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  • Springer  (42)
  • American Geophysical Union  (7)
  • International Union of Crystallography (IUCr)  (3)
  • Geological Society of America (GSA)
  • Periodicals Archive Online (PAO)
  • 2000-2004
  • 1995-1999  (53)
  • 1950-1954
  • 1998  (23)
  • 1995  (30)
  • 1
    ISSN: 1573-2657
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The main objective of this study was to analyse glycogen in single muscle fibres, using a recently developed microfluorometric method which detects subpicomol amounts of NADPH, glucose and glycogen (as glucosyl units) (detection limit 0.16–0.17pmol in a 25nl sample) without fluorochrome amplification. The fibres were freshly dissected from the twitch region of the iliofibularis muscle of the cane toad (Bufo marinus), and were mechanically skinned under paraffin oil to gain access to the intracellular compartments. The results show that: (1) glycogen concentrations in toad skeletal muscle fibres range between 25.8 and 369mmol glucosyl units/litre fibre volume; (2) there is a large variation in glycogen content between individual fibres from the iliofibularis muscle of one animal; (3) there are seasonal differences in the glycogen content of toad single muscle fibres; (4) the total amount of glycogen in single muscle fibres of the toad does not decrease significantly when storing the tissue, under paraffin oil, at 20–25°C for up to 6h or at 4°C for up to 24h; and (5) 15–26% of fibre glycogen can be washed in an aqueous solution at pH 5–7, within 5min, while 74–85% of fibre glycogen remains associated with the washed skinned fibre, even after 40min exposure of the skinned fibre preparation to the aqueous environment. The retention of most glycogen in the fibre preparation after mechanical removal of the plasma membrane and extensive washing indicates that in toad skeletal muscle fibres the largest proportion of glycogen is tightly bound to intracellular structures. The results also show that the skinned muscle fibre preparation is well suited for microfluorometric glycogen determination, since low molecular weight non-glycogen contributors to the fluorescence signal can be removed from the myoplasmic space prior to the glycogen hydrolysis step.
    Type of Medium: Electronic Resource
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  • 2
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    Dordrecht : Periodicals Archive Online (PAO)
    Journal of Business Ethics. 14:2 (1995:Feb.) 145 
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  • 3
    Electronic Resource
    Electronic Resource
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 2 (1995), S. 163-173 
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: Perfect crystals in the asymmetric Bragg geometry are evaluated as optical elements for manipulating coherent X-ray beams. Such optics can be used to modify the transverse coherence length of a synchrotron X-ray beam, with the intention of increasing the usable coherent flux. The wavelength range, angular divergence and flux of X-rays passing through a pinhole aperture are examined in detail, as functions of source and pinhole size, crystal-to-pinhole separation and the asymmetry factor. In developing this analysis, the behavior of asymmetrically cut crystals is explained in reciprocal space, with reference to the crystal truncation rod associated with the reflection. The results show that, for synchrotron beams that are collimated to a small fraction of the incident Darwin width, the wavelength range accepted by the crystal is typically dispersed into an angular spread in the exit beam. This chromatic aberration greatly reduces the transverse coherence length in a manner that does not conserve the coherent flux. The calculations are in agreement with measurements of the divergence and flux through a micrometer-sized pinhole using a synchrotron wiggler X-ray source.
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  • 4
    Electronic Resource
    Electronic Resource
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 5 (1998), S. 37-47 
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: Following the completion of the collaborative project between CLRC Daresbury Laboratory and EG&G ORTEC to develop the world's first 30-element HPGe detector for fluorescence XAFS, it has now been tested and commissioned at the SRS. The system was commissioned with the XSPRESS digital pulse-processing electronics and this has demonstrated processed count rates in excess of 10 MHz. Initial data have been recorded and are presented.
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  • 6
    ISSN: 1432-1890
    Keywords: Key words Mycorrhizae ; Arctic-alpine ; Dark septate fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Roots of 40 taxa of higher plants (Pteridophyta, Spermatophyta) from two alpine study sites in Denali National Park and Preserve in central Alaska were examined for their mycorrhizal colonization. We observed ectomycorrhizae on six species: Betula nana, Salix reticulata, Salix polaris, Salix arctica, Polygonum viviparum, and Dryas octopetala. Seven taxa, Cassiope tetragona, Empetrum nigrum, Ledum palustre subsp. decumbens, Ledum palustre subsp. groenlandicum, Loiseleuria procumbens, Vaccinium uliginosum and Vaccinium vitis–idaea (all Ericales), had ericoid mycorrhizae. One species, Arctostaphylos alpina, formed a typical arbutoid mycorrhiza. Two species (Sibbaldia procumbens and Aconitum delphinifolium) showed well-developed VA mycorrhizae, whereas three species of plants (Lycopodium clavatum, Silene acaulis and Oxytropis scammaniana) had vesicles, but no arbuscules. The roots of 11 other plants (Lycopodium clavatum, Lycopodium selago, Silene acaulis, Gentiana algida, Lupinus arcticus, Oxytropis scammaniana, Pedicularis langsdorffii, Pedicularis capitata, Pedicularis verticillata, Artemisia sp. and Carex bigelowii) had a variety of intracellular colonizations which are referred to as dark septate fungi. No mycorrhizae were found on 12 other plants: Equisetum arvense, Equisetum variegatum, Lycopodium alpinum, Polygonum bistorta, Saxifraga hieracifolia, Saxifraga hirculus, Astragalus alpinus, Pedicularis kanei, Petasites frigidus, Carex podocarpa, Carex microchaeta and Poa arctica. A possible ecological role of dark septate fungi is discussed.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 29 (1995), S. 344-350 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The acute toxicity (96-h LC50) of aqueous stable iodine species (I−, IO 3 − , I2) to rainbow trout and Daphnia magna were measured at three individual concentrations of hardness, total organic carbon, and chloride. Rainbow trout were most sensitive to I2 (LC50⩾0.53 mg/L), and much less sensitive to IO 3 − (LC50⩾220 mg/L) or I− (LC50⩾860 mg/L). Daphnia magna were equally sensitive to I2 (LC50⩾0.16 mg/L) and I− (LC50⩾0.17 mg/L), but were less sensitive to IO 3 − (LC50⩾10.3 mg/L). The external and internal radiological dose imparted by equivalent molar quantities of radioactive 125I, 129I, and 131I were calculated for both the Daphnia and trout using the LC50 values obtained from a standard water treatment. As expected, the dose from 125I and 131I would exceed the expected lethal dose rate long before a chemically toxic level is reached. In contrast, a molar concentration of 129I likely to cause death by chemical toxicity would impart a radiological dose less than that expected to be lethal. Thus, for short-lived aquatic organisms, risks due to chemical toxicity of 129I may exceed risks due to its radioactive emissions.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 28 (1995), S. 443-450 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The California State Mussel Watch program is a long-term monitoring program conducted by the State Water Resources Control Board and the Department of Fish and Game. In a fifteen year time span, from 1977–1992, mussels were collected at 378 stations. From these stations, 47 were chosen to conduct statistical analysis based on the criteria that they had been sampled at least 6 times for total DDTs, total polychlorinated biphenyls (PCBs), and total chlordanes. Declines of total DDTs and chlordanes were noted at approximately half of the stations. Declines of total PCBs were noted at approximately one-quarter of the stations. Declines of PCBs but not DDTs in mussels near Los Angeles County's sewer discharge corresponded to declines of these contaminants in treated effluent.
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  • 9
    ISSN: 1432-0630
    Keywords: PACS: 68.55; 72.20; 81.10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Climate dynamics 11 (1995), S. 115-128 
    ISSN: 1432-0894
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The Southern Oscillation (SO) is examined in three 10 year AMIP-type integrations of a 30-level GCM having prescribed monthly mean observed sea surface temperatures for the period January 1979 to December 1988. Three horizontal spectral resolutions of T21, T42 and T79 are investigated and the results are compared with the low-frequency variability, having periods longer than 8 months, in the observed Darwin and Tahiti sea level pressures (SLP) and in the T106 ECMWF analyses from May 1985 to April 1991. Both the ECMWF analyses and the GCM results give unrealistic SLP variability at Tahiti resulting in low Darwin-Tahiti SLP correlations and low S/N ratios for the Tahiti-Darwin SO index. The ECMWF analyses are in particularly poor agreement with the observations during 1987 with anomalously high SLP at Tahiti. Examination of the ECMWF assimilated SSTs, reveals that this may be related to the assimilated SSTs being too cold in the central and eastern equatorial Pacific during mid-1987. The GCM results show the familiar SLP dipole in the tropical Pacific albeit displaced eastwards compared to previous observational studies especially at T42 resolution, thus accounting for the problems at Tahiti which lies near strong gradients in the correlation pattern. Time-longitude diagrams of low-level convergence and correlation maps of upper-level streamfunction suggest that the model is reproducing the SO divergence anomalies although too weakly at T21 resolution and at different longitudinal locations at T42 and T79 resolutions. The time-mean low-level convergences in the GCM simulations give ITCZs and SPCZs in qualitative agreement with the observations with a tendency for increased convergence in the eastern Pacific ITCZ at higher resolution. Longitudinal shifts are not apparent in the time-mean convergence when comparing the GCM results at different resolutions.
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