Publikationsdatum:
1982-01-08
Beschreibung:
Cultured human lymphocytes and rat hepatoma cells were labeled with [32P]orthophosphate and the insulin receptor subunits identified by immunoprecipitation and sodium dodecyl sulfate-gel electrophoreses. In both cell types the 95,000-dalton (beta) subunit of the insulin receptor was selectively phosphorylated. Phosphorylation was specifically stimulated by insulin in a dose-dependent fashion after 1 and 15 minutes of hormone treatment, whereas human growth hormone was without effect. This phosphorylation may be a very early event in insulin action.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kasuga, M -- Karlsson, F A -- Kahn, C R -- New York, N.Y. -- Science. 1982 Jan 8;215(4529):185-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7031900" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Cells, Cultured
;
Growth Hormone/pharmacology
;
Humans
;
Insulin/*pharmacology
;
Liver Neoplasms, Experimental/metabolism
;
Lymphocytes
;
Macromolecular Substances
;
Molecular Weight
;
Phosphorylation
;
Rats
;
Receptor, Insulin/*metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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