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  • calcium
  • Springer  (5)
  • 2005-2009
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  • 1987  (5)
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  • Springer  (5)
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  • 2005-2009
  • 2000-2004
  • 1985-1989  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 96 (1987), S. 243-249 
    ISSN: 1432-1424
    Keywords: cholera toxin ; ionophore ; calcium ; brush-border membrane vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The physiological relevance of an apparent ionophore activity of cholera toxin towards Ca2+ has been examined in several different systems designed to measure affinity, specificity, rates of ion transfer, and effects on intracellular ion concentrations. Half-maximal transfer rates across porcine jejunal brush-border vesicles were obtained at a concentration of 0.20 μM Ca2+. When examined in the presence of competing ions the transfer process was blocked by very low concentrations of La3+ or Cd2+. Sr2+, Ba2+ and Mg2+ were relatively inefficient competitors for Ca2+ transport mediated by cholera toxin. The relative affinities observed would be compatible with a selectivity for Ca2+ transfer at physiological ion concentrations, as well as an inhibition of this ionophore activity by recognized antagonists of cholera toxin such as lanthanum ions. Entry rates of Ca2+ into brush-border vesicles exposed to cholera toxin were large enough to accelerate the collapse of a Ca2+ gradient generated by endogenous Ca, Mg-ATPase activity. The treatment of isolated jejunal enterocytes with cholera toxin caused a significant elevation in cytosolic Ca2+ concentrations as measured by Quin-2 fluorescence. This effect was specifically prevented by prior exposure of the cholera toxin to excess ganglioside GM1. We conclude that cholera toxin has many of the properties required for promoting transmembranes Ca2+ movement in membrane vesicles and appears to be an effective Ca2+ ionophore in isolated mammalian cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European biophysics journal 14 (1987), S. 441-447 
    ISSN: 1432-1017
    Keywords: Neutron scattering ; lens ; cataract ; calcium ; cold cataract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Small angle neutron scattering (SANS) was used to compare two models of cataracts: the cold cataract induced in the lens nucleus cytoplasm by lowering the temperature and the opacification induced by calcium in the lens cortex cytoplasm. In both cases opacified cytoplasms display additional scattering at low angles as compared to their clear controls. An analysis of this additional scattering provides quantitative information concerning the size distribution, the number and contrast of the scatterers responsible for lens opacification. The scatterers of cold cataract and of calcium—induced opacification not only have, as shown elsewhere, a different composition but are also found to display completely different sizes (in the thousand Å range for cold-cataract, in the hundred Å range for calcium—induced opacification). These results illustrate the diversity of scatterer types which are able to cause comparable lens opacities.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bioscience reports 7 (1987), S. 355-367 
    ISSN: 1573-4935
    Keywords: calcium ; diacylglycerol ; exocytosis ; secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Measurements of intracellular Ca2+ in adrenal medullary cells suggest that a transient rise in Ca2+ leads to a transient secretory response, the rise in Ca2+ being brought about by an influx through voltage-sensitive Ca channels which subsequently inactivate. The level of Ca2+ observed is much smaller than the Ca2+ needed to trigger secretion when introduced directly into the cell. The discrepancy is removed by the presence of diacylglycerot, which increases the sensitivity of the secretory process to Ca2+. The site of action of Ca2+ and diacylglycerol is probably protein kinase C, and tile different secretory responses to increases of Ca2+ and diacylglycerol can be modelled in terms of a preferential order of binding of these two substrates to the enzyme. ATP is needed for secretion: one role is possibly to confer stability to the secretory apparatus; another may involve phosphorylation of some key protein. The kinetics of secretion suggest that if Ca2+ regulates phosphorylation or dephosphorylation, then it is therate of change of phosphorylation that controls secretion rather than theextent of phosphorylation or dephosphorylation. Guanine nucleotide-binding proteins may play a role not only at the level of signal transduction coupling, but also at or near the site of exocytosis, and the mechanism by which some Botulinum toxins inhibit secretion may be associated with these proteins.
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  • 4
    ISSN: 1573-4935
    Keywords: stimulus-secretion coupling ; G-proteins ; mast cells ; calcium ; permeabilised cells ; streptolysin-O ; exocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The secretory process is a coordinated cellular response, initiated by occupation of surface receptors and comprising an ordered sequence of biochemical steps subject to multiple controls. Conceptually we can divide the sequence into two main sections comprising early, receptor-mediated events leading to generation of intracellular second messengers, and later events leading to membrane fusion and exocytosis. With the discovery that occupation of Ca2+ mobilising receptors leads to activation of polyphosphoinositide phosphodiesterase (PPI-pde) through the mediation of a G-protein (Gp), all the early events can be ascribed to the plasma membrane. Investigation of the exocytotic stage of secretion has been simplified by the use of permeabilised cells in which the composition of the cytosol can be precisely controlled. We have used streptolysin-O, a bacterial cytolysin which generates protein-sized pores in the plasma membrane, to investigate the exocytotic mechanism of rat mast cells. We find that in addition to the activation of PPI-dpe, GTP also acts in concert with Ca2+ at, or close to, the exocytotic site. Exocytosis can occur after substantial depletion of cytosol lactate dehydrogenase and 3-phosphoglycerate kinase indicating that soluble cytosol proteins are unlikely to play any role. There is no absolute requirement for ATP or phosphorylating nucleotide in exocytosis though when present the effective affinities of the two obligatory effectors (i.e. Ca2+ and GTP) are substantially enhanced.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 101 (1987), S. 211-221 
    ISSN: 1573-5036
    Keywords: aeration ; calcium ; cotton ; flooding ; magnesium ; manganese ; phosphorus ; potassium ; sodium chloride ; waterlogging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted to investigate the effects of intermittent waterlogging on the nutrient status of cotton (Gossypium hirsutum cv. Deltapine 61). The crop was grown in a sloping plot of soil in which a gradient of water-table depth ranging from 0.04m above to 0.60m below the soil surface was established during two periods of waterlogging in mid summer and early autumn. The first waterlogging lasted 8 days; the second lasted 16 days. Dry matter increases were less for severely waterlogged plants than for plants with well-aerated root systems during the first flooding, but the increases were similar during the second. Waterlogging impaired uptake of most nutrients by young plants in the first flooding, but had much less effect on nutrient uptake by older plants in the second. Waterlogging consistently reduced concentrations of P and K in the petioles and laminae of young fully-expanded leaves, and severely waterlogged plants were deficient in these nutrients by the end of the first flooding. Mn did not accumulate to toxic levels in waterlogged plants. During each flooding, waterlogged plants gained in total content of all nutrients studied, but the gains of each nutrient, except for Na, were proportionally smaller than for well-aerated plants. Fluxes of K-, Cl- and HPO4- ions in xylem sap exuded from stumps of detopped plants which had been waterlogged were lower than those from plants with well-aerated root systems. Seed cotton yields and concentrations of nutrients in mature bolls were not affected by the two periods of waterlogging. It is concluded that although intermittent waterlogging induced nutrient stress in cotton plants, especially for P and K in young plants before flowering, they recovered with no detrimental effect upon yield.
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