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  • Blackwell Publishing Ltd  (14)
  • 1995-1999  (14)
  • 1990-1994
  • 1980-1984
  • 1997  (4)
  • 1995  (10)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 123 (1995), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: We have investigated the shear-wave polarization anisotropy in the north-eastern Japan arc by using waveforms from many local earthquakes at various depths. We used a cross-correlation method to detect the shear-wave splitting. For intermediate-depth and deep events, the observed fast shear-wave oscillation directions (FSODs) are oriented parallel to the dip direction of the slab at the western stations, while they are oriented perpendicular to the dip direction at the eastern stations. These observations indicate that significant anisotropy exists in the mantle wedge, and that its nature is quite different between the eastern and the western parts.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 120 (1995), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: A remarkable later phase has been detected in seismograms between P- and S-wave arrivals at epicentral distances of about 150 km or more for events occurring in the north-eastern Japan arc. The characteristics of this phase support an interpretation as an sP phase at small epicentral distances, an upgoing S wave from the focus which is then reflected and converted to a P wave at the Earth's surface, diving into the Earth again, and finally reaching the observation stations. The arrival-time difference between this phase and the first P wave is very sensitive to the focal depths of events, and is useful for accurate estimation of the focal depths of those events, in a similar way to using teleseismic depth phases of pP and sP. The utilization of this phase has the advantage of yielding estimates of focal depths for relatively small magnitude events that do not have enough energy to generate teleseismic waves. By using the observed sP phases at small epicentral distances, shallow events beneath the Pacific Ocean in the north-eastern Japan arc, whose focal depths are poorly constrained by the land seismograph network of Tohoku University, have been relocated. The estimated hypocentre distribution, together with the focal mechanism solutions of some of the events, shows that the Pacific plate beneath this region subducts at an extremely low dip angle of less than 10d̀ for the first descent to about 20 km depth and then gradually becomes a steeper dip angle of about 30d̀ at depths deeper than 30 km.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 60 (1995), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Juice and seeds from 16 cultivars of mature pummelo fruits were analyzed for limonoid content. Pummelo juice contained an average of 18 ppm limonin and 29 ppm total limonoid glucosides. Compared to other juices, ptmnnelo contained very high concentrations of limonin and very low concentrations of limonoid glucosides. Limonin, nomilin, obacunone and trace amounts of deacetyhromilin were found in pummelo seeds. The 17–β-D-glucopyranoside derivatives (glucosides) of nomilin, nomilinic acid and obacunone were also present. Total limonoid aglycone concentration in the seeds ranged from 773 ppm to 9,900 ppm and total limonoid glucosides ranged from 130 ppm to 1,912 ppm.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 101 (1997), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Proline-overproducing carrot cell lines were isolated by selection in medium containing hydroxyproline, a toxic analogue of proline. During growth of the cells in culture, length of lag phase, doubling time, and maximum fresh weight were the same for the hydroxyproline-resistant cell line (HP) and the wild-type cell line (JW). Proline content and resistance to hydroxyproline in the HP and JW lines were not strictly correlated indicating that another reason besides the constitutive level of proline is involved in hydroxyproline resistance. Tolerance to polyethylene glycol-induced desiccation stress was not different between the two lines except perhaps at the early stages of culture growth when the proline levels of the two cell lines were nearly the same. The complexity of the relationship between proline accumulation and osmotolerance is discussed and strategies to achieve constitutive high levels of proline accumulation in plants are proposed.
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  • 5
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Heat-stable mycelial extracts of the nonpathogenic fungus Trichoderma longibrachiatum induced resistance in tobacco seedlings (Nicotiana tabacum L. cv. Wisconsin 38) to the pathogen Phytophthora parasitica var. nicotianae (race 0), which did not involve a hypersensitive response. Resistance could not be induced with mycelial extract prepared in the same manner from P. parasitica. The nonpathogenic mycelial extract induced expression of PR-1b and osmotin (PR-5) genes to a higher level than did mycelial extract from the pathogenic fungus. The tissue-specific pattern of PR gene induction by the nonpathogenic mycelial extract was different from that of the pathogenic mycelial extract and was consistent with the ability of the former to cause disease resistance. The expression patterns of these two PR genes and the accumulations of their encoded proteins also were affected by salicylic acid (SA), methyl jasmonate (MeJA), ethylene (E) and combinations of these plant signal messengers. However, only combined SA and MeJA treatment mimicked the pattern of PR gene mRNA and protein accumulation induced by the nonpathogenic mycelial extract. E inhibitors blocked both mycelial extract-induced and SA/MeJA-induced PR gene expression, and the cis pattern of responsiveness on the osmotin promoter was the same for the mycelial extract, SA, E, or E/MeJA. Seedlings treated with P. parasitica spores in the presence of SA/MeJA were protected from pathogen colonization. However, these seedlings exhibited symptoms of cell death (disease symptoms) both in the absence and presence of P. parasitica spores, in contrast to seedlings treated with nonpathogenic mycelial extract, which remained healthy. These results suggest that the signal transduction pathways for elicitation of defense responses by exogenously applied heat-stable nonpathogenic mycelial extract and SA/MeJA overlap at the point of PR protein induction but are not identical.
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  • 6
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We investigated the pathologic, bacteriologic and immunologic responses of BALB/c-nu/nu mice (nude mice) and BALB/c mice (euthymic mice) infected intravenously with virulent and avirulent Rhodococcus equi ATCC 33701, and its plasmid-cured derivative ATCC 33701P−, to evaluate the role of T lymphocytes. Adaptive transfer of immune and normal spleen cells into nude mice was also investigated. Nude and euthymic mice were inoculated with 106 ATCC 33701 or 106 ATCC 33701P− intravenously (i.v.) and killed at 0, 7, 14, 21, 28 and 35 days post-inoculation, except dead cases. In athymic nude mice infected with ATCC 33701, deteriorating systemic inflammatory responses developed during the experimental period and multiplication of the bacteria continued until the end of the experiment. Nude mice developed splenomegaly and multifocal gross hepatic necrosis with some mortality. Splenomegaly was caused by diffuse proliferation of bacteria-laden macrophages and epithelioid cells, and gross hepatic necrosis was caused by the formation of thromboses and granulomatous lesions. Infection of euthymic mice with a sublethal dose of ATCC 33701 resulted in transient granuloma formation in the liver and spleen, production of specific antibodies against the virulent bacteria and gradual elimination thereof. In contrast, infection with ATCC 33701P− produced few lesions after rapid elimination and no antibody production against bacteria in either normal or athymic nude mice. In nude mice given normal and immune spleen cells, histopathological lesions and granulomas formed only in the liver and spleen, in addition to specific antibodies against 15- to 17-kDa antigens. The pathological lesions observed in the nude mice given immune spleen cells were similar to those seen in the mice given normal spleen cells, but they were less severe than those in mice given normal spleen cells. Mice given immune spleen cells showed a significantly higher elevation of antibody production than mice given normal spleen cells. These results suggested that protection against virulent R. equi in mice depends mainly on cell-mediated immune responses, whereas avirulent R. equi in mice are cleared by innate immune responses.
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  • 7
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Auxin-binding protein (ABP1) is a putative receptor for auxin in the plasma membrane. We isolated a full-length cDNA encoding ABP1 from radish by screening a cDNA library with its partial cDNA fragment generated by the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Radish abpl mRNA was highly expressed in cotyledons, hooks and hypocotyls, and less so in roots of radish seedlings. The deduced amino acid sequence of radish abpl consisted of conserved auxin-binding motifs, a signal peptide and ER-retention signal, and contained two potentially N-linked glycosylation signals. To analyze the biochemical properties of the radish abpl product, this cDNA was expressed in insect cells by the baculovirus vector system. The result of tunicamycin-treatment showed that the signal peptide was cleaved and that the radish abpl product was glycosylated at both target sites in insect cells.
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  • 8
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Osmotin gene expression and protein synthesis were up-regulated in young tomato (Lycopersicon esculentum cv. Rheinlands Ruhm) plants after a short exposure (24 h) to 150 mM NaCl or 100 μM abscisic acid (ABA) or after dehydration to 80% of original plant fresh weight. Osmotically induced accumulation of osmotin mRNA was accompanied by a large increase in endogenous ABA levels. Increasing accumulation of osmotin protein was also observed during a longer exposure (7 days) to salt. Upon treatment with NaCl. osmotin mRNA levels increased in both root and leaf tissues. with an additional longer transcript induced in roots. No induction of osmotin mRNA was observed upon salt or water stress of the tomato ABA-deficient mutant sitiens. Treatment of sitiens with exogenous ABA induced osmotin mRNA accumulation to the level normally found in salt-treated wild-type plants. However, salt stress alone enhanced accumulation of osmotin mRNA in plants of another tomato mutant (flacca) which is also impaired in ABA synthesis. In tobacco plants carrying an osmotin promoter/β-glucuronidase (GUS) fusion gene, NaCl induction of GUS could be only partially blocked by the ABA inhibitor fluoridone. In flacca plants simultaneous treatment with NaCl and ABA resulted in higher levels of osmotin transcript compared to those following treatment with NaCl alone. No accumulation of osmotin protein was observed after short- or long-term osmotic treatments of the mutants. Our results support previous evidence that osmotin gene expression may be triggered in part through ABA and in part through a separate pathway of gene activation.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 95 (1995), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Arachidonic acid (AA), a fatty-acid fungal elicitor, and a cellulase preparation from Aspergillus niger, a protein-type fungal elicitor, induced osmotin gene expression. Both elicitors activated the osmotin promoter fused to a β-glucuronidase (GUS) reporter gene in a tissue-specific manner in tobacco seedlings (Nicotiana tabacum L. cv. Wisconsin 38). The cellulase preparation was more effective than AA at the concentrations tested and, unlike AA, also induced the accumulation of osmotin mRNA and protein. Combinations of AA and the cellulase preparation had a greater than additive effect on the activation of the osmotin promoter and the accumulation of osmotin mRNA and protein. Both AA and the cellulase preparation, when applied separately, were virtually ineffective in the induction of the osmotin promoter in cotyledon tissues. However, together they were able to induce synergistically GUS fused to the osmotin promoter. Increases in osmotin-promoter-driven GUS activity and accumulation of osmotin mRNA induced by AA, the cellulase preparation or their combination were reversed by norbornadiene, an ethylene action inhibitor, indicating that ethylene is involved in the induction of the osmotin gene by these elicitors.
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  • 10
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Germinating seeds of many species contain two types of β-cyanoalanine synthase (CAS, EC 4.4.1.9) that convert HCN to β-cyanoalanine. One is cytoplasmic CAS (cyt-CAS), which is precipitated by 50 to 60% (NH4)2SO4 and has a pH optimum of 10.5. Cytoplasmic CAS is present at high levels in dry seed and its activity does not increase during imbibition. The activity of cyt-CAS is not affected by exogenously applied ethylene (C2H4), except in rice (Oryza sativa cv. Sasanishiki). The second type of CAS found in seed is mitochondrial CAS (mit-CAS), which is precipitated by 60 to 70% (NH4)2SO4 and has a pH optimum of 9.5. Mitochondrial CAS is present at low levels in dry seed, and its activity increases greatly during imbibition in the seeds of all species tested. Exposure to C2H4 stimulated mit-CAS activity in seeds of rice, barley (Hordeum vulgare cv. Hadakamugi). cucumber (Cucumis sativus cv. Kagafushinari) and cocklebur (Xanthium pennsylvanicum). The increase in the mit-CAS activity in cocklebur in response to C2H4 commenced alter a lag period of 2 to 3 h when the duration of soaking was short (16 h), but commenced without a lag period when the seeds were soaked for three months. Application of both chloramphenicol and cycloheximide to the axial and cotyledonary tissues of cocklebur seeds strongly inhibited growth as well as the increase in mit-CAS activity. It is postulated that the mit-CAS is synthesized de novo during imbibition and that its activity is regulated by C2H4, CO2 which also promotes seed germination in some species, was ineffective m stimulating mit-CAS activity in cocklebur seeds.
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