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  • 1
    Electronic Resource
    Electronic Resource
    Vibrio cholerae O139 produces a protease which is indistinguishable from the haemagglutinin/protease of Vibrio cholerae O1 and non-O1 (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 11 (1995), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Haemaglutinin/protease (HA/P) is one of the virulence factors of Vibrio cholerae O1 and pathogenic strains of V. cholerae non-O1. In this study, we examined protease activity of a new serogroup of Vibrio cholerae recently designated as O139 synonym Bengal. The protease activity was produced by all eight isolates of V. cholerae O139 from Bangladeshi patients. Purification and partial characterization of the protease from V. cholerae O139 demonstrated the purified protease (O139-P) was indistinguishable from that previously reported for HA/P of V. cholerae non-O1 (NAG-HA/P) and V. cholerae O1 (Vc-HA/P). These results prove that V. cholerae O139 produces a protease belonging to solHA/P, and suggest that the protease is another virulence factor found in newly emerged V. cholerae O139, as in V. cholerae O1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1995.tb00093.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ca2+independent cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin (TDH) on Intestine 407, a cell line derived from human embryonic intestine (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 134 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The effects of Vibrio parahaemolyticus thermostable direct hemolysin on Intestine 407, a cell line derived from the intestine of human embryo, were investigated. The hemolysin was shown to be cytotoxic to Intestine 407. This cytotoxicity is accompanied by the damage of plasma membrane and lysosomes, as well as cellular degeneration in the form of large transparent blebs. Although an increase in cytosolic free Ca2+ due to the influx of extracellular Ca2+ was observed in cells treated with thermostable direct hemolysin, it was found to be irrelevant to any of the above effects. These results suggest that the effects of thermostable direct hemolysin observed in this study on Intestine 407 are not mediated by Ca2+-dependent pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07943.x
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of a novel hemolysis gene of Vibrio cholerae (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 128 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A hemolysis gene (hlx) which lyses sheep erythrocytes on blood agar plates when expressed in Escherichia coli was cloned from Vibrio cholerae. The cloned gene is predicted to encode a polypeptide of 92 amino acid residues with a deduced molecular mass of 10451. E. coli transformed with this gene lysed sheep, goose, horse and chicken erythrocytes but not those of guinea pig and human. The hlx gene was observed in classical- and El Tor-biotype V. cholerae O1, V. cholerae non-O1, and V. mimicus, but not in V. parahaemolyticus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07534.x
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  • 4
    Electronic Resource
    Electronic Resource
    Zone Plate Common Path Interferometer for Testing Aspherical Mirror with Large Aperture (1995)
    Springer
    Optical review 2 (1995), S. 55-58 
    Details
    ISSN: 1349-9432
    Keywords: aspherical mirror ; large aperture ; common path interferometer ; caustic waist ; computer generated hologram ; diffraction efficiency balance ; laser writer ; phase shifting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new CGH (computer generated hologram) common path interferometer, which can be used for testing an aspherical mirror with large aperture, is proposed. This interferometer does not have a circle of least confusion, and a spatial filter can be used effectively to stop extraneous lights so that interferograms with good contrast can be obtained. The phase shifting technique can be easily applied to the interferometer. The method of designing and making the CGH zone-plate is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s10043-995-0055-5
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  • 5
    Electronic Resource
    Electronic Resource
    Radius Measurement of a Concave Mirror by a Zone Plate Interferometer (1995)
    Springer
    Optical review 2 (1995), S. 59-61 
    Details
    ISSN: 1349-9432
    Keywords: interferometry ; differential ; Newton gauge ; zone plate interferometer ; radius measurement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new technique to measure the radius of a concave mirror is proposed, in which the radius is calculated from the difference of the two interferograms obtained before and after shifting the sample perpendicular to or along the optical axis. By comparison of these two shifting directions, it is shown that since the amount of shift can be measured accurately and the result is insensitive to the tilt during the shifting, better accuracy can be obtained by shifting the sample along the optical axis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s10043-995-0059-1
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  • 6
    Electronic Resource
    Electronic Resource
    Alkaloidal oviposition stimulants for a danaid butterfly,Ideopsis similis L., from a host plant,Tylophora tanakae (Asclepiadaceae) (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 753-756 
    Details
    ISSN: 1420-9071
    Keywords: Oviposition stimulants ; Ideopsis similis ; Danaidae ; Tylophora tanakae ; Asclepiadaceae ; (+)-isotylocrebrine ; (−)-7-demethyltylophorine ; alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Chemicals releasing oviposition by an Asclepiadaceae feeder,Ideopsis similis, were identified from a host plant,Tylophora tanakae. A strong positive response was evoked by a methanolic extract of the plant, which proved to contain multiple stimulants. A mixture of two phenanthroindolizidine alkaloids, (+)-isotylocrebrine and (−)-7-demethyltylophorine, isolated from organic fractions, elicited significant ovipositional responses from females.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01941275
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  • 7
    Electronic Resource
    Electronic Resource
    Simulator for Aid of Trial-and-Error Design of Nonlinear Image Processing Filters (1995)
    Springer
    Optical review 2 (1995), S. 163-166 
    Details
    ISSN: 1349-9432
    Keywords: nonlinear filters ; image processing ; filter design method ; learning filter optimization ; mathematical morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A simulator to aid in the design of nonlinear image processing filters is developed. This simulator assists the filter design by determining parameters manually by trial-and-error. The method is also useful for improving the results of the learning optimization methods. An example of the application of the technique to improve a resultant filter by a learning method of a mathematical morphological filter is shown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s10043-995-0163-2
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  • 8
    Electronic Resource
    Electronic Resource
    An age-related decrease in the concentration of insulin-like growth factor binding protein-5 in human cortical bone (1995)
    Springer
    Calcified tissue international 57 (1995), S. 206-212 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The skeletal contents of insulin-like growth factor-2 (IGF-II), insulin-like growth factor binding protein-5 (IGFBP-5), and insulin-like growth factor binding protein-3 (IGFBP-3) were determined in duplicate samples of human femoral cortical bone obtained from 64 subjects (44 males and 20 females) between the ages of 20 and 64 years. The results of these quantitative measurements revealed an age-related decrease in the femoral cortical content of IGFBP-5 (r=-0.272, P=0.031) in the total population. Although the femoral cortical content of IGF-II did not show a similar decrease with age, it could be correlated to the femoral cortical content of IGFBP-5 (r=0.442, P〈0.001). In constrast, the femoral cortical content of IGFBP-3 did not decrease with age and could not be correlated to the femoral cortical contents of either IGFBP-5 or IGF-II. Comparisons of these results with previous measurements of insulin-like growth factor-1 (IGF-I) and transforming growth factor-β (TGF-β), in extracts of the same bones, showed significant cross-correlations between the femoral cortical contents of each of these growth factors and the femoral cortical contents of IGFBP-5 (r=0.625 for IGF-I versus IGFBP-5, r=0.554 for TGF-β versus IGFBP-5, P〈0.001 for each) but not IGFBP-3. Together, these data indicate average net losses of 60% and 29% of the femoral cortical contents of IGF-I and IGFBP-5, respectively, and apparent net losses (i.e., nonsignificant decreases) of 21% and 25% of the femoral cortical contents of IGF-II and TGF-β, respectively, between the third and the sixth decades (i.e., decreases from young adult values of 75.1 pmol/g of bone for IGF-I, 124.7 pmol/g of bone for IGF-II, 0.71 pmol/g of bone for TGF-β, 115.6 pmol/g of bone for IGFBP-5, and 26.2 pmol/g of bone for IGFBP-3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310260
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  • 9
    Electronic Resource
    Electronic Resource
    A neolignoid feeding deterrent againstLuehdorfia puziloi larvae (lepidoptera: Papilionidae) fromHeterotropa aspera, a host plant of sibling species,L. japonica (1995)
    Springer
    Journal of chemical ecology 21 (1995), S. 1541-1548 
    Details
    ISSN: 1573-1561
    Keywords: Feeding deterrent ; Luehdorfia puziloi ; L. japonica ; Papilionidae ; Parnassiinae ; Zerynthiini ; neolignoid ; asatone ; Heterotropa aspera ; Asiasarum sieboldii ; Aristolochiaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A feeding deterrent against larvae of a papilionid butterfly,Luehdorfia puziloi (Parnassiinae), a specialist onAsiasarum plants (Aristolochiaceae), was isolated from another aristolochiaceous plant,Heterotropa aspera. Antifeedant activity was exhibited by then-hexane-soluble fraction that proved to contain at least two active components. One of the deterrents was identified as a neolignan compound, asatone. The concentration of asatone inH. aspera was estimated at approximately 225 ppm, and larval feeding ofL. puziloi was significantly deterred at concentrations over 90 ppm. By contrast, asatone was not detectable (〈 1 ppm, if any) in its host plant,Asiasarum sieboldii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02035151
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic analysis of essential oil variants inPerilla frutescens (1995)
    Springer
    Biochemical genetics 33 (1995), S. 341-348 
    Details
    ISSN: 1573-4927
    Keywords: Perilla frutescens ; Labiatae ; chemotypes ; genetic analysis ; essential oils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A new chemotype (C type) ofPerilla frutescens (Labiatae) that accumulatestrans-citral as a main component of the essential oil in the leaf was crossed with five other chemotypes containing perillaldehyde (PA), elsholtziaketone (EK), perillaketone (PK), perillene (PL), and phenylpropanoid (PP) as their respective major components for comparison of genetic differences. The analyses of F1 and F2 progenies showed thattrans-citral is accumulated when its metabolism is blocked in the simultaneous absence of a dominant geneN, which is involved in the conversion oftrans-citral into naginataketone viacis-citral, and two polymeric genesFr 1 andFr 2 , which are involved in the conversion oftrans-citral into perillene. On the basis of new data obtained from various intercrosses involving the C type as one of the parents, the genotypes of different chemotypes as well as the sites of action of several genes controlling reaction steps in the biosynthesis of monoterpenes inPerilla have been revised.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553813
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