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  • Springer  (18)
  • Oxford University Press  (5)
  • Blackwell Publishing Ltd  (2)
  • American Association for the Advancement of Science (AAAS)
  • BioMed Central
  • Geological Society of Japan
  • Wiley-Blackwell
  • 2020-2022
  • 1995-1999  (25)
  • 1970-1974
  • 1940-1944
  • 1996  (14)
  • 1995  (11)
Collection
Publisher
Years
  • 2020-2022
  • 1995-1999  (25)
  • 1970-1974
  • 1940-1944
Year
  • 1
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Mouse monoclonal antibody (mAb) Pgf-II specific for a 72-kDa major cell-surface protein (72K-CSP) derived from Porphyromonas gingivalis OMZ 409 was prepared. Immunoblotting analysis revealed that mAb Pgf-II reacted with 72K-CSP but not with 41-kDa fimbrial subunit protein (41K-fimbrilin) derived from P. gingivalis 381. Electron microscopic observation revealed that P. gingivalis OMZ 409 possessed peritrichous, thin fimbriae on their surface. Immunogold electron microscopy also demonstrated that mAb Pgf-II bound to the 72K-CSP examined with the gold particles arranged along the fibril array originating from the cell surface of the bacteria. These findings suggested that P. gingivalis 72K-CSP was identifiable as another fimbriae (termed Pg-II fimbriae) different from the fimbriae (termed Pg-I fimbriae) composed of a 41K-fimbrilin. Using multipin peptide synthesis technology, 102 sequential overlapping peptides covering the entire 514 amino-acid stretch of Pg-II fimbriae were synthesised. Seven immunodominant regions within Pg-II fimbrial protein molecule, which definitely reacted with the serum of patients with periodontal diseases, were detected.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract We previously established murine hybridomas producing a monoclonal antibody monospecific against three glucosyl-transferases (I, SI and S) of Streptococcus mutans which contribute to dental caries formation. Here, we developed a new immunochemical technique (cross-dot system) with which individual levels of glucosyltransferases expressed by S. mutans can be evaluated. We also examined glucosyltransferase production and in vitro artificial plaque formation by a reference strain and several clinical isolates of S. mutans. The findings indicate that the levels of glucosyltransferases produced greatly vary with the cells and the culture medium, and that the cells producing high levels of both glucosyltransferase-SI and glucosyltransferase-I enzymes may possess high in vitro artificial plaque forming ability. We suggest that the cross-dot system will be useful for estimating the cariogenic potential of S. mutans isolates.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Hyperfine interactions 97-98 (1996), S. 125-132 
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We present results of PAC measurements in heavy-fermion compound Yb4As3. The quadrupole hyperfine interaction has been investigated over the temperature range 78–850 K. The interpretation of the experimental data yields a microscopic proof of the existence of a charge-ordered state with periodic arrangement of Yb2+ and Yb3+ ions in the low temperature phase. In the high temperature phase all Yb ions are in a valence fluctuating state.
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  • 4
    ISSN: 1618-0860
    Keywords: Cell-cell adhesion ; Cellular slime mold ; Glycoprotein ; Polysphondylium pallidum ; Tunicamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To assess the role in cell-cell adhesion of gp64, a putative cell-cell adhesion molecule ofPolysphondylium pallidum, we treated the cells with tunicamycin (TM), a known inhibitor of the synthesis of the N-linked oligosaccharide precursor, and examined TM's effect on cell-cell adhesion. The vegetative growth ofPolysphondylium cells was inhibited with TM in a dose-dependent manner. When cells were treated with TM (2.0 μg/ml) during only the first 4 hr of starvation and further starved for 8 hr without TM, the cells dissociated considerably, although even the growth phase cells ofPolysphondylium normally show EDTA-resistant (Ca2+-independent) cell adhesions. In parallel with the above effects, the amounts of intact gp64 decreased considerably in time with the lengths of incubation (0 hr〉4 hr 〉8 hr). When TM-treated cells were washed free of TM, and shaken for a further 12 hr, the cells began to aggregate again, accompanied by an increase of gp64. In conclusion, TM affected cell-cell adhesion ofPolysphondylium cells, but we were not able to distinguish whether the inhibition of cell aggregation was due to defects in glycosylation on glycoproteins and/or due to reduced levels of glycoproteins themselves.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Ecological research 11 (1996), S. 89-95 
    ISSN: 1440-1703
    Keywords: age variation ; Cervus nippon ; food habits ; local variation ; point-frame methods ; sexual variation ; sika deer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rumen contents of sika deer (Cervus nippon Temminck) on the Boso Peninsula, central Japan, were analyzed to identify local, sexual and age-specific differences in food habits. Graminoids and woody plants were the primary foods throughout the year. In winter, the use of evergreen broad leaves increased. The food habits of sika deer on Boso Peninsula were intermediate between those of populations inhabiting northern and southern Japan. Acorns, mainlyLithocarpus edulis Nakai, were consumed in fall and winter with a peak in October. Since the availability of acorns is not influenced by foraging in previous years, it can be regarded as a stable food supply and hence may be important for deer on the Boso Peninsula. The local difference between the Amatsukominato (AT) area, having a large plantation ofLithocarpus producing acrons, and the Kamogawa-Katsuura (KK) area, having a small plantation ofLithocarpus, was recognized; seeds and fruit were consumed more in AT than in KK in fall and winter. Males consumed more seeds and fruit than females at both sites in fall. This can be attributed to sexual differences in nutritional requirement.
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  • 6
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A useful method to calculate stress disturbances arising from cut fibres is the so-called shear lag analysis, in which it is assumed that fibres act to carry tensile stress without transferring applied stress to the matrix, and the matrix acts to transfer stress to the fibres without carrying tensile stress. This assumption gives a limit for application. In the present work, with unidirectional metal matrix composites in mind, the usual two-dimensional shear lag analysis was modified to express the situation where both fibres and matrix act to carry applied stress and also to transfer stress. By using this modified method, tensile strain concentration in the fibres and matrix adjacent to cut fibres and matrix, and shear stresses at the interface between fibres and matrix, were calculated for some examples.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 30 (1995), S. 274-279 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A calculation method to describe the influence of the non-uniform crack-spacing in a strongly adhering coating layer, which shows multiple cracking, on the energy release rate of a coated fibre is presented and applied to some examples. Four main results were found. The energy release rate of the fibre at the crack in the region of narrow crack-spacing was low. For any crack-spacing, the energy release rate was high when the Young's modulus of the fibre was low and that of the coating layer was high. From the calculation of energy release rates of the fibre at all cracks, the strength-determining crack could be identified, and from this the fibre strength after multiple cracking of the coating layer could be estimated. Finally, the strength of the fibre for non-uniform crack spacing was lower than that for a uniform one.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 30 (1995), S. 509-513 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Multiple cracking of a coating layer and its influence on the tensile strength of the coated fibre in the case of strong interfacial bonding were simulated by means of a Monte Carlo method. Within the range where the coating layer was weak, it was found that the weaker the coating layer, the larger became the number of cracks and consequently the smaller became the crack spacing, resulting in higher strength of the fibre. When the strength of the layer was high, however, the crack spacing became large, resulting in a low strength of the fibre comparable to the strength for the case of single cracking. The strength value of the fibre calculated for average crack spacing gave an upper bound, and that calculated for single cracking a lower bound, for the actual fibre strength.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 31 (1996), S. 2027-2034 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In order to predict the effect of small-scale interfacial debonding on the energy release rate at a crack tip for mode I fracture of a fibre with a cracked coating layer, an approximate calculation method has been presented. The relation of debonding length, thickness of the coating layer and ratio of elastic modulus of the coating layer to that of the fibre, to the energy release rate of the fibre was calculated for some examples. It was demonstrated that small-scale debonding reduces the energy release rate and, therefore, effectively prevents reduction in fibre strength.
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  • 10
    ISSN: 1435-232X
    Keywords: X-linked α-thalassemia/mental retardation syndrome (ATR-X) ; HbH inclusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary X-linked α-thalassemia/mental retardation syndrome (ATR-X) is characterized by severe mental retardation, wide range of minor abnormalities, and association with an unusual form of α-thalassemia. Fifty patients in Caucasian origin have been reported. This is the second report of the syndrome demonstrated in Oriental patients.
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