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The faint band/interband region 28C2 to 28C4-5(−) of the Drosophila melanogaster salivary gland polytene chromosomes is rich in transcripts

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Summary

Urate oxidase mRNA and five other transcripts map along 38 kb of DNA in the region 28C on the Drosophila melanogaster second chromosome. Three biotinylated restriction fragments from this 38 kb of DNA, one from each end and one from the middle, were individually hybridized in situ to slightly stretched salivary gland polytene chromosomes. The data from these in situ hybridizations in combination with the transcription map of the 38 kb of DNA indicate that: (i) there are six discrete RNA species encoded along the 38 kb of DNA and (ii) these six transcripts map to the faint band/interband region which includes the proximal edge of 280, the three faint bands, 28C2, 280 and 28C4-5(−), and the adjacent interband chromatin. Our data are consistent with the few published studies directly demonstrating that faint band/interband regions of the Drosophila melanogaster salivary gland polytene chromosomes code for a high density of transcripts.

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Author notes

  1. Kelly N. Owens

    Present address: Department of Genetics, SK50, University of Washington, 98195, Seattle, WA, USA

Authors and Affiliations

  1. Graduate Program in Genetics, Michigan State University, 48824, East Lansing, MI, USA

    Thomas B. Friedman & Lori L. Wallrath

  2. Cell and Molecular Biology Program, Michigan State University, 48824, East Lansing, MI, USA

    Lori L. Wallrath

  3. Department of Zoology, Michigan State University, 48824, East Lansing, MI, USA

    Kelly N. Owens & Jean B. Burnett

  4. College of Human Medicine, Michigan State University, 48824, East Lansing, MI, USA

    Thomas B. Friedman

  5. Department of Genetics, University of Helsinki, Arkadiankatu 7, Finland

    Anja O. Saura

Authors
  1. Thomas B. Friedman
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  2. Kelly N. Owens
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  3. Jean B. Burnett
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  4. Anja O. Saura
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  5. Lori L. Wallrath
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Communicated by B.H. Judd

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Friedman, T.B., Owens, K.N., Burnett, J.B. et al. The faint band/interband region 28C2 to 28C4-5(−) of the Drosophila melanogaster salivary gland polytene chromosomes is rich in transcripts. Mol Gen Genet 226, 81–87 (1991). https://doi.org/10.1007/BF00273590

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  • DOI: https://doi.org/10.1007/BF00273590

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