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1

Digitale Medien

Host-cell-specific glycosylation of HIV-2 envelope glycoprotein (1997)

Liedtke, Steffen ; Geyer, Rudolf ; Geyer, Hildegard

Springer

Glycoconjugate journal 14 (1997), S. 785-793

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ISSN: 1573-4986

Schlagwort(e): glycoprotein ; glycosylation ; gp120 ; HIV ; MALDI-TOF-MS

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Abstract Neutral complex-type N-glycans of the envelope glycoprotein 120 of HIV-2, propagated in different host cells, display cell-type specific variations. In order to identify typical structural elements, glycans were analysed by gel filtration, by enzymic sequencing and, in part, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The characteristic substituents of di- tri- and tetraantennary carbohydrate units thus observed include N-acetyllactosamine repeats, bisecting N-acetylglucosamine and fucose linked to the chitobiose core as well as to N-acetyllactosamine antennae. Each glycoprotein preparation displayed a characteristic set of glycoforms. Abbreviations: endo H, endo-β-N-acetylglucosaminidase H; E-PHA, Phaseolus vulgaris agglutinin E4; GlcNAcOH, N-acetyl-glucosaminitol; gp120/HUT78(MOLT4/Mφ/PBL/U937), external envelope glycoprotein 120 of HIV-2, strain D194, propagated in HUT78 (MOLT4, Mφ, PBL, U937) cells; gu, glucose units; HPAEC, high-pH anion-exchange chromatography; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Mφ, human monocytes/macrophages; PBL, human peripheral blood lymphocytes; PNGase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1018577619036

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2

Digitale Medien

Influence of management on butterflies of rare grassland ecosystems in Germany (1997)

Dolek, Matthias ; Geyer, Adi

Springer

Journal of insect conservation 1 (1997), S. 125-130

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ISSN: 1572-9753

Schlagwort(e): grazing ; mowing ; fens ; hay-meadows ; conservation.

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Wissenschaftskunde und Wissenschaftsorganisation, Hochschul- und Universitätswesen, Museumswissenschaft

Notizen: Abstract Traditional hay-meadows in the Alps and fens at the edge of the Alps are habitats for many rare and endangered butterfly species. Conservation efforts aim at preserving these species, but the biotopes depend on regular mowing, which in turn requires intensive financial support. The feasibility of substituting mowing of these sites by grazing is discussed and considered as a more cost effective management type which produces agriculturally valuable goods as well. In this study the butterfly fauna of mown and grazed sites were compared. Species composition, species number, and the occurrence of rare species under the two management types were in most cases rather similar for both grassland ecosystems. Nevertheless, there are hints that for single rare species this might not be true. Additionally, at one site, grazing intensity on a former hay-meadow was too high to preserve the species-rich community. Overall the results are encouraging: grazing does not have to be as detrimental as formerly thought, although details (compartments of pastures, intensity) still have to be confirmed. Experimental grazing management of aban-doned grasslands of the studied types should be started.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1018443412153

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3

Digitale Medien

Distribution of Maculinea rebeli and hostplant use on sites along the river Danube (1998)

Dolek, Matthias ; Geyer, Adi ; Bolz, Ralf

Springer

Journal of insect conservation 2 (1998), S. 85-89

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ISSN: 1572-9753

Schlagwort(e): butterflies ; Gentiana cruciata ; management ; grazing ; Bavaria.

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Wissenschaftskunde und Wissenschaftsorganisation, Hochschul- und Universitätswesen, Museumswissenschaft

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1009601024873

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4

Digitale Medien

Thermoanalytische Charakterisierung Strahlengehärteter Polymerfilme (1998)

Hösselbarth, B. ; Friese, K. ; Geyer, W.

Springer

Journal of thermal analysis and calorimetry 52 (1998), S. 145-155

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ISSN: 1572-8943

Schlagwort(e): acrylates ; DMA ; DSC ; epoxides ; radiation curing ; structure-property relationship ; TG ; thermal analysis

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Abstract Thermo-oxidative and thermo-mechanical stabilities of radiation-cured acrylates and epoxides were examined by TG, DMA and DSC. The polymeric trifunctional acrylates PETIA, TMPTA and THEIC displayed the highest temperatures of onset of degradation. The high crosslinking density of the films resulted in an almost temperature-independent complex E-modulus, as measured by DMA. With increasing degree of ethoxylation or propoxylation of the monomers, decreases in thermal stability and strength were found. For difunctional polymeric acrylates and epoxides, the glass transition temperature was measured. The average degree of curing of UV-cured epoxy films can be determined from the temperature of the maximum in the loss modulus (E″max.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1010122525663

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5

Digitale Medien

Expression of vascular endothelial growth factor during the development of cardiac hypertrophy in spontaneously hypertensive rats (1998)

McAinsh, Aileen M. ; Geyer, Marcel ; Fandrey, Joachim ; [weitere]

Springer

Molecular and cellular biochemistry 187 (1998), S. 141-146

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ISSN: 1573-4919

Schlagwort(e): VEGF ; hypertrophy ; spontaneously hypertensive rat ; coronary flow

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Abstract Left ventricular hypertrophy (LVH) is often associated with an impaired maximal coronary blood flow and increases the vulnerability of the heart tissue to ischaemia. In this study, the correlation between coronary blood flow and expression of the vascular endothelial growth factor (VEGF) mRNA was investigated. Using both haemodynamic measurements and analysis of mRNA, we have demonstrated that during development of LVH, in spontaneously hypertensive rats (SHR), an impaired maximal coronary flow at 12 weeks of age is associated with low levels of VEGF mRNA. However, in older SHR (32 weeks) with stabilised hypertrophy and a normal maximal coronary flow response, VEGF mRNA levels are increased three-fold. These results suggest that the mechanism for the impaired flow, observed in some types of cardiac hypertrophy, might involve an inadequate growth of the coronary vessels due to insufficient activation of the VEGF gene.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1006887510678

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6

Digitale Medien

Structural analysis of neutral glycosphingolipids from Ascaris suum adults (Nematoda: Ascaridida) (1997)

Lochnit, Gunter ; Dennis, Roger D ; Zahringer, Ulrich ; [weitere]

Springer

Glycoconjugate journal 14 (1997), S. 389-399

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ISSN: 1573-4986

Schlagwort(e): arthro-series glycosphingolipids ; Ascaris suum ; (iso-branched) C17 sphingoid-bases ; neutral glycosphingolipids ; parasitic nematodes

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Abstract The neutral glycosphingolipid fraction from adults of the pig parasitic nematode, Ascaris suum, was resolved into four components on thin-layer chromatography. The high-performance liquid chromatography-isolated components were structurally analysed by: methylation analysis; exoglycosidase cleavage; gas-liquid chromatography/mass spectrometry; liquid secondary-ion mass spectrometry; and, in particular, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Their chemical structures were determined as: Glc(β1-1)ceramide, Man(β1-4)Glc(β1-1)ceramide, GlcNAc(β1-3)Man(β1-4)Glc(β1-1)ceramide and Gal(α1-3)GalNAc(β1-4)GlcNAc(β1-3)Man(β1-4)Glc(β1-1)ceramide; and were characterized as belonging to the arthro-series of protostomial glycosphingolipids. No glycosphingolipid component corresponding to ceramide tetrasaccharide was detected during these analyses. The ceramide composition of the parent glycosphingolipids was dominated by the 2-(R)-hydroxy C24:0 fatty acid, cerebronic acid, and C17 sphingoid-bases: 15-methylhexadecasphing-4-enine and 15-methylhexadecaphinganine in approximately equal proportions. The component ceramide monohexoside was characterized by an additional 15-methylhexadecaphytosphingosine. Abbreviations: CDH, ceramide dihexoside; Cer, ceramide; CMH, ceramide monohexoside; CPH, ceramide pentahexoside; CTH, ceramide trihexoside; CTetH, ceramide tetrahexoside; Hex, hexose; HexNAc, N-acetylhexosamine; HPTLC, high-performance thin-layer chromatography; LSIMS, liquid secondary-ion mass spectrometry; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; N-, Nz- and A-glyco(sphingo)lipids, neutral, neutralzwitterionic and acidic glyco(sphingo)lipids, respectively

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1018530914067

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7

Digitale Medien

Slicing the Oppenheimer-Snyder Collapse: Harmonic versus Maximal Slicing (1997)

Geyer, Axel ; Herold, Heinz

Springer

General relativity and gravitation 29 (1997), S. 1257-1268

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ISSN: 1572-9532

Schlagwort(e): NUMERICAL RELATIVITY

Quelle: Springer Online Journal Archives 1860-2000

Thema: Physik

Notizen: Abstract In continuing our studies of temporal slicings of spacetimes, we consider the collapse from rest of an initially homogeneous dust sphere, known as Oppenheimer-Snyder collapse. It is shown that in the case of harmonic slicing the whole spacetime becomes covered by slices extending to spatial infinity in contrast to maximal slicing. The behavior of the lapse function is discussed, especially for various data on the initial slice, and it is demonstrated that not every choice of the initial lapse leads to meaningful slicings.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1018811829719

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8

Digitale Medien

Observation of antihydrogen production in flight at CERN (1997)

Baur, G. ; Boero, G. ; Brauksiepe, S. ; [weitere]

Springer

Hyperfine interactions 109 (1997), S. 191-203

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ISSN: 1572-9540

Quelle: Springer Online Journal Archives 1860-2000

Thema: Physik

Notizen: Abstract The observation of the production of antihydrogen atoms \overlineH 0\equiv\barpe+, the simplest atomic bound state of antimatter, is presented. A method has been used by the PS210 collaboration at LEAR which assumes that the production of \overlineH0 is predominantly mediated by the e+e--pair creation via the two-photon mechanism in the antiproton--nucleus interaction. Neutral \overlineH0 atoms are indentified by a unique sequence of characteristics. In principle \overlineH0 is well suited for investigations of fundamental CPT violation studies under different forces, however, in our investigations we concentrate on the production of this antimatter object, since so far it had not been observed. The production of eleven antihydrogen atoms is reported including possibly 2± 1 background signals, the observed yield agrees with theoretical predictions.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1012609601849

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