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  • Two-dimensional polyacrylamide gel electrophoresis  (3)
  • Genetics  (2)
  • soils  (2)
  • 1995-1999  (7)
  • 1985-1989
  • 1995  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Extractable chromium determination in soils by AAS (1995)
    Springer
    Microchimica acta 119 (1995), S. 251-258 
    Details
    ISSN: 1436-5073
    Keywords: extractable chromium ; soils ; EDTA extracts ; acetic acid extracts ; FAAS ; releasing agent oxine and ETAAS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The lixiviation of trace metals from soils using selective extractants gives valuable information, especially for agricultural purposes. The two reagents validated by a group of European researchers coordinated by the Measurements and Testing Program (MAT) of the Commission of the European Community, in single extraction procedures, are EDTA 0.05 moll−1 and CH3COOH 0.43 moll−1. This paper reports the effect of these extractants upon chromium determination in extracts by AAS. Matrix effects were studied in extracts of two soils of different origin: a terra rossa soil from a Mediterranean area and a sewage amended soil. For the FAAS technique, the matrix effects were studied for air-acetylene and nitrous oxide-acetylene flames and using Cr(VI) and Cr(III) as standard solutions. The interfering effects observed are circumvented by the use of 1% 8-hydroxyquinoline as suppressor agent. For the ETAAS technique, charring and atomization curves were obtained for extracts in each reagent and after setting up the graphite furnace program, matrix effects were studied. Different matrix modifiers (Mg(NO3)2, Pd and Triton X-100) were also tested. Once the analytical method had been established, single extraction procedures were applied in the two soils, determining the percentage of extractable chromium in relation to the total content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244004
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of candidate certified reference materials for the quality control of EDTA-and acetic acid-extractable trace metal determinations in sewage sludge-amended soil and terra rossa soil (1995)
    Springer
    Microchimica acta 120 (1995), S. 289-300 
    Details
    ISSN: 1436-5073
    Keywords: extraction ; soils ; trace elements ; candidate CRMs ; reference materials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The determination of extractable trace metal contents in soil using single extraction procedures is currently performed in many laboratories to assess the bioavailable metal fraction (and related phytotoxic effects) and the accessability to the environment (e.g. contamination of ground waters). Owing to the need for validation of the extraction schemes used and of the analytical techniques, the EC Measurements and Testing Programme (formerly BCR) has organized a project for improving the quality of determination of extractable trace metal contents in soil, the first step being an interlaboratory study to adopt common extraction procedures and the second being a certification campaign to certify two soils for their extractable trace element contents following these procedures. This paper gives a brief overview of the project organisation and describes the preparation, homogeneity and stability studies of two soil candidate reference materials (sewage sludge-amended and terra rossa soils).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244439
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  • 3
    Electronic Resource
    Electronic Resource
    Phenotyping of apolipoprotein E using immobilized pH gradient gels for one-dimensional and two-dimensional separations (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1184-1186 
    Details
    ISSN: 0173-0835
    Keywords: Apolipoprotein E ; Immobilized pH gradient ; Phenotyping ; Immunoblotting ; Two-dimensional polyacrylamide gel electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Apolipoprotein E (apo E) is a normal component of several classes of plasma lipoproteins. Apo E phenotypes are closely related to total cholesterol, low density lipoprotein (LDL)-cholesterol and apo B concentration. The apo E 2/2 phenotype is related to the type III hyperlipoproteinemia due to the defective binding of apo E-2 to the hepatic receptors. The apo E 4/4 phenotype has been reported to be present in most elderly people suffering from the Alzheimer disease, and is associated with increased risk of coronary heart disease and Creutzfeld-Jakob disease. Therefore, apo E phenotyping is essential. The method described here uses a precast immobilized pH gradient, avoids time-consuming separation of lipoproteins from plasma, needs no pretreatment with neuraminidase and involves highly sensitive enhanced chemiluminescence for visualization. Therefore it has many advantages over previously published methods.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601196
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of the URA5 gene from the yeast Yarrowia lipolytica (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 425-433 
    Details
    ISSN: 0749-503X
    Keywords: Yarrowia lipolytica ; orotate phosphoribosyl transferase ; nucleotide sequence ; transcription ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The URA5 gene of Yarrowia lipolytica encoding the orotate phosphoribosyl transferase (OPRTase, EC2.4.2.10) was isolated by target integration in a mutant strain originally named ura2.21. The nucleotide sequence of the gene predicts a protein with high similarities with the OPRTases from Saccharomyces cerevisiae, Podospora anserina and Escherichia coli and to a lesser extent with that of Dictyostelium discoideum. The transcription start point has been mapped by primer extension analysis and indicates the existence of a long leader sequence in the corresponding mRNA. Northern-blot hybridization revealed the URA5 transcript to be approximately 0·94 kb. Deletion of the URA5 gene in Y. lipolytica produced a leaky phenotype similar to the one described for the ura5 mutation in S. cerevisiae. The URA5 gene of Y. lipolytica was able to complement functionally the ura5 mutation of S. cerevisiae. The sequence presented here has been submitted to the EMBL data library under Accession Number Z22571.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110505
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  • 5
    Electronic Resource
    Electronic Resource
    The complete sequence of a 9037 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 587-591 
    Details
    ISSN: 0749-503X
    Keywords: yeast genome ; chromosome VII ; histidine permease ; glyceraldehyde-3-phosphate dehydrogenase ; pyruvate dehydrogenase ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the sequence of a 9037 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII. Analysis of the sequence revealed four complete open reading frames (ORFs), namely G7572, G7576, G7579 and G7584. The first three corresponded, respectively, to the previously cloned genes: HIP1, coding for a high-affinity histidine-specific permease, TDH1, one of the known genes coding for glyceraldehyde-3-phosphate dehydrogenase and ODPX, which encodes a precursor of protein X, a component of the pyruvate dehydrogenase complex. The ORF G7584 showed 35·8% identity with a hypothetical protein of Caenorhabditis elegans chromosome 3. The reported sequence has been deposited in the EMBL data library under Accession Number X82408.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110609
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  • 6
    Electronic Resource
    Electronic Resource
    Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1152-1159 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Human blood platelets ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional (2-D) maps of cytosol and enriched-membrane platelet proteins has allowed the identification of more than 25 spots by three different methods: matching of the platelet gels with other 2-D reference maps, immunoblotting with chemiluminescence detection, and N-terminal sequencing. Different G protein (guanosine triphosphate-binding protein) subunits, cytoskeletal proteins, and proteins common to the human liver, red blood cells and plasma were identified. The two platelet protein maps presented here contribute to the project of identification of human cell and body fluid proteins. They may serve as working tools since platelets are popular models for the study of central nervous system neurotransmitter systems and stimulus-response coupling mechanisms.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601191
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  • 7
    Electronic Resource
    Electronic Resource
    Spermatocytes and round spermatids of rat testis: Protein patterns (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1225-1230 
    Details
    ISSN: 0173-0835
    Keywords: Testis ; Spermatogenesis ; Two-dimensional polyacrylamide gel electrophoresis ; Protein map ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Spermatogenesis is a process in the testis that involves meiotic cell division and spermiogenesis. The mechanisms of regulation and its associated proteins are mostly unknown. This publication shows the two-dimensional (2-D) gel electrophoresis protein map obtained from rat testis using nonlinear 3.5-10 immobilized pH gradients for the first-dimensional separation. Eighteen proteins were successfully identified in the SWISS-PROT protein database using amino acid analysis of proteins recovered from polyvinylidene difluoride (PVDF) membranes and verified for one of them by comparison with Anderson's rat liver reference map. Fourteen new polypeptides were identified and four were previously known. Two of these new proteins were closely related to the spermatogenetic process. T-complex protein 1 is expressed in large amounts in germ cells. Androgen-dependent sperm-coating glycoprotein is secreted by epididymal cells. In order to detect changes in protein expression during meiosis and spermiogenesis, spermatocytes and round spermatid cell populations were purified by centrifugal elutriation and compared. In this way several proteins not found in the spermatocyte 2-D images could be highlighted. The sperm-coating glycoprotein was thus shown to be present in large amounts in round spermatids.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601203
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