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  • Polymer and Materials Science  (375)
  • Life Sciences (General)  (74)
  • General Chemistry  (51)
  • 1995-1999  (500)
  • 1980-1984
  • 1940-1944
  • 1995  (500)
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  • 1995-1999  (500)
  • 1980-1984
  • 1940-1944
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  • 1
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The effect of surface roughness on osteoblast proliferation, differentiation, and protein synthesis was examined. Human osteoblast-like cells (MG63) were cultured on titanium (Ti) disks that had been prepared by one of five different treatment regimens. All disks were pretreated with hydrofluoric acid-nitric acid and washed (PT). PT disks were also: washed, and then electropolished (EP); fine sandblasted, etched with HCl and H2SO4, and washed (FA); coarse sandblasted, etched with HCl and H2SO4, and washed (CA); or Ti plasma-sprayed (TPS). Standard tissue culture plastic was used as a control. Surface topography and profile were evaluated by brightfield and darkfield microscopy, cold field emission scanning electron microscopy, and laser confocal microscopy, while chemical composition was mapped using energy dispersion X-ray analysis and elemental distribution determined using Auger electron spectroscopy. The effect of surface roughness on the cells was evaluated by measuring cell number, [3H]thymidine incorporation into DNA, alkaline phosphatase specific activity, [3H]uridine incorporation into RNA, [3H]proline incorporation into collagenase digestible protein (CDP) and noncollagenase-digestible protein (NCP), and [35S]sulfate incorporation into proteoglycan.Based on surface analysis, the five different Ti surfaces were ranked in order of smoothest to roughest: EP, PT, FA, CA, and TPS. A TiO2 layer was found on all surfaces that ranged in thickness from 100 Å in the smoothest group to 300 Å in the roughest. When compared to confluent cultures of cells on plastic, the number of cells was reduced on the TPS surfaces and increased on the EP surfaces, while the number of cells on the other surfaces was equivalent to plastic. [3H]Thymidine incorporation was inversely related to surface roughness. Alkaline phosphatase specific activity in isolated cells was found to decrease with increasing surface roughness, except for those cells cultured on CA. In contrast, enzyme activity in the cell layer was only decreased in cultures grown on FA- and TPS-treated surfaces. A direct correlation between surface roughness and RNA and CDP production was found. Surface roughness had no apparent effect on NCP production. Proteoglycan synthesis by the cells was inhibited on all the surfaces studied, with the largest inhibition observed in the CA and EP groups. These results demonstrate that surface roughness alters osteoblast proliferation, differentiation, and matrix production in vitro. The results also suggest that implant surface roughness may play a role in determining phenotypic expression of cells in vivo.
    Additional Material: 10 Ill.
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  • 2
    Publication Date: 2019-07-13
    Description: Apoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4+ T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to gamma-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA fragmentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in gamma-irradiated RH9 cells.
    Keywords: Life Sciences (General)
    Type: Scanning microscopy (ISSN 0891-7035); 9; 3; 833-42
    Format: text
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  • 3
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 33 (1995), S. 1545-1557 
    ISSN: 0887-6266
    Keywords: NMR relaxation ; PMR polyimides ; thermal degradation ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: We have studied cross-linking and thermal degradation of high-performance first-and second-generation PMR-15 polyimides, both thermoset and thermoplastic versions, by performing nonspectroscopic NMR solid echo T*2 relaxation measurements at temperatures up to 430°C using probes built for this purpose. We employ signal averaging and automated decomposition of the relaxation decays into two Gaussian components, the slower of which gradually appears above 300°C. Tracking the molecular mobility spectrum in terms of the relative intensity of the components and their relaxation times as temperature is cycled, we detect essentially no irreversible effects below the glass transition, measure permanent mobility reductions attributable to completion of cure, and find that exposure to temperatures above 380°C on the order of 1 h is required for substantial thermal degradation to occur. These results are closely supported by thermal and mechanical measurements on parallel specimens. Second-generation PMR resins appear to have higher microscopic rigidity and reduced viscous fraction at high temperatures. ©1995 John Wiley & Sons, Inc.
    Additional Material: 10 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 56 (1995), S. 1673-1677 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A series of oxazoline-modified styrene-acrylonitrile (SAN) copolymers was prepared through the reaction of amino alcohols onto preformed SAN copolymers. Consequently, a variety of reactive copolymers were synthesized. The major focus of this study was to examine the influence of reaction conditions, i.e., the nature of the catalyst and amino alcohol structure, on reactivity. The ability to incorporate oxazoline groups onto preformed polymers is dependent on whether homogeneous reaction conditions are met. For example, the use of nonreactive solubilizing agents, i.e., cosolvent, is effective. However, optimum conditions are obtained when the catalyst is completely soluble in the reaction mixture containing the amino alcohol and SAN copolymer. With these restrictions, zinc stearate is quite effective. Our results show that controlled and reproducible levels of oxazoline can be incorporated (typically at a rate of 2 mol %/h) up to high levels. These results are general and therefore are applicable to a wide variety of nitrile-containing polymers. © 1995 John Wiley & Sons, Inc.
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  • 5
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Novel EPDM (ethylenepropylenediene monomer) and polybutadiene polyols can be synthesized by a two-step process of controlled hydroformylation and then reduction of the formyl groups to place a desired amount of pendant alcohol groups along the polymer chain. The degree of functionalization can be controlled by measuring gas uptake from a calibrated reservoir during hydroformylation. Hydroformylation can be performed in solution or under simulated melt-phase conditions using either HRh(CO)(PPh3)3 or Rh(acac) (CO)2 as catalysts. Reduction of the polyaldehyde by NaBH4 generates the polymeric alcohol without further reaction of the remaining double bonds. Polymer functionalization and further modifications were followed by H-NMR and FTIR. These unique hydrophobic polyols can be reacted further to produce other polymer systems. As an example, urethanes have been made with these polyols by reaction with diisocyanates. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 1151-1155 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polyurethanes were synthesized by the reaction of hydroxyterminated polybutadiene (HTPB) and diisocyanates such as toluene diisocyanate (TDI), diphenylmethane diisocyanate (MDI), hexamethylene diisocyanate (HMDI), and isophoron diisocyanate (IPDI). A number of fillers such as carbon black, kaolin, and antimony trioxide (Sb2O3) were used in these formulations. Mechanical and thermal stability of these polyurethanes were studied. Based on its properties, HTPB-MDI derived filled polyurethane was selected and evaluated as an inhibitor for a composite propellant. © 1995 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
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  • 7
    Publication Date: 2011-08-24
    Description: Elliptical cells (E-P) are present at the perilymphatic interface lumen (PIL) of the lagena. The E-P cells often separate from the tegmentum vasculosum (TV) and have touching processes that form a monolayer between the K+ rich perilymph and the Na+ rich endolymph, similar to the mammalian Reissner's membrane. We examined the TV of chicks (Gallus domesticus) and quantitated the expression of anti-S100 alphaalphabetabeta and S100 beta. There was a 30% increase of S100 beta saturation in the light cells facing the PIL when compared to other TV light cells. We show that: (1) the dimer anti- S100 alphaalphabetabeta and the monomer anti-S100 beta are expressed preferentially in the light cells and the E-P cells of TV; (2) expression of S100 beta is higher in light cells facing the PIL than in adjacent cells; (3) the expression of the dimer S100 alphaalphabetabeta and monomer S100 beta overlaps in most inner ear cell types, including the cells of the TV, most S100 alphaalphabetabeta positive cells express S 100 beta, but S100 beta positive cells do not always express S100 alphaalphabetabeta; and (4) the S100 beta expression in light cells, the abundant Na+-K+ ATPase on dark cells of the TV, and previously demonstrated co-localization of S100 beta/GABA in sensory cells suggest that S100 beta could have, in the inner ear, a dual neurotrophic-ionic modulating function.
    Keywords: Life Sciences (General)
    Type: Scanning microscopy (ISSN 0891-7035); Volume 9; 4; 1207-21; discussion 1221-2
    Format: text
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  • 8
    Publication Date: 2011-08-24
    Description: Vitamin B-6 metabolism in 10 volunteers during 21 d of total fasting was compared with results from 10 men consuming a diet low only in vitamin B-6 (1.76 mumol/d) and with men consuming a normal diet during bed rest. At the end of the fast mean plasma concentrations of vitamin B-6 metabolites and urinary excretion of 4-pyridoxic acid tended to be higher in the fasting subjects than in the low-vitamin B-6 group. The fasting subjects lost approximately 10% of their total vitamin B-6 pool and approximately 13% of their body weight. The low-vitamin B-6 group lost only approximately 4% of their vitamin B-6 pool. Compared with baseline, urinary excretion of pyridoxic acid was significantly increased during 17 wk of bed rest. There was no increase in pyridoxic acid excretion during a second 15-d bed rest study. These data suggest the possibility of complex interactions between diet and muscle metabolism that may influence indexes that are frequently used to assess vitamin B-6 status.
    Keywords: Life Sciences (General)
    Type: The American journal of clinical nutrition (ISSN 0002-9165); Volume 62; 5; 979-83
    Format: text
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  • 9
    Publication Date: 2019-07-13
    Description: A novel organ culture system has been developed to study the regulation of statoconia production in the gravity sensing organ in Aplysia californica. Statocysts were cultured in Leibovitz (LI5) medium supplemented with salts and Aplysia haemolymph for four days at 17 C. The viability of the system was evaluated by examining four parameters: statocyst morphology, the activity of the mechanosensory cilia in the statocyst, production of new statoconia during culture and change in statoconia volume after culture. There were no morphological differences in statocysts before and after culture when ciliary beating was maintained. There was a 29% increase in the number of statoconia after four days in culture. Mean statocyst, statolith and statoconia volumes were not affected by culture conditions. The presence of carbonic anhydrase in the statocysts was shown using immunohistochemistry. When statocysts were cultured in the presence of 4.0 x 10(exp -4) M acetazolamide to inhibit the enzyme activity, there was a decrease in statoconia production and statoconia volume, indicating a role for this enzyme in statoconia homeostasis, potentially, via pH regulation. These studies are the first to report a novel system for the culture of statocysts and show that carbonic anhydrase is involved in the regulation of statoconia volume and production.
    Keywords: Life Sciences (General)
    Type: NASA-CR-204876 , NAS 1.26:204876 , Journal of Comparative Physiology A. Sensory, Neural and Behavioral Physiology; 177; 415-425
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 225 (1995), S. 21-35 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Dielektrische Messungen an Silikon, EPDM und einem Blend aus gleichen Gewichtsteilen beider Polymerer wurden im Frequenzbereich 1-105 Hz zwischen 25 und 200°C vor und nach Alterung in Wasser, Wasserdampf und Luft durchgeführt. Für das unpolare und nahezu verlustfreie EPDM werden niedrige Werte der Dielektrizitätskonstante und des Verlustfaktors gemessen. Die für EPDM beobachtete thermische Depolarisation ist bei in Luft gealtertem EPDM aufgrund von Dipolorientierung etwas ausgeprägter. Silikon wird in zwei Stufen polarisiert. Veränderungen der dielektrischen Eigenschaften und des spezifischen Gleichstromwiderstands nach Alterung belegen, daß Ladungsträger bei niedriger Temperatur und polare Silanolgruppen bei hoher Temperatur zur Polarisation beitragen. Die Eigenschaften des Blends hängen vom Alte-rungsmedium ab. Hydrothermische Alterung bewirkt eine qualitative Verbesserung des Dielektrikums Silikon durch Entfernung von Ladungsträgern und der Unterdrückung der Silikonhydrolyse durch EPDM. Durch Alterung des Blends in Luft nimmt dessen Polarisation zu, bedingt durch die Bildung von Ladungsträgern und Dipolen sowie die zusätzliche Grenzchicht. Aus Aktivierungsenergien wird gefolgert, daß die bei niedriger Temperatur stattfindende Polarisation mit der Beweglichkeit der Ladungsträger und der Orientierung von Seitengruppen zusammenhängt. rungsmedium ab. Hydrothermische Alterung bewirkt eine qualitative Verbesserung des Dielektrikums Silikon durch Entfernung von Ladungsträgern und der Unterdrückung der Silikonhydrolyse durch EPDM. Durch Alterung des Blends in Luft nimmt dessen Polarisation zu, bedingt durch die Bildung von Ladungsträgern und Dipolen sowie die zusätzliche Grenzchicht. Aus Aktivierungsenergien wird gefolgert, daß die bei niedriger Temperatur stattfindende Polarisation mit der Beweglichkeit der Ladungsträger und der Orientierung von Seitengruppen zusammenhängt.
    Notes: Dielectric measurements in the frequency (1-105 Hz) and temperature (25-200°C) domains have been carried out for silicone, EPDM, and their 50/50 (by weight) blend before and after ageing in water, steam and air. Low values of permittivity and loss tangent for EPDM reflect its non-polar and loss-free nature. Thermal depolarisation is also observed with EPDM and the air-aged specimen shows little rising trend due to dipole orientation. Silicone is found to be polarised in two steps. Changes in dielectric properties and DC resistivity on hydrothermal weathering and air ageing confirms that charge carriers contribute in the low-temperature step and polar silanol groups contribute in the high-temperature region. The properties of the 50:50 blend are very much sensitive to the medium of ageing. Hydrothermal ageing improves the quality of the dielectric of silicone due to removal of charge carriers and the suppression of silicone hydrolysis by EPDM. Ageing of the blend in air increases the polarisation to a very high level owing to the charge carriers, the additional interface and the dipoles formed during ageing. Activation energies suggest that the low temperature polarisation step is associated with charge carrier mobility and orientation of pendent groups.
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