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  • Biochemistry and Biotechnology  (8)
  • Genetics  (2)
  • soils  (2)
  • 1995-1999  (12)
  • 1985-1989
  • 1995  (12)
  • 1
    Electronic Resource
    Electronic Resource
    Extractable chromium determination in soils by AAS (1995)
    Springer
    Microchimica acta 119 (1995), S. 251-258 
    Details
    ISSN: 1436-5073
    Keywords: extractable chromium ; soils ; EDTA extracts ; acetic acid extracts ; FAAS ; releasing agent oxine and ETAAS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The lixiviation of trace metals from soils using selective extractants gives valuable information, especially for agricultural purposes. The two reagents validated by a group of European researchers coordinated by the Measurements and Testing Program (MAT) of the Commission of the European Community, in single extraction procedures, are EDTA 0.05 moll−1 and CH3COOH 0.43 moll−1. This paper reports the effect of these extractants upon chromium determination in extracts by AAS. Matrix effects were studied in extracts of two soils of different origin: a terra rossa soil from a Mediterranean area and a sewage amended soil. For the FAAS technique, the matrix effects were studied for air-acetylene and nitrous oxide-acetylene flames and using Cr(VI) and Cr(III) as standard solutions. The interfering effects observed are circumvented by the use of 1% 8-hydroxyquinoline as suppressor agent. For the ETAAS technique, charring and atomization curves were obtained for extracts in each reagent and after setting up the graphite furnace program, matrix effects were studied. Different matrix modifiers (Mg(NO3)2, Pd and Triton X-100) were also tested. Once the analytical method had been established, single extraction procedures were applied in the two soils, determining the percentage of extractable chromium in relation to the total content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244004
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of candidate certified reference materials for the quality control of EDTA-and acetic acid-extractable trace metal determinations in sewage sludge-amended soil and terra rossa soil (1995)
    Springer
    Microchimica acta 120 (1995), S. 289-300 
    Details
    ISSN: 1436-5073
    Keywords: extraction ; soils ; trace elements ; candidate CRMs ; reference materials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The determination of extractable trace metal contents in soil using single extraction procedures is currently performed in many laboratories to assess the bioavailable metal fraction (and related phytotoxic effects) and the accessability to the environment (e.g. contamination of ground waters). Owing to the need for validation of the extraction schemes used and of the analytical techniques, the EC Measurements and Testing Programme (formerly BCR) has organized a project for improving the quality of determination of extractable trace metal contents in soil, the first step being an interlaboratory study to adopt common extraction procedures and the second being a certification campaign to certify two soils for their extractable trace element contents following these procedures. This paper gives a brief overview of the project organisation and describes the preparation, homogeneity and stability studies of two soil candidate reference materials (sewage sludge-amended and terra rossa soils).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244439
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  • 3
    Electronic Resource
    Electronic Resource
    Enhancement and inhibition of luminol chemiluminescence by phenolic acids (1995)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 10 (1995), S. 175-184 
    Details
    ISSN: 0884-3996
    Keywords: Luminol ; enhanced chemiluminescence ; phenolic acid ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We explored the behaviour of a series of phenolic acids used as enhancers or inhibitors of luminol chemiluminescence by three different methods to determine if behaviour was associated with phenolic acid structure and redox character. All the phenolic acids inhibited chemiluminescence when hexacyanoferrate(III) was reacted with the phenolic acids before adding luminol. The redox character of these compounds was clearly related to structure. When hexacyanoferrate(III)-luminol-O2 chemiluminescence was initiated by phenolic acid-luminol mixtures some phenolic acids behaved as enhancers of chemiluminescence, and others as inhibitors. We propose a mechanism to explain these findings. We found direct relationships between the redox character of the phenolic acids and the enhancement or inhibition of the chemiluminescence of the luminol-H2O2-peroxidase system and we propose mechanism to explain these phenomena.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170100306
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the conformational domains of bradykinin by computational methods (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Peptide Science 1 (1995), S. 227-235 
    Details
    ISSN: 1075-2617
    Keywords: Bradykinin ; conformational domains ; conformational search ; molecular mechanics ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The AMBER 4.0 force field was used to perform a characterization of the conformational profile of the nonapeptide bradykinin. A thorough conformational search was carried out using molecular dynamics as sampling technique, by computing cycles of high (900 K) and low (300 K) temperature trajectories. A total of 2400 minima were generated and subsequently clustered using the root-mean-square of the backbone dihedral angles as criterium. After the use of a tolerance value of 20deg;, the conformations were clustered in 233 unique conformations with energies up to 40 kcal/mol above the lowest minimum. The analysis of the low-energy conformations indicate that the peptide exhibits a high tendency to adopt a β-turn at the C-terminus and a propensity to adopt a bent structure at the N-terminus. These results are in agreement with the experimental evidence reported in the literature and provide detailed information necessary to understand the conformational preferences of the peptide.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/psc.310010403
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  • 5
    Electronic Resource
    Electronic Resource
    Assay of cholinesterase using a proenhancer of the luminol-H2O2-horseradish peroxidase reaction (1995)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 10 (1995), S. 285-289 
    Details
    ISSN: 0884-3996
    Keywords: Cholinesterase ; luminol ; pro-enhancer ; chemiluminescence ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: 2-Naphthyl acetate acts as a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction. Cholinesterase hydrolyses the bound acetyl group and produces 2-naphthol, and this compound is an enhancer of the chemiluminescent reaction. We studied the kinetics of chemiluminescent emission and the influence of 2-naphthyl acetate and cholinesterase enzyme concentration. The cholinesterase concentration versus chemiluminescence intensity maximum was linear for cholinesterase between 0 and 181 μU/mL, with a detection limit of 8 μU/mL and a relative standard deviation of 9.5% (n = 3), for a sample containing 90.67 μU/mL of cholinesterase.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170100505
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  • 6
    Electronic Resource
    Electronic Resource
    Inside SWISS-2DPAGE database (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1131-1151 
    Details
    ISSN: 0173-0835
    Keywords: SWISS-2DPAGE ; Two-dimensional gel electrophoresis ; Protein database ; Protein mapping ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Several two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) databases have been established and updated for more than 15 years. Only recently have developments of computer networks and high-speed transfer protocols provided the required tools for sharing comprehensive and hypermedia 2-D PAGE databases. This publication describes the SWISS-2DPAGE database structure. Proteins present in samples of human tissue, cells, cell lines and body fluids are assembled and described in an accessible uniform format. SWISS-2DPAGE can be freely accessed through the World-Wide Web (WWW) network on the ExPASy molecular biology server.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601190
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  • 7
    Electronic Resource
    Electronic Resource
    Phenotyping of apolipoprotein E using immobilized pH gradient gels for one-dimensional and two-dimensional separations (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1184-1186 
    Details
    ISSN: 0173-0835
    Keywords: Apolipoprotein E ; Immobilized pH gradient ; Phenotyping ; Immunoblotting ; Two-dimensional polyacrylamide gel electrophoresis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Apolipoprotein E (apo E) is a normal component of several classes of plasma lipoproteins. Apo E phenotypes are closely related to total cholesterol, low density lipoprotein (LDL)-cholesterol and apo B concentration. The apo E 2/2 phenotype is related to the type III hyperlipoproteinemia due to the defective binding of apo E-2 to the hepatic receptors. The apo E 4/4 phenotype has been reported to be present in most elderly people suffering from the Alzheimer disease, and is associated with increased risk of coronary heart disease and Creutzfeld-Jakob disease. Therefore, apo E phenotyping is essential. The method described here uses a precast immobilized pH gradient, avoids time-consuming separation of lipoproteins from plasma, needs no pretreatment with neuraminidase and involves highly sensitive enhanced chemiluminescence for visualization. Therefore it has many advantages over previously published methods.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601196
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  • 8
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of the URA5 gene from the yeast Yarrowia lipolytica (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 425-433 
    Details
    ISSN: 0749-503X
    Keywords: Yarrowia lipolytica ; orotate phosphoribosyl transferase ; nucleotide sequence ; transcription ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The URA5 gene of Yarrowia lipolytica encoding the orotate phosphoribosyl transferase (OPRTase, EC2.4.2.10) was isolated by target integration in a mutant strain originally named ura2.21. The nucleotide sequence of the gene predicts a protein with high similarities with the OPRTases from Saccharomyces cerevisiae, Podospora anserina and Escherichia coli and to a lesser extent with that of Dictyostelium discoideum. The transcription start point has been mapped by primer extension analysis and indicates the existence of a long leader sequence in the corresponding mRNA. Northern-blot hybridization revealed the URA5 transcript to be approximately 0·94 kb. Deletion of the URA5 gene in Y. lipolytica produced a leaky phenotype similar to the one described for the ura5 mutation in S. cerevisiae. The URA5 gene of Y. lipolytica was able to complement functionally the ura5 mutation of S. cerevisiae. The sequence presented here has been submitted to the EMBL data library under Accession Number Z22571.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110505
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  • 9
    Electronic Resource
    Electronic Resource
    The complete sequence of a 9037 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 587-591 
    Details
    ISSN: 0749-503X
    Keywords: yeast genome ; chromosome VII ; histidine permease ; glyceraldehyde-3-phosphate dehydrogenase ; pyruvate dehydrogenase ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the sequence of a 9037 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII. Analysis of the sequence revealed four complete open reading frames (ORFs), namely G7572, G7576, G7579 and G7584. The first three corresponded, respectively, to the previously cloned genes: HIP1, coding for a high-affinity histidine-specific permease, TDH1, one of the known genes coding for glyceraldehyde-3-phosphate dehydrogenase and ODPX, which encodes a precursor of protein X, a component of the pyruvate dehydrogenase complex. The ORF G7584 showed 35·8% identity with a hypothetical protein of Caenorhabditis elegans chromosome 3. The reported sequence has been deposited in the EMBL data library under Accession Number X82408.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110609
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  • 10
    Electronic Resource
    Electronic Resource
    Retinoic acid enhances connexin43 expression at the post-transcriptional level in rat liver epithelial cells (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 13 (1995), S. 69-77 
    Details
    ISSN: 0263-6484
    Keywords: Gap junctions ; retinoic acid ; liver cells ; connexin43 ; up-regulation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The mechanism by which all-trans retinoic acid (RA) stimulates gap junctional intercellular communication (GJIC) in the rat liver epithelial cell line. IAR203, was investigated. When RA, at 0·1 μM for 24-48 h, enhanced the dye transfer in IAR203 cells (× 1·4), it increased the amount of connexin43 (Cx43) in the cell-cell contact regions of the plasma membrane, as evidenced by analysis by Western blot and by immunofluorescence. It had no effect on the level of Cx43 mRNA. Freeze-fracture analysis of the size of gap junctions revealed an increase of the proportion of small gap junctions in RA-treated cells. We conclude that, in IAR203 cells, RA stimulates GJIC by acting at the post-tran-scriptional level of Cx43 regulation. The possibility that RA acts indirectly on the regulation of Cx43 expression, and increases the helf-life of Cx43 by inducing adhesion molecules is discussed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290130112
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