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  • 1
    Publikationsdatum: 2019-10-04
    Beschreibung: Ovarian cancer is the leading cause of death among gynecological cancers, but is poorly amenable to preoperative diagnosis. In this study, we investigate the feasibility of “optical biopsy,” using high-optical-resolution photoacoustic microscopy (OR-PAM) to quantify the microvasculature of ovarian and fallopian tube tissue. The technique is demonstrated using excised human ovary and fallopian tube specimens imaged immediately after surgery. Quantitative parameters are derived using Amira software. The parameters include three-dimensional vascular segment count, total volume and length, which are associated with tumor angiogenesis. Qualitative results of OR-PAM demonstrate that malignant ovarian tissue has larger and more tortuous blood vessels as well as smaller vessels of different sizes, while benign and normal ovarian tissue has smaller vessels of uniform size. Quantitative analysis shows that malignant ovaries have greater tumor vessel volume, length and number of segments, as compared with benign and normal ovaries. The vascular pattern of benign fallopian tube is different than that of benign ovarian tissue. Our initial results demonstrate the potential of OR-PAM as an imaging tool for fast assessment of ovarian tissue and fallopian tube and could avoid unnecessary surgery if the risk of the examined ovary is extremely low.
    Digitale ISSN: 2045-2322
    Thema: Allgemeine Naturwissenschaft
    Publiziert von Springer Nature
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1434-6052
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The production of photons ine + e −→γ+hadrons is investigated at three centre of mass energies around 14, 22 and 34 GeV. On average, photons carry 25% of the total available energy, with a multiplicity similar to the charged multiplicity. The inclusive photon spectra are found to scale with the centre of mass energy as a function of the Feynman variablex. π0 and η mesons are reconstructed from their decay photons. The slopes of the spectra are similar to that for charged pions and approximate scaling is observed for π0 production. The mean π0 and η multiplicities are given. The observed photon yield can be fully accounted for by hadron decays and initial state radiation. However, up to one extra photon per event from other sources cannot be excluded.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 6 (1999), S. 37-42 
    ISSN: 1434-601X
    Schlagwort(e): PACS: 23.20.Lv Gamma transitions and level energies – 27.70.+q 150 〈 A 〈 189
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract: High-spin states in 167Hf, populated in the 141Pr(30Si, p3n)167Hf reaction, have been studied using the nordball Ge detector array. Three rotational cascades have been observed for the first time and the previously-known level scheme has been extended to significantly higher spin. Band-crossing effects are discussed within the framework of Woods-Saxon cranking calculations and are found to be in good agreement.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Environmental geology 30 (1997), S. 72-80 
    ISSN: 1432-0495
    Schlagwort(e): Key words Coal mine workers' pneumoconiosis ; Coalification ; Macerals ; Organic compounds ; Phenols
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie
    Notizen: Abstract  The fibrogenic and cytotoxic potential of coal mine dust is independent of the amount of quartz and other inorganic parameters. Results of coal petrographical and organic geochemical investigations of coals and coal mine dust from the Ruhr and Ibbenbüren Region of Germany demonstrate variations of organic dust amount possibly influencing these noxious properties. Coal mine dust of high rank coals is characterized by a pronounced fibrogenic risk. This risk, independent of the quantity of quartz, is probably based on shape variations of different coal macerals. With increasing coalification of the corresponding seam, the vitrinite is enriched in its dust; however, lower concentrations have been determined for inertinite. Vitrinite shows constant shapes and sizes independent of the rank of coal. Inertinite particles with elongated to fibrous shapes tend to larger sizes with increasing coalification. Strikingly, coal mine dust from miners' lungs with high degrees of coal mine workers' pneumoconiosis (CWP) is enriched in inertinite. In contrast, high cytotoxicities in cell tests are known for coal mine dust from low coalified coals. High concentrations of phenolic compounds can be extracted by dichloromethane from low coalified coal mine dust. These compounds, which are characterized by a high water solubility and therefore high bioavailability, explain the high cytotoxicities of coal mine dust. Contamination of dust by diesel emissions in the coal mine can act as additionally supporting parameters for extended cytotoxicities.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Climate dynamics 14 (1997), S. 17-31 
    ISSN: 1432-0894
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract  The representation of hydrological land surface processes has, so far, been treated inadequately in global models of the atmospheric general circulation (GCMs). In particular the lateral waterflows from the continents into the ocean have been described unsatisfactorily. The aim of this study is to develop a model for the lateral waterflow on the global scale which describes the translation and retention of the lateral discharge as a function of the spatially distributed land surface characteristics that are globally available. Here, global scale refers to the resolution of 0.5° and smaller, corresponding to a typical GCM gridbox area of about 2500 km2. Discharge models need a number of specific input variables that are usually not available from measurements such as runoff and drainage. Therefore, these variables have to be derived from observed data such as precipitation and surface air temperature. For the model development in this study, a new simplified land surface scheme was applied to compute these variables. The discharge from a catchment of approximately the size of a 0.5° gridbox was simulated using several types of models. The intercomparison of the simulated lateral flows of the different models shows that as a minimum a separation between flow processes such as overland flow, baseflow and riverflow is required to yield good discharge simulations. As both the retention and translation of a flow process need to be simulated, a two-parameter model is required for the representation of overland flow and riverflow. For the baseflow, a one-parameter model is sufficient. The resulting model structure is called the hydrological discharge model or HD model. A first parametrization approach was defined using the gridbox characteristics of topography gradient and gridbox length. The skill of the discharge simulation depends not only on the formulations of the model, but also on the precise definition of the boundaries of the model catchments. The sizes and the positioning of the model catchments on the globe were defined by using a river direction file as well as a modified topography dataset. In a first application, the HD model successfully simulated river discharge using runoff and drainage from a five year atmospheric GCM integration (ECHAM4-T42) as input. The annual cycles of the monthly means of the simulated discharge of several large rivers were compared to the observed discharges provided by the Global Runoff Data Centre. The HD model achieves a considerable improvement of the simulated discharge compared to the model which is currently operational at MPI. The improved volume of the discharge is directly related to the definition of more realistic model catchments and the improved timing of the flow is mainly due to the newly introduced separation of the flow processes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Histochemistry and cell biology 83 (1985), S. 531-538 
    ISSN: 1432-119X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The present study describes the intracellular distribution of Tamm-Horsfall protein (THP) in rat kidney. The localization was determined by immunoelectron microscopy using the protein A-gold technique. Various fixation and embedding protocols were evaluated for this purpose. Brief perfusion fixation (3 min) with 1% glutaraldehyde and embedding in a highly hydrophilic glycol methacrylate-polyester mixture were most appropriate for antigen-antibody recognition and structural preservation. The overall tissue distribution of THP was evaluated by indirect immunofluorescence microscopy; reaction was strong along the entire thick ascending limb of the loop of Henle (TAL) with enhanced fluorescence in the apical cytoplasm. On the electron microscopic level immunogold labelling was concentrated over numerous membrane-bound vesicles which form a compartment in the apical cytoplasm. The Golgi region was consistently labelled, whereas the plasma membranes revealed only sporadic labelling at the luminal side, and basolateral membranes were mostly unlabelled. Quantitative evaluation of the gold labelling, which was separately done for the inner stripe, outer stripe and cortical TAL, consistently showed the highest particle density in the apical cytoplasm. Middle and basal levels in the TAL cells were only moderately labelled. The results are discussed with respect to the current opinion which describes THP as a membrane glycoprotein. We speculate that the accumulation of THP in the apical vesicular compartment of TAL cells indicates a storage site of the protein, possibly prior to extrusion via exocytosis of the vesicle contents.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1432-2048
    Schlagwort(e): Key words:Commelina (H+-ATPase) ; C-terminal autoinhibitory domain ; Fusicoccin ; H+-ATPase ; 14-3-3 protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract. The plant plasma-membrane H+-ATPase (EC 3.6.1.35) contains a C-terminal autoinhibitory domain whose displacement from the catalytic site is caused by treatment of intact plant tissue with the phytotoxin fusicoccin (FC). The FC-induced activation of the H+-ATPase was proposed to involve a direct interaction of 14-3-3 proteins with the H+-ATPase. By analysing plasma membranes derived from leaves of Commelina communis L., direct biochemical evidence has now been obtained for a complex between the C-terminus of the H+-ATPase and a 14-3-3 dimer. Stabilization of this complex was achieved by FC treatment in vivo or in vitro. Furthermore, the C-terminal domain of the H+-ATPase in association with a 14-3-3 dimer is essential for the creation of a functional FC-binding complex.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1432-072X
    Schlagwort(e): Key words Chaperone ; Cyanobacteria ; groEL ; isiA ; RNA helicase ; Salt stress ; Subtractive RNA ¶hybridization ; Synechocystis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To identify genes transcribed preferentially under salt stress, a subtractive RNA hybridization procedure was applied to the cyanobacterium Synechocystis sp. PCC 6803. The screening of a genomic library led to the identification of several RNA species that were more abundant in salt-stressed cells than in control cells. Salt-dependent transcription of the identified genes was verified in Northern blot experiments. In addition to the previously characterized genes cpn60 (encoding GroEL; a molecular chaperone) and isiA (encoding a chlorophyll-binding protein), genes encoding a protein of unknown function (slr0082) and a putative RNA helicase (slr0083) were identified as salt-regulated genes in Synechocystis. Genes slr0082 and slr0083, located at sites adjacent to each other on the Synechocystis chromosome, were transcribed from separate promoters and showed the most significant induction 1–3 h after salt shock. The salt-regulated promoters of these two genes were mapped. Genes cpn60, slr0082, and slr0083 were also found to be induced by a cold shock. The possible role of the identified gene products for salt adaptation of Synechocystis is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1432-072X
    Schlagwort(e): Key words Cyanobacteria ; Glucosylglycerol-phosphate ; synthase/phosphatase ; Ions ; Osmolytes ; Salt activation ; Synechocystis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The response of cyanobacteria to a changing osmotic environment includes the accumulation of organic osmolytes such as glucosylglycerol. The activation of the enzymes involved in glucosylglycerol synthesis [glucosylglycerol-phosphate synthase (GGPS) and glucosylglycerol-phosphate phosphatase (GGPP)] in Synechocystis sp. strain PCC 6803 by various salts and salt concentrations was investigated in vitro. GGPS seemed to be the target for salt-mediated regulation of glucosylglycerol synthesis in vitro. GGPS activation was dependent on the concentration of NaCl, and a sigmoidal plot was obtained. Sensitivity to NaCl was markedly enhanced by low Mg+2 concentrations (optimal at 4 mM), but Mg2+ was not absolutely necessary for the Na+ stimulation. As in the case of NaCl, other salts (including MgCl2) stimulated GGPS. The relative order of GGPS activation in the presence of chloride by the cations at constant ionic strength was Li+ 〉 Na+ 〉 K+, Mg2+ Mn2+. No absolute dependence on ionic strength was observed in Mg2+/Na+-exchange experiments. The degree of activation by ions at various concentrations was positively related to the increasing destabilizing properties of the cations according to the Hofmeister rule, where chaotropic cations are most efficient. Cations were responsible for activation since chaotropic anions counteracted the activating effect of cations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 171 (1999), S. 214-217 
    ISSN: 1432-072X
    Schlagwort(e): Key words Cyanobacteria ; Periplasmic proteins ; Salt ; adaptation ; Synechocystis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Periplasmic proteins were obtained from control cells and salt-adapted cells of the cyanobacterium Synechocystis sp. PCC 6803 using the method of cold osmotic shock. Two of these proteins (PP 1, apparent mol. mass 27.6 kDa, and PP 3, apparent mol. mass 39.9 kDa) were accumulated in high amounts in the periplasm of salt-adapted cells, while the major periplasmic protein (PP 2, apparent mol. mass 36.0 kDa) was accumulated independently from salt. After isolation from gels and partial sequencing, the proteins could be assigned to proteins deduced from the complete genome sequence of Synechocystis. Neither salt-induced periplasmic proteins (PP 1, Slr0924 and PP 3, Slr1485) exhibited sequence similarity to proteins of known function from databases. The major protein (PP 2-Slr0513) showed significant sequence similarities to iron-binding proteins. All proteins included typical leader sequences at their N-terminus.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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