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  • Springer  (48)
  • Blackwell Science Ltd.
  • Geological Society of America (GSA)
  • 2015-2019
  • 1995-1999  (49)
  • 1950-1954
  • 1999  (18)
  • 1998  (17)
  • 1997  (14)
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  • 2015-2019
  • 1995-1999  (49)
  • 1950-1954
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  • 1
    ISSN: 1573-2657
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The main objective of this study was to analyse glycogen in single muscle fibres, using a recently developed microfluorometric method which detects subpicomol amounts of NADPH, glucose and glycogen (as glucosyl units) (detection limit 0.16–0.17pmol in a 25nl sample) without fluorochrome amplification. The fibres were freshly dissected from the twitch region of the iliofibularis muscle of the cane toad (Bufo marinus), and were mechanically skinned under paraffin oil to gain access to the intracellular compartments. The results show that: (1) glycogen concentrations in toad skeletal muscle fibres range between 25.8 and 369mmol glucosyl units/litre fibre volume; (2) there is a large variation in glycogen content between individual fibres from the iliofibularis muscle of one animal; (3) there are seasonal differences in the glycogen content of toad single muscle fibres; (4) the total amount of glycogen in single muscle fibres of the toad does not decrease significantly when storing the tissue, under paraffin oil, at 20–25°C for up to 6h or at 4°C for up to 24h; and (5) 15–26% of fibre glycogen can be washed in an aqueous solution at pH 5–7, within 5min, while 74–85% of fibre glycogen remains associated with the washed skinned fibre, even after 40min exposure of the skinned fibre preparation to the aqueous environment. The retention of most glycogen in the fibre preparation after mechanical removal of the plasma membrane and extensive washing indicates that in toad skeletal muscle fibres the largest proportion of glycogen is tightly bound to intracellular structures. The results also show that the skinned muscle fibre preparation is well suited for microfluorometric glycogen determination, since low molecular weight non-glycogen contributors to the fluorescence signal can be removed from the myoplasmic space prior to the glycogen hydrolysis step.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd.
    Journal of metamorphic geology 15 (1997), S. 0 
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Calcsilicate granulites of probable Middle Proterozoic age (c.1000–1100 Ma) in the vicinity of Battye Glacier, northern Prince Charles Mountains, East Antarctica, contain prograde metamorphic assemblages comprising various combinations of wollastonite, scapolite, clinopyroxene, An-rich plagioclase, calcite, quartz, titanite and, rarely, orthoclase, ilmenite, phlogopite and graphite. Comparison of the prograde assemblages with calculated and experimentally determined phase relations in the simple CaO–Al2O3–SiO2–CO2–H2O system suggests peak metamorphism at ≥835 °C in the presence (in wollastonite-bearing assemblages at least) of a CO2-bearing fluid (XCO≥0.3) at a probable pressure of 6–7 kbar.Well-preserved retrograde reaction textures represent: (1) breakdown of scapolite to anorthite+calcite±quartz; (2) formation of grossular–andradite garnet and, locally, (3) epidote, both principally by reactions involving scapolite breakdown products and clinopyroxene; (4) local coupled replacement of clinopyroxene and ilmenite by hornblende and titanite, respectively; and finally (5) local sericitization of prograde and retrograde plagioclase. These retrograde reactions are interpreted to be the result of cooling and variable infiltration by H2O-rich fluids, possibly derived from crystallizing pegmatitic intrusions and segregations that may be partial melts, which are common throughout the area.
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  • 3
    ISSN: 1435-604X
    Keywords: Keywords: Adenocarcinoma; Aminolaevulinic acid; Barrett's oesophagus; Dysplasia; Photodynamic therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract . Photodynamic therapy (PDT) may have a role in the prevention of oesophageal cancer. Ten patients with Barrett's oesophagus, three with low-grade dysplasia (LGD), four with high-grade dysplasia (HGD), one with carcinoma in situ and two with invasive carcinoma, were treated with PDT. All received 30 mg/kg aminolaevulinic acid (ALA) followed 4 h later by laser endoscopy. Half were treated with red light (630 nm; 100 mW/cm2 for 1000 s) and half with green light (514 nm; 100 mW/cm2 for 500 s). Columnar epithelial regression was seen in all patients with dysplasia (mean area decrease 44%; range 10–100%), with apparent elimination of dysplasia in all cases. In patients with in situ or invasive carcinoma, no response was seen. ALA-induced PDT, using either red or green light, produces effective ablation of dysplastic Barrett's oesophagus, hence may have a role in the prevention of oesophageal carcinoma, but has little effect on in situ or invasive adenocarcinoma.
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  • 4
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract. Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) were analyzed in storm water collected from 15 outfalls in San Francisco Bay, California, considered representative of areas located adjacent to petroleum refineries and mixed urban/commercial/residential land uses. Storm water sampling was conducted hourly over a 12-h period at two outfalls located near the city of Oakland and the suburban community of Benicia, which represent urban and mixed urban/light industrial land uses, respectively. Sampling was conducted hourly to determine temporal changes in the concentrations and distributions of PCDD/Fs during the first major rainstorm event of the 1995/96 winter season. In addition, storm water sampling was conducted in December 1995 and February/April 1996 at six publicly owned treatment work (POTW) outfalls located adjacent to developed, undeveloped, mixed residential/commercial, and industrial lands. Storm water sampling also was conducted at five outfalls located adjacent to petroleum refineries. The sampling results were used to examine changes in PCDD/F loadings to San Francisco Bay at the onset and midway through the winter rainy season and to characterize differences in PCDD/F concentrations, if any, between refinery and nonrefinery areas. The concentrations of tetra- through octa-chlorinated homologues and 2,3,7,8-substituted congeners were assayed in filtered storm water samples according to US EPA Method 1613A. Principal components analysis (PCA) was used as an exploratory technique to examine the similarities and differences in the distributions of PCDD/Fs in storm water from different outfalls. The sampling results showed few significant differences between storm water discharged from outfalls located in mixed urban/commercial/residential areas and outfalls located adjacent to petroleum refineries. The concentrations of 2,3,7,8-TCDD were below the analytical limits of detection in all storm water samples, with the exception of samples collected after the sixth hour of sampling at the Oakland outfall. Fingerprint patterns were generally dominated by the higher chlorinated PCDD/F congeners including OCDD, OCDF, and 1,2,3,4,6,7,8-HpCDD. Total TEQs were generally below US EPA ambient water quality criteria and aquatic ecotoxicology guideline values. The results of this study strongly suggest that discrete sampling of storm water outfalls is insufficient to characterize the concentrations and distributions of persistent hydrophobic contaminants such as PCDD/Fs. In addition to surface water runoff from the city of Oakland, other nonindustrialized urban locations may represent important sources of PCDD/Fs to San Francisco Bay.
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  • 5
    ISSN: 1432-0630
    Keywords: PACS: 68.55; 72.20; 81.10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 6
    ISSN: 1432-0495
    Keywords: Key words Natural potential technique ; Topographic effect ; Karst terrane ; Groundwater recharge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The natural (electrical) potential (NP) method – also known as self-potential, spontaneous potential and streaming potential (SP) – has been used to locate areas of groundwater flow in karst terrane. NP is the naturally occurring voltage at the ground surface resulting from ambient electrical currents within the earth. The measurement of NP can be used to characterize groundwater flow in karst terrane because electrical potential gradients are generated by the horizontal flow of water along fractures or conduits and the vertical infiltration of water into fractures or shafts. NP data from a site on the Mitchell Plain of southern Indiana, USA, revealed that NP data can be decomposed into three components: topographic effect, residual NP and noise. At this site, NP was inversely proportional to elevation, but the correlation varied with time. The topographic correction factor varied from –2.5 to –1.2 mV/m (NP change per unit elevation increase), with an average linear correlation coefficient (R) of 0.95. Because the site slopes toward an adjacent creek that is the local groundwater discharge zone, one possible explanation for this effect is a streaming-potential mechanism generated by groundwater movement toward the creek. The residual NP data revealed three negative anomalies at the survey area. Two of them coincide with sinkholes. A part of the third anomaly is coincident with a small valley, and concentrated infiltration does occur at this elevation in other valleys at the site, as evidenced by the existence of sinkholes. However, the dispersed, low-magnitude nature of the third anomaly does not prove the existence of concentrated groundwater recharge activity.
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  • 7
    ISSN: 0992-7689
    Keywords: Magnetospheric physics (auroral phenomena; storms and substorms)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Early auroral observations recorded in various oriental histories are examined in order to search for examples of strictly simultaneous and indisputably independent observations of the aurora borealis from spatially separated sites in East Asia. In the period up to AD 1700, only five examples have been found of two or more oriental auroral observations from separate sites on the same night. These occurred during the nights of AD 1101 January 31, AD 1138 October 6, AD 1363 July 30, AD 1582 March 8 and AD 1653 March 2. The independent historical evidence describing observations of mid-latitude auroral displays at more than one site in East Asia on the same night provides virtually incontrovertible proof that auroral displays actually occurred on these five special occasions. This conclusion is corroborated by the good level of agreement between the detailed auroral descriptions recorded in the different oriental histories, which furnish essentially compatible information on both the colour (or colours) of each auroral display and its approximate position in the sky. In addition, the occurrence of auroral displays in Europe within two days of auroral displays in East Asia, on two (possibly three) out of these five special occasions, suggests that a substantial number of the mid-latitude auroral displays recorded in the oriental histories are associated with intense geomagnetic storms.
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  • 8
    ISSN: 1432-0894
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract  The total ozone column is well correlated with tropospheric fields such as the heights of the upper tropospheric geopotential surfaces and thus it can provide useful information on temporal variability in the troposphere. The global availability of long period satellite measurements of the total ozone column, taken by the TOMS instruments since 1978, provides a valuable and independent data set for use in studies of seasonal and interannual climate variability. In this study, the global low-frequency seasonal teleconnections in the observed TOMS data from 1979–91 have been investigated using seasonal teleconnectivity maps and empirical orthogonal function analysis. They have also been compared with the results from a simulation made with the atmospheric GCM at Météo-France, having prescribed observed sea surface temperatures for the same period. In the observed total ozone, strong ENSO-related wave number one longitudinal dipole patterns are seen in both the tropics and in the Southern Hemisphere extratropics. The model shows much weaker variability in total ozone yet appears to be able to capture similar teleconnection patterns in the tropics related to ENSO. In the SH extratropics, the model total ozone shows a strong wave number 3 response rather than the wave number one dipole seen in the observations. A wave number 3 response is also evident in the 200 hPa geopotential height simulated by the model and in the NCEP analysis, and is consistent with the response in a linearised barotropic model forced in the Indonesian region. The different responses in the modelled and observed total ozone, suggest that tropopause effect is not the major factor in the SH extratropics, and it is likely that horizontal ozone transport also plays a role in this region. Despite a generally poor simulation of the zonal mean total ozone, the model was able to capture the anomalous strengthening of the SH stationary waves during austral spring of 1988, related to an intense stratosphere sudden warming.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 10 (1997), S. 236-240 
    ISSN: 1432-2145
    Keywords: Key words Cucurbita texana ; Pollen ; Pollen performance ; Pollen competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We examined the effects of pollen competition (pollen load size) on sporophytic vigor and gametophytic performance in Cucurbita texana, a wild gourd, while controlling for alternative interpretations of the data. Under field conditions we compared the vigor of progeny produced from large and small pollen loads and examined the in vitro performance of the pollen produced by the progeny. We found that the progeny from large pollen loads germinated faster and had a greater reproductive output (male flowers and fruits) than progeny produced from small pollen loads. In addition, we found that the pollen produced on plants derived from large pollen loads grew faster in vitro than the pollen produced on plants derived from small pollen loads. These findings indicate that pollen competition affects the performance of the resulting sporophytic generation and the microgametophytes they produce.
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  • 10
    ISSN: 1432-2145
    Keywords: Key words Cucurbita ; Pollen tube growth ; Pollen performance ; Hybridization ; Variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study examines sporophytic and gametophytic vigor in wild and cultivated varieties of Cucurbita pepo L. and their hybrids in order to determine whether hybrid vigor extends to the microgametophyte generation. It also examines the variation in sporophytic and gametophytic vigor to discern the non-genetic influences of pollen provisioning by the sporophyte on pollen performance from the genetic influences of the microgametophyte’s own genotype on pollen performance. A cultivated and a wild C. pepo and their F1 and the F2 generations were grown under field conditions and flower and fruit production were monitored over one summer. We found that the four types of plants differed significantly in the number of male and female flowers and the number of fruits they produced. The F1 plants produced significantly more male flowers and marginally more female flowers and fruits than the parental lines. To estimate gametophytic vigor pollen was germinated in vitro and pollen tube length measured after 30 min. We found that pollen tubes from the F1 plants had significantly greater growth than tubes from the parental lines or the F2 generation, indicating that hybrid vigor extends to the microgametophytic generation. By partitioning the variance of pollen tube growth into ’within’ and ’among’ plant components of variation, we were able to show that the genotype of the microgametophyte influences pollen performance in vitro, but that expression of hybrid vigor in the microgametophyte is likely to be due to an environmental effect related to provisioning of the pollen grains during development.
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