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  • Chemistry  (19)
  • Ammonia metabolism  (2)
  • pearl millet  (2)
  • Computational Chemistry and Molecular Modeling
  • sorghum
  • 1990-1994  (24)
  • 1994  (24)
Collection
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  • 1990-1994  (24)
Year
  • 1
    ISSN: 1573-5087
    Keywords: coleoptile length ; dwarf mutants ; gibberellin levels ; gibberellin sensitivity ; pearl millet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The concentrations of endogenous gibberellins (GAs) were determined by combined gas chromatography-mass spectrometry in shoots of five non-allelic dwarfs of pearl millet Pennisetum glaucum (L.) R. Br. One mutant (d3), with an extreme dwarf phenotype, was found to be deficient in all GAs measured; the others (d1, d2, d4 and the quantitatively inherited dwarf) had similar levels of GAs to the tall genotype. Only the GA-deficient dwarf recovered the tall phenotype in response to applying GA3 up to the adult stage, while the others showed slight to moderate responses at the seedling stage, depending on the season, and no response at later stages. The d1, d3 and d4 dwarfs had short coleoptiles. A wide range of coleoptile lengths with a normal distribution pattern was observed in the tall, d2 and the quantitatively inherited dwarf, suggesting that there is polygenic control of this trait.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 15 (1994), S. 907-910 
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A method is described for the direct detection of lectins, agglutinating erythrocytes, on nitrocellulose membranes after Western blotting, thus avoiding protein extraction from specific bands in the gel, followed by agglutination assays. The methodology essentially involves exposing the lectin band on a nitrocellulose strip to trypsinized rabbit erythrocytes (2%, in 0.15 M NaCl) for 30 min at 37°C and then carefully transferring the membrane to saline (4°C) for a few gentle washes and then fixing it in a solution (0.2% glutaraldehyde in 0.15 M NaCl) for 30 min. Later, the membrane is gently washed several times in 0.15 M NaCl containing 10 mM β-alanine. The lectin band is visualized as a red agglutinated patch. The method is specific for lectins that can agglutinate red blood cells and virtually has no cross reactivity with the various nonlectin proteins tested. Binding of erythrocytes to the lectin band on the nitrocellulose strip can be prevented by specific competing sugars. The method can be applied to screen for the presence of lectins in natural materials and to monitor lectin fractions during purification.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 162 (1994), S. 282-285 
    ISSN: 1432-072X
    Keywords: Key words     Cyanobacteria (Phormidium uncinatum) ; Oxidation and dismutation of nitrogen compounds ; Oxime metabolism ; Hydrogen peroxide production ; Ammonia metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      Ammonia-incubated cyanobacteria liberated H2O2, accumulated hydroxylamine compounds and nitrite and catalyzed dismutation of hydroxylamine as well as oxidations of ammonia, glutamine, and oximes. Ethyl acetohydroximate-adapted Phormidium released excess H2O2 and phototrophically metabolized the oxime via hydrolysis and dismutation to nitrite and ammonia, which were co nsumed by nitrite reductase and glutamine synthetase. Added ammonia stimulated H2O2 production and oxime metabolism via glutamate dehydrogenase pathway.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 162 (1994), S. 282-285 
    ISSN: 1432-072X
    Keywords: Cyanobacteria (Phormidium uncinatum) ; Oxidation and dismutation of nitrogen compounds ; Oxime metabolism ; Hydrogen peroxide production ; Ammonia metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ammonia-incubated cyanobacteria liberated H2O2, accumulated hydroxylamine compounds and nitrite and catalyzed dismutation of hydroxylamine as well as oxidations of ammonia, glutamine, and oximes. Ethyl acetohydroximate-adapted Phormidium released excess H2O2 and phototrophically metabolized the oxime via hydrolysis and dismutation to nitrite and ammonia, which were consumed by nitrite reductase and glutamine synthetase. Added ammonia stimulated H2O2 production and oxime metabolism via glutamate dehydrogenase pathway.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5060
    Keywords: Pennisetum glaucum ; pearl millet ; landrace cultivars ; topcross hybrids ; grain yield ; downy mildew ; renstance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pearl millet (Pennisetum glaucum (L.) R. Br.) cultivars for marginal, arid environments need to combine the adaptation to stress conditions of indigenous landraces with an improved yield potential and disease resistance, to allow them to both perform well in farmers fields and to meet the requirements for cultivar release. This paper evaluates landrace-based topcross hybrids (adapted landraces crossed on high-yielding male-sterile lines), as a quick and efficient way of achieving this objective. Topcross hybrids showed a consistent increase in biomass production across all test environments, including the harsh arid zone environments. Depending upon the plant type of the male-sterile used to make the hybrid, this was expressed as increased grain yield only, or increased grain and fodder yields. The downy mildew (Sclerospora graminicola) reaction of the topcross hybrids was determined by the reaction of the male-sterile line used, with the resistant male-sterile producing resistant topcross hybrids and vice-versa. Topcrossing adapted landraces on high-yielding male-sterile lines thus provides an opportunity to improve disease resistance and grain and/or fodder yields, with no apparent loss of adaptation to the marginal environments in which the landraces have evolved.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 874-880 
    ISSN: 0006-3592
    Keywords: sludge ; sorption ; precipitation ; metals ; adsorption ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A conceptual model describing the relative roles of sorption and precipitation processes for metals in solid-solution suspensions is presented. The model performance is demonstrated using experimental data on sorption and precipitation of metals in samples of activated sludge mixed liquor. Based on the experimental results presented here, it appears that, at total metal and mixed liquor suspended solids concentrations and pH values generally encountered in full-scale municipal (or combined municipal/industrial) activated sludge systems, metals are primarily removed by sorption processes.
    Additional Material: 7 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 453-459 
    ISSN: 0006-3592
    Keywords: NAD(P)H fluorescene ; on-line monitoring ; amino acid fermentation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The amino acid fermentation by Corynebacterium glutamicum was monitored with an new technique that uses the first derivative of the NAD(P)H fluorescene signal. The rate of change of NAD(P)H pools is indicative of intracellular redox balance variations that correspond to metabolic changes. The profile of this signal showed several characteristics that coincided with major metabolic events during fermentation. We show here that the derivative fluorescence signal can accurately estimate points of threonine depletion, viable cell count, and the end of amino acid formation. Furthermore, on-line optimization strategies can be developed by using the derivative fluorescene signal. © 1994 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 1139-1145 
    ISSN: 0006-3592
    Keywords: fluorescence lifetime ; phase modulation fluorometry ; dissolved oxygen tension ; oxygen measurement ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We report here on a low-cost, optical oxygen sensor as an attractive alternative to the widely used amperometric Clark-type oxygen electrode for measuring dissolved oxygen tensions in cell cultures and bioreactor. Our sensor is based on the defferential quenching of the fluorescence lifetime of chromophore in response to the partial pressure of oxygen. This is measured as a phase shift in fluorescence emission from the chromophore due to oxygen quenching when excited by an intensity modulated beam of light. In this article we demonstrate the advantages of lifetime-based optical methods over both intensity based optical methods and amperometric electrodes. Our sensor is particularly suitable for measuring dissolved oxygen in bioreactors. It is autoclavable, is free of maintenance requirements, and solvents the problems of long-term stability, calibration drifts, and reliable measurement of low oxygen tensions in dense microbial cultures that limit the utility of Clark-type elcectordes. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 17 (1994), S. 561-562 
    ISSN: 0935-6304
    Keywords: Reversed phase LC ; Ion pair LC ; Quality assurance ; 2,5-Diaminobenzene-1,4-disulfonic acid ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 10
    ISSN: 0887-6266
    Keywords: phthalocyanine polymers ; electrical conductivity ; magnetic susceptibility ; electron spin resonance ; soliton defects ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Polymeric metallophthalocyanines of Cu, Ni, Co in which benzene rings are shared in common with the macrocyclic phthalocyanine rings containing peripheral carboxylic groups have been synthesized and their electrical conductivities are shown to increase from 5 to 8 orders of magnitude by thermal treatment. The cobalt polymer exhibits greater conductivity than its nickel or copper analogs. The heated polymers show very broad electron spin resonance (ESR) signals and large paramagnetic susceptibilities. The bulk magnetic susceptibility of these polymers shows Curie-like behavior when the samples are heated from room temperature to 473 K. This is explained on the basis of coexistence of fixed mobile and conduction electron spins in the system and the interaction of the soliton type defects present in these polymers with the polymeric network to give dipolar charge carriers. This is also supported by the decrease in ESR intensity with increasing temperature when the samples are heated in the ESR cavity. The effect of the presence of unpaired electrons on the d shell of the central metal atoms on the line width and the line shape of the ESR spectra is also explained. © 1994 John Wiley & Sons, Inc.
    Additional Material: 18 Ill.
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