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  • Articles  (12)
  • Life and Medical Sciences  (12)
  • SOLAR PHYSICS
  • 1985-1989  (12)
  • 1980-1984
  • 1940-1944
  • 1915-1919
  • 1989  (12)
  • Natural Sciences in General  (12)
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  • Articles  (12)
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  • 1985-1989  (12)
  • 1980-1984
  • 1940-1944
  • 1915-1919
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    An inexpensive detector for water-vapour in the low temperature scanning electron microscope (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 11 (1989), S. 90-91 
    Details
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060110112
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  • 2
    Electronic Resource
    Electronic Resource
    Optimum specimen positioning in the electron microscope using a double-tilt stage (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 11 (1989), S. 33-40 
    Details
    ISSN: 0741-0581
    Keywords: High-voltage electron microscopy ; Thick specimens ; Tomography ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Optimal imaging of complex structures requires proper alignment relative to the optic axis of the electron microscope. This is especially important for high-voltage and intermediatevoltage microscopes, which form an in-focus image throughout the entire thickness of the object. As a result, structures at different specimen heights form overlapping and confused images that severely curtail the usefulness of these instruments.The work described here provides a generalized, flexible method for optimizing specimen orientation and eliminating or limiting image overlap by means of a commonly used double-tilt stage. Analysis of the motion about the two axes provides accurate tilting for any azimuthal direction whether or not it corresponds to a mechanical axis of the stage. An object can be positioned to minimize image overlap, to record stereopairs for any parallax axis, and to record three-dimensional data sets by the conical collection geometry.Images of muscle paracrystals are shown after tilting about an axis perpendicular to a symmetry direction. The tilted image displays higher-order symmetry, which is altered by changes of one degree. Precision double-tilting for optimizing stereopairs is shown for a desmosome recorded using different parallax axes and pretilts. A tomographic conical data-collection scheme is demonstrated by imaging a microtubule axoneme for a specific cone half-angle and arbitrary azimuthal angles.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060110106
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  • 3
    Electronic Resource
    Electronic Resource
    Direct observation of molecular images of lanthanide phthalocyanines: III. Structural defects (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 11 (1989), S. 326-332 
    Details
    ISSN: 0741-0581
    Keywords: Organic crystal defects ; High-resolution electron microscopy ; Thin epitaxial organic films ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The crystal imperfections in thin films of lanthanide phthalocyanines (LnPc2H, Ln = Nd, Tb, Er, Tm, Yb, and Lu) grown epitaxially on KCl have been ovserved by molecular imaging. Grain and twin boundaries, stacking faults, point defects, vacancies, mosaic structures, and sometimes even some amorphous islands exist in the well-crystallized specimens. Combined with the results reported earlier, the packing characteristics of planar LnPc2H molecules can be well understood.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060110411
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  • 4
    Electronic Resource
    Electronic Resource
    Preface (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 307-307 
    Details
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120402
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  • 5
    Electronic Resource
    Electronic Resource
    Adrenal chromaffin cells as transplants in animal models of parkinson's disease (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 308-315 
    Details
    ISSN: 0741-0581
    Keywords: Adrenal medulla ; Electron microscopy ; Transplantation ; Plasticity ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The field of neural transplantation has moved rapidly forward in the last decade. Initially, fetal cells were used as implants to investigate their potential to ameliorate deficits in animal models of Parkinson's disease. However, because of the moral and legal problems associated with the use of fetal tissues in humans, alternative sources of donor tissue were sought which possessed the structural and functional characteristics needed to improve motor function in Parkinsonian patients. To date, one of the most promising tissues being investigated is the adrenal medulla, whose chromaffin cells possess an inherent plasticity of form and function. Transplanted chromaffin cells currently are being studied by a variety of approaches, including electron microscopy, in mouse, rat, and primate models of Parkinson's disease. An overview of the role of the chromaffin cell in this exciting and clinically important arena is briefly reviewed, with an emphasis on the fine structure of implanted chromaffin cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120403
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural demonstration of exocytosis in the intact rat adrenal medulla (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 316-322 
    Details
    ISSN: 0741-0581
    Keywords: Secretion ; Electron microscopy ; Tannic acid ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Evidence is presented for morphological proof of exocytosis in the rat adrenal medulla in situ. Techniques were modified to allow perfusion of the intact adrenal gland with secretagogues (or electrical stimulation) followed by tannic acid. Unstimulated specimens demonstrated exocytotic (omega-shaped) profiles filled with flocculent material. This flocculation was also seen in the intercellular space. Stimulation of the adrenal medulla also resulted in the appearance of exocytotic profiles and an accumulation of the flocculent mass. This was often most evident in the subendothelial space. This is the first demonstration of exocytosis in the rat adrenal medulla by electron microscopy. The techniques used in this study will be useful for studying the pathway of secretory products of the adrenal chromaffin cell before they enter the vascular system.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120404
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  • 7
    Electronic Resource
    Electronic Resource
    Mitotic cell division in the extraadrenal chromaffin system of various species (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 323-330 
    Details
    ISSN: 0741-0581
    Keywords: Chromaffin cells ; Paraganglia ; Paraaortic organs ; Mitosis ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Mitotic activity often has been reported in embryonic and fetal sympathetic neuroblasts, principal sympathoblasts, and primitive sympathetic cells in various species at different stages of development. Postnatal adrenal medullary cells also are known to undergo mitosis, but such dividing capabilities rarely have been observed in the true postnatal extraadrenal chromaffin system. Although few in number, this work nevertheless has clearly identified such cells in varying stages of the mitotic cycle in the young dog, Syrian hamster, mouse, rabbit, and rat. The dividing cells were noted in paraaortic chromaffin organs, paraganglia, and within the inferior mesenteric ganglion as well. They displayed the morphological character usually associated with their adrenal medullary catecholaminergic counterparts, including numerous dense-cored vesicles known to be the harbingers of catecholamines and various peptides. Nerve endings were not noticed upon the mitotic cells. The phenomenon of dividing extraadrenal chromaffin cells augments existing data and perhaps suggests that these cells are more endocrine than neural in type and subservient to the adrenal medulla in its classic endocrine function.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120405
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  • 8
    Electronic Resource
    Electronic Resource
    Applications of convergent beam electron diffraction in materials science (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 13 (1989), S. 66-75 
    Details
    ISSN: 0741-0581
    Keywords: Symmetry determination ; Space-group ; Point-group ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The methods by which Convergent Beam Electron Diffraction (CBED) is used to solve practical problems in materials science are reviewed. Particular emphasis is placed on those applications where a consensus has developed on the potential difficulties, but these problems have never been explicitly treated in the literature. Point-group determination using CBED is a standard technique, but there are several different experimental methods which are outlined here; the different approaches and the information obtained are compared. These point-symmetry determinations are shown to rely heavily on elimating the large number of potential artefacts: the possible sources of symmetry-breaking are summarised, and their salient features are identified. This should give a prescription for demonstrating whether symmetry-breaking arises through crystal structure, defects, strain, or specimen morphology. In space-group determinations the practical approach is now fairly standard, but the article draws attention to some anomalies. Strain and lattice parameter determinations are also standard, but a consensus is beginnning to emerge that the interpretive difficulties involved in allowing for thin film relaxation are extremely severe: a few of these difficulties are outlined. Finally, the article also addresses some of the techniques which have been used to extract structural or compositional information from CBED: the experimental approach depends on the specific problem, and the article aims to provide some examples to illustrate what is possible.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060130109
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  • 9
    Electronic Resource
    Electronic Resource
    Convergent beam diffraction studies of interfaces, defects, and multilayers (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 13 (1989), S. 111-122 
    Details
    ISSN: 0741-0581
    Keywords: Crystal defects ; Bicrystals ; Layer strains ; Large angle convergent beam electron diffraction ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: This paper explains how the convergent beam electron diffraction (CBED) and large angle convergent beam (LACBED) techniques can be used to study crystal defects, bicrystals, and multilayers. It is shown how the LACBED technique in particular can be used to derive the magnitude and sign of the Burgers vectors of dislocations and displacements at stacking faults. For bicrystals and multilayers examined in plan-view, LACBED gives the rocking curve for a chosen reflection. This enables layer strains to be measured to ∼ 0.1% and composition profiles derived for both periodic and aperiodic structures. It is shown that a simple kinematic approach can be used to interpret the essential results in most cases.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060130204
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  • 10
    Electronic Resource
    Electronic Resource
    A flow-through staining system for use with multiple em grids (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 13 (1989), S. 154-155 
    Details
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060130208
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