ISSN:
1617-4623
Keywords:
Drosophila melanogaster
;
Transcription map
;
Faint bands
;
Interband chromatin
;
Polytene chromosomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Urate oxidase mRNA and five other transcripts map along 38 kb of DNA in the region 28C on the Drosophila melanogaster second chromosome. Three biotinylated restriction fragments from this 38 kb of DNA, one from each end and one from the middle, were individually hybridized in situ to slightly stretched salivary gland polytene chromosomes. The data from these in situ hybridizations in combination with the transcription map of the 38 kb of DNA indicate that: (i) there are six discrete RNA species encoded along the 38 kb of DNA and (ii) these six transcripts map to the faint band/interband region which includes the proximal edge of 280, the three faint bands, 28C2, 280 and 28C4-5(−), and the adjacent interband chromatin. Our data are consistent with the few published studies directly demonstrating that faint band/interband regions of the Drosophila melanogaster salivary gland polytene chromosomes code for a high density of transcripts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273590
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