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  • 2000-2004  (18)
  • 1990-1994
  • 1985-1989  (28)
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  • 2004  (18)
  • 1989  (28)
  • 1
    Electronic Resource
    Electronic Resource
    Dynamics of the triplet state and the reverse proton transfer of 3-hydroxyflavone (1989)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 93 (1989), S. 6088-6094 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100353a029
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  • 2
    Electronic Resource
    Electronic Resource
    Time-resolved study of the photooxygenation of 3-hydroxyflavone (1989)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 111 (1989), S. 7643-7644 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00201a071
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  • 3
    Electronic Resource
    Electronic Resource
    A nanoliter-scale nucleic acid processor with parallel architecture (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 435-439 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The purification of nucleic acids from microbial and mammalian cells is a crucial step in many biological and medical applications. We have developed microfluidic chips for automated nucleic acid purification from small numbers of bacterial or mammalian cells. All processes, such as cell isolation, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt951
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  • 4
    Electronic Resource
    Electronic Resource
    Tetragonal-to-Monoclinic Transformation in Mg-PSZ Studied by in Situ Neutron Diffraction (2004)
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 87 (2004), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The deformation of 9.4 mol% magnesia-partially-stabilized zirconia under compressive loads up to 1225 MPa was studied using mechanical testing with in situ neutron diffraction. The material shows obvious plastic deformation at applied stresses in excess of an estimated critical stress of 925 ± 20 MPa. Most of the accumulated strain occurred by transient room-temperature creep. Plastic deformation was associated with considerable stress-induced tetragonal-to-monoclinic transformation. The volume change calculated from the strain gauges correlates well with the amount of t→m transformation observed. Unlike previous studies of Ce-TZP and Y-TZP, ferroelasticity was not observed, nor was the t→o transformation observed. Minor microstructural changes were noted, including an increase in the root mean square internal strain of 0.05%, commensurate with an increase in internal stress of ∼100 MPa. It would appear that transformation selectivity was exercised with the transformation occurring first in tetragonal crystallites favorably oriented to the applied stress. The stress-induced monoclinic phase therefore exhibits a strong preferred orientation. Comparison is made with the other commercially interesting zirconia ceramics, Ce-TZP and Y-TZP, which have been studied using the same techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1551-2916.2004.00465.x
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  • 5
    Electronic Resource
    Electronic Resource
    Specific amplification of the ZFY gene to screen sex in man (1989)
    Springer
    Human genetics 〈Berlin〉 82 (1989), S. 289-290 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the polymerase chain reaction, a sequence comprising 400 bp of the human ZFY gene was amplified specifically in the male. The method allows detection of the presence of the ZFY gene in the order of 1:104 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291174
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of patatin, the major glycoprotein in potato (Solanum tuberosum L.) tubers (1989)
    Springer
    Planta 178 (1989), S. 176-183 
    Details
    ISSN: 1432-2048
    Keywords: Glycoprotein ; Lipid acyl hydrolyse ; Patatin (immunocytochemical localization) ; Solanum (patatin localization) ; Vacuole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Patatin is a family of glycoproteins with an apparent molecular weight of 40 kDa. The protein is synthesized as a pre-protein with a hydrophobic signal sequence of 23 amino acids. Using different immunocytochemical methods we determined the tissue-specific as well as subcellular localization of the patatin protein. Since antibodies raised against patatin showed crossreactivity with glycans of other glycoproteins, antibodies specific for the protein portion of the glycoprotein were purified. Using these antibodies for electron-microscopical immunocytochemistry, the protein was found to be localized mainly in the vacuoles of both tubers and leaves of potatoes (Solanum tuberosum L.) induced for patatin expression. Neither cell walls nor the intercellular space contained detectable levels of patatin protein. Concerning the tissue specificity, patatin was mainly found in parenchyma cells of potato tubers. The same distribution was observed for the esterase activity in potato tubers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393192
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  • 7
    Electronic Resource
    Electronic Resource
    Cloning of a DNA region from Bradyrhizobium japonicum encoding pleiotropic functions in heme metabolism and respiration (1989)
    Springer
    Archives of microbiology 151 (1989), S. 203-212 
    Details
    ISSN: 1432-072X
    Keywords: Bradyrhizobium ; Gene cloning ; Heme ; Marker exchange mutagenesis ; Nitrogen fixation ; Respiration ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Random and site-directed Tn5-induced mutagenesis of Bradyrhizobium japonicum yielded two mutations, one in strain 2960 and the other in strain 2606::Tn5-20, which mapped close to each other but in separate genes. The corresponding wild-type genes were cloned, and their approximate location on the cloned DNA was determined. Mutant 2960 was Fix- and formed green nodules on soybean, whereas strain 2606::Tn5-20 had ca. 4% of wild-type Fix activity and formed white nodules. Cytochrome oxidase assays (Nadi tests) showed a negative reaction with both mutants, indicating a functional deficiency of cytochrome c or its terminal oxidase or both. However, the mutants grew well under aerobic conditions on minimal media with different carbon sources. Furthermore, mutant 2960 had a reduced activity in hydrogen uptake, was unable to grow anaerobically with nitrate as the terminal electron acceptor and 2960-infected soybean nodules contained little, if any, functional leghemoglobin. Southern blot analysis showed that a B. japonicum heme biosynthesis mutant [strain LO505: O'Brian MR, Kirshbom PM, Maier RJ (1987) Proc Natl Acad Sci USA 84: 8390–8393] had its mutation close to the Tn5 insertion site of our mutant 2606::Tn5-20. This finding, combined with the observed phenotypes, suggested that the genes affected in mutants 2960 and 2606::Tn5-20 were involved in some steps of heme biosynthesis thus explaining the pleiotropic respiratory deficiencies of the mutants. Similar to strain LO505, the mutant 2606::Tn5-20 (but not 2960) was defective in the activity of protoporphyrinogen IX oxidase which catalyzes the penultimate step in the heme biosynthesis pathway. This suggests that one of the two cloned genes may code for this enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413131
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of ψM1, a virulent phage of Methanobacterium thermoautotrophicum Marburg (1989)
    Springer
    Archives of microbiology 152 (1989), S. 105-110 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Bacteriophage ; Methanogenic bacteria ; Plasmid pME2001
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacteriophage ψM1, a virulent, oxygen resistant phage of Methanobacterium thermoautotrophicum strain Marburg, was isolated from an anaerobic sludge digester operated at 55°C to 60°C. A reproducible plaque assay and an enrichment procedure for the preparation of high-titer lysates (2x1010 PFU/ml) were established. One-step growth experiments at 62°C showed that the latent period was 4 h and the burst size was 5–6 infective particles per cell. The phage infected Methanobacterium thermoautotrophicum Marburg but none of three other thermophilic representatives of the genus Methanobacterium that were tested. Electron micrographs showed that phage ψM1 has a polyhedral head of 55 nm diameter and a tail of 210 nm in lenght. The ψM1 genome consists of linear double-stranded DNA with a size of 30.4±1.0 kb. Restriction and hybridization analysis of DNA extracted from phage particles revealed two types of linear molecules with the size of the phage genome. About 85% of the DNA molecules in such preparations were genomes of ψM1 whereas approximately 15% were multimers of the cryptic 4.5-kb plasmid pME2001 of the host. ψM1 DNA did not hybridize with chromosomal DNA of Methanobacterium thermoautotrophicum but it exhibited definite homology to total DNA of Methanobacterium wolfei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00456085
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  • 9
    Electronic Resource
    Electronic Resource
    Metal Complexes with Macrocyclic Ligands. Part XXVIII.Part XXVII: [1]. Structures and stabilities of the Cu2+ complexes with 1,4,7-triazacyclononane-1-acetic acid (1989)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 72 (1989), S. 307-312 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The potentiometric study of the complexation of 1,4,7-triazacyclononane-l-acetic acid (1) with Cu2+ (I = 0.5 (KNO3), T = 25°) indicates the presence of the species [Cu(1)], [Cu(1)OH], [Cu(1)2], and [(Cu(1))2OH], the stability constants of which are determined. The two complexes [Cu(1)]ClO4 and [(Cu(1)2)OH]ClO4 were also characterized by X-ray structure analysis. In both cases, the Cu2+ ion is in a distorted square-pyramidal arrangement, penta-coordinated by the three N-atoms of the macrocycle, an O-atom of the carboxylate, and an additional O-atom either from a second carboxylate or from an OH-, acting as a bridge between two metal centres.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19890720215
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  • 10
    Electronic Resource
    Electronic Resource
    Metal Complexes with Macrocyclic Ligands Part XXXPart XXIX, see [1]. Synthesis and structure of halocuprates of tetraprotonated 1,4,8,11-tetraazacyclotetradecane and its Cu2+ complex (1989)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 72 (1989), S. 1253-1258 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: By mixing acidic solutions of 1,4,8,11-tetraazacyclotetradecane (Cy) with CuX2 (X = Cl-, Br-), either the hexahalocuprates of the tetraprotonated form of the macrocycle ([CyH4] [CuX6]) or the tetrahalocuprates of its Cu2+ complex ([CuCy] [CuX4]) are obtained. The structures of the chloro derivatives are established by X-ray diffraction analysis. In [CyH4] [CuCl6], the Cu2+ is in a tetragonally distorted octahedral geometry with four short and two long Cu—Cl bonds. The tetraprotonated macrocycle is centrosymmetric, and its conformation is exodentate, so that the four ammonium groups are as far as possible from each other to minimize the electrostatic repulsion. In [CuCy] [CuCl4], the Cu2+ ion complexed by the macrocycle is surrounded by four N-atoms in a square-planar arrangement. In addition, the axial positions are occupied by two Cl- ions of two CuCl42- units, which act as bridges. The macrocycle is in the trans-III-configuration. The other Cu2+ ion is coordinated by four Cl- ions in a distorted tetrahedral geometry. IR and VIS spectra of the chloro and bromo derivatives are used to discuss the structure of the bromo species.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19890720610
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