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  • Cell & Developmental Biology  (8)
  • ASTROPHYSICS  (6)
  • Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry  (3)
  • Chemical Engineering
  • 1985-1989  (19)
  • 1970-1974
  • 1986  (19)
Collection
Keywords
Publisher
Years
  • 1985-1989  (19)
  • 1970-1974
Year
  • 1
    Electronic Resource
    Electronic Resource
    The use of submicroscopic gold particles combined with video contrast enhancement as a simple molecular probe for the living cell (1986)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 6 (1986), S. 105-113 
    Details
    ISSN: 0886-1544
    Keywords: video-microscopy ; colloidal gold ; immunocytochemistry ; microtubules ; receptors ; saltatory motion ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We describe a new approach to probe the molecular biology of the living cell that uses small colloidal gold particles coupled to specific ligands. They are visualized in cells by bright-field, video enhanced contrast microscopy. We describe the basic aspects of the technique and provide examples of applications to intracellular motility, cell membrane dynamics, receptor translocation, internalization, and intracellular routing. We also provide examples of the use of this approach in immunospecific labelling of cells and tissue sections.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970060207
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  • 2
    Electronic Resource
    Electronic Resource
    Odontogenesis and amelogenesis in interacting lizard - Quail tissue combinations (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 189 (1986), S. 121-129 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this study we examined the possible inductive role of the dental papilla from polyphyodont lizard tooth germs. Flank skin sheets of quail ectoderm enzymatically separated from dermal tissue were recombined with lizard tooth papillae and placed on semisolid medium and cultured for 2 days. Subsequently, the recombinants were removed and placed on the chorioallantoic membrane of chick hosts and incubated for 6 days. After this period of 8 days in explant, control tissues differentiated according to their own phenotypes. Lizard dental papilla alone differentiated as fibroblasts. Quail flank skin ectoderm differentiated into epithelial sheets. Intact lizard tooth buds developed into teeth with dentine and incipient enamel. In the best experimental recombinants, advanced and relatively well-constructed teeth were observed, with clear indications of hard tissue deposition in association with quail epithelium. The results show that mesenchyme of the adult lizard dental papilla and embryonic quail ectoderm of heterotopic origin are capable of carrying out the complex sequence of morphogenetic interactions involved in normal odontogenesis.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051890203
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of embryonic anal processes in Girardinichthys viviparus (Cyprinodontiformes, Osteichthyes) (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 188 (1986), S. 203-224 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Scanning and transmission electron microscopy were used to examine the morphology of the perianal processes (trophotaeniae) of goodeid embryos (Girardinichthys viviparus) at two stages of gestation. The epithelial surface of trophotaeniae is composed of two cell types, one of which shows distinct features associated with absorptive activity. Such cells are characterized by microvilli, abundant mitochondria, and an agranular tubulolamellar network. Micropinocytosis at the apical surface is relatively rare. The brush border membranes contain high levels of alkaline phosphatase. The cells of the second type are the minor component of the trophotaenial epithelium. Their surface is distinct, due to the presence of microridges rather than microvilli. The reticulate arrangement of the cells gives rise to intercellular spaces which occasionally are very large. These interstices are populated with leukocytes. The histological appearance of these sections indicates that this tissue is involved in gas exchange. Embryos at very early stages of development possess similar epithelia which are differentiated to a lesser extent. The connective tissue in some parts of the processes shows structural modifications. It is densely packed with numerous leukocytes occupying the spaces between the cytyoplasmic ramifications of the stroma cells. Possible roles of the trophotaeniae in absorption, respiration, excretion, and the acquisition of immunity are discussed, and it is concluded that the perianal processes of the Goodeidae are more than just trophic embryonic structures.
    Additional Material: 31 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051880207
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence of extruding exocytosis of residual bodies in the freshwater sponge Ephydatia fluviatilis (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 190 (1986), S. 307-318 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Exocytosis of residual bodies by choanocytes, archeocytes and endopinacocytes lining the aquiferous system of Ephydatia fluviatilis has been demonstrated using calibrated latex beads and Escherichia coli as tracers. In passing into the mesohyl or the lumen of the exhalant aquiferous canals, beads, and altered bacteria were enveloped by the plasma membrane of the cell containing them. The membrane constricted at a neck region to form extruding vacuoles. This process appeared first in choanocytes and later in other cell types. The occurrence of these buds increased with the length of incubation time, as did the number of particles they contained. Acid phosphatase activity was frequently associated with the particles budding from the cell membrane, confirming that this process followed digestive activity.Membranous vacuoles were recovered from the external medium and observed by TEM and those adhering to the substratum were seen by SEM. These observations proved that vacuoles were released from the sponges. This membrane-consuming mechanism of exoctyosis implies intense membrane replacement in the digestive cells of the sponge.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051900307
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  • 5
    Electronic Resource
    Electronic Resource
    Cell shape and organization of F-actin and microtubules in randomly moving and stationary amebae of Dictyostelium discoideum (1986)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 6 (1986), S. 176-185 
    Details
    ISSN: 0886-1544
    Keywords: Dictyostelium discoideum ; video and fluorescence microscopy ; random ameboid movement ; stationary mitotic amebae ; cytoskeleton ; microtubule center ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We investigated, by video-light microscopy and fluorescence microscopy with probes specific for microtribules (MTs) and F-actin, the relationship between cytoskeletal elements, cell shape and behavior of vegetative, undifferentiated amebae of Dictyostelium discoideum, strain NC-4. In an unconstrained situation, as on the underside of a coverglass in a thin layer of liquid medium, interphase cells moved around randomly in a polypodial or monopodial fashion. Locomotion was characterized by the formation of pseudodigits, rounded or pointed pseudopodia, and retraction fibers. F-actin occurred in all these structures, as well as in a thin cortical layer. Microtubules extended into some of the cellular extensions rich in F-actin. Pseudopodial activity, but not locomotion, also took place at the interface between medium and air, demonstrating that ameboid movement requires contact with a solid substrate. Stationary mitotic amebae on glass were studded with continuously changing, peripheral spike-shaped filopodia that also contained F-actin. During telophase and cytokinesis the spikes were gradually replaced by pseudopodia in transition to the fully motile phase.In live cells, the nucleus-associated body (NAB), which is at the center of the complex of cytoplasmic MTs [CMTC; term from Brinkley, Fuller, and Highfield, 1975] was in a rather fixed position; it did not orient in a concerted fashion to follow changes in the direction of cell movement. In amebae fixed and processed for fluorescence microscopy after a period of recorded movement, the NAB was not preferentially positioned with respect to the nucleus and the direction of movement. It is unlikely that the NAB exerts a directional control during randon ameboid movement. The complex of cytoskeletal MTs must be very dynamic or flexible to adjust to the rapid changes of cell shape.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970060216
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  • 6
    Electronic Resource
    Electronic Resource
    Electron microscopy of the dorsomedial cortex in the lizard Psammodromus algirus (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 188 (1986), S. 39-49 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cellular populations and the plexiform layers of the dorsomedial cortex of Psammodromus algirus are described at the ultrastructural level. Solitary globous cells are located in the outermost layer of the cortex, the superficial plexiform layer. Double pyramidal cells of the cellular layer show uniform ultrastructural characteristics. Displaced double pyramidal cells, vertical fusiform cells, and globous cells are found in the deep plexiform layer. Two types of dendritic spines are described. Large spines may contain membranous sacs and mitchondira and are located at the upper third of the superifical plexiform layer; small spines do not contain organelles and are located throughout the entire cortex. Two types of axon terminals are widely distributed in both plexiform layers: terminals with only clear vesicles and terminals with both dense-core and clear vesicles. Terminals with large dense-core vesicles may be related to peptidergic synapses and are more frequent at the upper levels of the superficial plexiform layer. The neuroglia described in the dorsomedial cortex of Psammodromus are protoplasmatic astrocytes and oligodendrocytes.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051880105
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  • 7
    Electronic Resource
    Electronic Resource
    Cytoskeletal organization and tissue patterns of epithelia in the sponge Ephydatia mülleri (1986)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 189 (1986), S. 45-65 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Comparative morphological studies on cytoskeletal patterns of sponge basal epithelium at the tissue level have been performed by diverse methods, including immunofluorescence microscopy, and scanning and transmission electron microscopy of stained whole mounts, thin sections or replicas. These methods give results consistent with each other and show the importance of actin assemblies, which function in addition to the microtubular system and in the absence of intermediate filaments. Actin microfilaments indeed are involved in the formation of cables and networks closely associated with the plasma membrane. Both the cables and the networks result from arrangements of microfilaments into bundles of variable size, and the two types of assembly are probably interconvertible. Microfilaments appear to be implicated in the establishment of spot desmosomes and as devices for cell-to-substratum attachment. Due to the desmosomal articulations from cell to cell, the actin cytoskeleton is framed throughout the complete epithelium. It supports the unitary nature of the entire tissue, which is constructed and functions as a whole. It therefore establishes the “histoskeleton” of basic epithelial tissues. The histoskeleton is involved in all epithelial activities but is not uniformly organized into identical cell patterns at the tissue level because activities are sequential and not synchronized in all cells. Similar cytoskeletal patterns exist only in groups of cells, and this suggests that, at a given time, the multiple functions of the epithelium may be mediated by the occurrence of several multicellular functional units within a single epithelial tissue.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051890105
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of cryo-ground rubber on melt flow and mechanical properties of polypropylene (1986)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 26 (1986), S. 1079-1087 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The influence of cryo-ground rubber (CGR) on melt flow and mechanical properties of polypropylene has been investigated. Cryo-ground rubber was incorporated in the polypropylene matrix (a) in powder form and (b) as masterbatch with natural rubber. Natural rubber was found essential as a dispersing agent. The loading of cryo-ground rubber has no significant effect on viscosity at high shear rates (〉61 s-1) but at lower shear rates (〉61 s-1), viscosity functions change shape with increasing cryo-ground rubber content. The dynamic mechanical properties revealed that polypropylene is thermodynamically incompatible with natural rubber and cryo-ground rubber. Cryo-ground rubber in the powder form shows poor adhesion to polypropylene matrix and, therefore, decreases the impact strength at higher loading. Addition of masterbatch of cryo-ground rubber and natural rubber improves the impact strength of polypropylene.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760261508
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  • 9
    Electronic Resource
    Electronic Resource
    Measurement of interfacial tensions of molten polymer systems by means of the spinning drop method (1986)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 26 (1986), S. 415-417 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The spinning drop method is used to measure the interfacial tension of molten polymer systems. It proves to be a simple and rather fast measuring method, while satisfactory agreement with literature values, obtained with the pendant drop method, is achieved.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760260607
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  • 10
    Electronic Resource
    Electronic Resource
    Inhibition of hydrocarbon biosynthesis in the housefly by 2-Octadecynoate (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 3 (1986), S. 75-86 
    Details
    ISSN: 0739-4462
    Keywords: hydrocarbon biosynthesis ; 2-octadecynoate ; housefly ; fatty acid synthetase ; fatty acid elongation ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The elongation of [9,10-3H]oleoyl-CoA with malonyl-CoA to form 20, 22, and 24 carbon monounsaturated fatty acids was demonstrated in housefly microsomes by radio-GLC. These elongation reactions, which have been postulated to be involved in hydrocarbon biosynthesis, have not been previously demonstrated in insects. 2-Octadecynoate (18:1 Δ2=) inhibited the in vivo incorporation of [1-14C]acetate into both fatty acids and hydrocarbons in a dose-dependent manner. At doses of 10 μg per female housefly of the alkynoic acid, the incorporation of [1-14C]acetate into hydrocarbon was inhibited 93%, the incorporation of [9,10-3H]oleate into hydrocarbon was inhibited 64%, and the incorporation of [1-14C]acetate into total internal lipid was inhibited 65%. Partially purified FAS was inhibited 50% and 95% at 15 μM and 40 μM, respectively, of the alkynoic acid. These results show that 2-octadecynoate inhibits hydrocarbon biosynthesis in the housefly by inhibiting FAS, and the in vivo data suggest that the elongation of 18:1 to longer chain fatty acids is also inhibited.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940030109
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