Publication Date:
1987-02-27
Description:
In initial attempts to define the molecular events responsible for the latent state of herpes simplex virus, in situ hybridization was utilized to search for virally encoded RNA transcripts in latently infected sensory neurons. The use of cloned probes representing the entire viral genome indicated that transcripts encoded within terminal repeats were present. When the alpha genes encoding ICP-0, ICP-4, and ICP-27 and the gamma 1 gene encoding VP-5 were employed, only RNA transcripts hybridizing to the ICP-0 probe were detected. In latently infected cells, the ICP-0--related transcripts were localized principally in the nucleus; this was not the case in acutely (productively) infected neurons or in neurons probed for RNA transcripts coding for actin. In Northern blotting experiments, an RNA of 2.6 kilobases was detected with the ICP-0 probe. When single-stranded DNAs from the ICP-0 region were used as probes, RNA from the strand complementary to that encoding ICP-0 messenger RNA (mRNA) was the major species detected. This RNA species may play a significant role in maintaining the latent infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stevens, J G -- Wagner, E K -- Devi-Rao, G B -- Cook, M L -- Feldman, L T -- AI-06246/AI/NIAID NIH HHS/ -- CA 11861/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1987 Feb 27;235(4792):1056-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2434993" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Ganglia, Spinal/microbiology
;
*Genes, Viral
;
Herpes Simplex/microbiology
;
Mice
;
Neurons/*microbiology
;
Nucleic Acid Hybridization
;
RNA/*genetics
;
RNA, Complementary
;
RNA, Messenger/genetics
;
RNA, Viral/*genetics
;
Simplexvirus/*genetics
;
Transcription, Genetic
;
Viral Proteins/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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