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Micro-environment effect on the structure of synthetic substrates of thermolysin

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Summary

The conformations of thermolysin synthetic substrates in H2O/D2O (9/1) and glycerol-d 5 (5 M) are investigated using two-dimensional nuclear magnetic resonance spectroscopy and molecular modeling. The structures obtained from molecular modeling and NMR studies are compared. Comparisons of these structures with bound inhibitor in the active site of thermolysin are also discussed.

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Authors and Affiliations

  1. Faculté de Pharmacie, Groupe de Recherche des Biomolécules: Micro-environnement et Métabolisme, I rue des Louvels, F-80037, Amiens Cedex 1, France

    Sylvie Baltora-Rosset, Danielle Marty, François-Yves Dupradeau, Gilles Gacel & Jean-Pierre Monti

  2. Department de Biologie, Université Cergy-Pontoise, F-95302, Cergy-Pontoise Cedex, France

    Emmanuel Pauthe & Véronique Larreta-Garde

Authors
  1. Sylvie Baltora-Rosset
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  2. Danielle Marty
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  3. François-Yves Dupradeau
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  4. Emmanuel Pauthe
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  5. Véronique Larreta-Garde
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  6. Gilles Gacel
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  7. Jean-Pierre Monti
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Baltora-Rosset, S., Marty, D., Dupradeau, FY. et al. Micro-environment effect on the structure of synthetic substrates of thermolysin. Lett Pept Sci 4, 391–395 (1997). https://doi.org/10.1007/BF02442905

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  • DOI: https://doi.org/10.1007/BF02442905

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