains the computer metaphor. This paper examines, in the first place, the concepts of mind architecture and brain architecture, in order to evaluate the syntheses which have recently been offered. It then moves on to show how modifications which have been made to classical functionalist mind architectures, with the aim of making them compatible with brain architectures, are unable to resolve some of the most serious problems of functionalism. Some suggestions are given as to why it is not possible to relate mind structures and brain structures by using neurocomputational approaches, and finally the question is raised of the validity of reductionism in a theory which sets out to unite mind and brain architectures.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006572424048

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

6

Electronic Resource

Periphyton distribution and abundance on substrata of different size along a gradient of stream trophy de Montréal (1997)

Cattaneo, Antonella ; Kerimian, Takie ; Roberge, Micheline ; [et al.]

Springer

Hydrobiologia 354 (1997), S. 101-110

add to mindlist on the mindlist

Details

ISSN: 1573-5117

Keywords: streams ; periphyton ; substratum size ; trophy

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract In stream, substrata of different size present different degree ofstability, current, erosion, and deposition to colonizingorganisms. In this study, we tested the importance of substratumsize ranging from sand to small boulders for periphytondistribution and abundance. Because trophy strongly affect streamorganisms, we sampled at nine sites chosen to represent the rangeof nutrients typical of Eastern Ontario and Western Québec. Alarge part of the variability in algal biomass (as chlorophyll)among sites was explained by trophy (as seston or totalphosphorus). However, there was also an effect of substratum size.Cobbles had the highest biomass, and gravel the lowest; sand andboulders were intermediate. Assemblages on different substrata weredifferent in taxonomy and life forms. Cyanobacterial colonies andmotile diatoms dominated the finer substrata while adnate andfilamentous algae were more developed on the larger ones.Consequently periphyton on fine sediments was more loosely attachedthan on rocks. Average algal size was not related to substratumsize but increased significantly with trophy confirmingobservations in benthic and planktonic assemblages inlakes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1003027927600

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

7

Electronic Resource

Er5, a tomato cDNA encoding an ethylene-responsive LEA-like protein: characterization and expression in response to drought, ABA and wounding (1997)

Zegzouti, Hicham ; Jones, Brian ; Marty, Christel ; [et al.]

Springer

Plant molecular biology 35 (1997), S. 847-854

add to mindlist on the mindlist

Details

ISSN: 1573-5028

Keywords: differential display ; drought stress ; ethylene inducible ; LEA protein ; Lycopersicon esculentum ; RT-PCR

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We report the isolation by differential display of a novel tomato ethylene-responsive cDNA, designated ER5. RT-PCR analysis of ER5 expression revealed an early (15 min) and transient induction by ethylene in tomato fruit, leaves and roots. ER5 mRNA accumulated during 2 h of ethylene treatment and thereafter underwent a dramatic decline leading to undetectable expression after 5 h of treatment. The full-length cDNA clone of 748 bp was obtained and DNA sequence analysis showed strong homologies to members of the atypical hydrophobic group of the LEA protein family. The predicted amino acid sequence shows 67%, 64%, 64%, and 61% sequence identity with the tomato Lemmi9, soybean D95-4, cotton Lea14-A, and resurrection plant pcC27-45 gene products, respectively. As with the other members of this group, ER5 encodes a predominantly hydrophobic protein. Prolonged drought stress stimulates ER5 expression in leaves and roots, while ABA induction of this ethylene-responsive clone is confined to the leaves. The use of 1-MCP, an inhibitor of ethylene action, indicates that the drought induction of ER5 is ethylene-mediated in tomato roots. Finally, wounding stimulates ER5 mRNA accumulation in leaves and roots. Among the Lea gene family this novel clone is the first to display an ethylene-regulated expression.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1005860302313

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

8

Electronic Resource

Micro-environment effect on the structure of synthetic substrates of thermolysin (1997)

Baltora-Rosset, Sylvie ; Marty, Danielle ; Dupradeau, François-Yves ; [et al.]

Springer

International journal of peptide research and therapeutics 4 (1997), S. 391-395

add to mindlist on the mindlist

Details

ISSN: 1573-3904

Keywords: Molecular modeling ; NMR ; Peptides

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary The conformations of thermolysin synthetic substrates in H2O/D2O (9/1) and glycerol-d 5 (5 M) are investigated using two-dimensional nuclear magnetic resonance spectroscopy and molecular modeling. The structures obtained from molecular modeling and NMR studies are compared. Comparisons of these structures with bound inhibitor in the active site of thermolysin are also discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02442905

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

9

Electronic Resource

Micro-environment effect on the structure of synthetic substrates of thermolysin (1997)

Baltora-Rosset, Sylvie ; Marty, Danielle ; Dupradeau, François-Yves ; [et al.]

Springer

International journal of peptide research and therapeutics 4 (1997), S. 391-395

add to mindlist on the mindlist

Details

ISSN: 1573-3904

Keywords: Molecular modeling ; NMR ; Peptides

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract The conformations of thermolysin synthetic substrates inH2O/D2O (9/1) and glycerol-d5 (5 M) are investigated using two-dimensional nuclearmagnetic resonance spectroscopy and molecular modeling. The structuresobtained from molecular modeling and NMR studies are compared. Comparisonsof these structures with bound inhibitor in the active site of thermolysinare also discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008881812479

Permalink