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  • Ultrastructure  (12)
  • Collagen  (4)
  • Springer  (16)
  • 2005-2009
  • 1980-1984  (16)
  • 1955-1959
  • 1980  (16)
Collection
Keywords
Publisher
  • Springer  (16)
Years
  • 2005-2009
  • 1980-1984  (16)
  • 1955-1959
Year
  • 1
    Electronic Resource
    Electronic Resource
    Studies of the intercellular matrix of growth plates from dwarf and homozygous nonaffected alaskan malamutes: Collagen and hexosamine (1980)
    Springer
    Calcified tissue international 32 (1980), S. 237-245 
    Details
    ISSN: 1432-0827
    Keywords: Growth plate ; Dwarf ; Collagen ; Hexosamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary This study was performed to compare the extractability of dwarf growth plate collagen and hexosamine with that of homozygous nonaffected Malamutes and to measure the activity of three of the enzymes involved in the post-translational modifications of the collagen molecule. No significant differences were found in the activity of prolyl hydroxylase or lysyl oxidase in the dwarf growth plates. Lysyl hydroxylase activity in the dwarf was decreased to 22% and 33% that of the activity present in the homozygous nonaffected growth plates. Amino acid analysis of the collagen isolated from dwarf growth plates failed to reveal any decrease in hydroxylysine content. Growth plates were extracted with either 1 M sodium chloride or 4 M guanidine hydrochloride. The extracts were applied to a DEAE-cellulose column. Amino acid analyses of the material which did not bind to DEAE revealed a slight decrease in the amount of guanidine-extractable hydroxyproline in the dwarf but a 60-fold increase in the amount of salt-extractable hydroxyproline in the dwarf growth plates. Material which eluted with 1 M sodium chloride was analyzed for hexosamine. There was a 10-fold increase in the amount of salt-extractable hexosamine present in the dwarf growth plates, whereas no significant differences were observed in the guanidine-extracted material. Hexosamine analysis of the growth plates revealed a significant increase in the total amount of hexosamine present in the dwarf growth plates. SDS-polyacrylamide gels of the material which did not bind to DEAE as well as the pepsin digested, 0.9M sodium chloride precipitated collagen demonstrated the presence of only type II collagen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408547
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure of the prothoracic gland of variously aged female pupae of Xyleborus ferrugineus and associated ecdysteroid titers (1980)
    Springer
    Cell & tissue research 213 (1980), S. 1-8 
    Details
    ISSN: 1432-0878
    Keywords: Ecdysteroids ; Moulting hormones ; Xyleborus ferrugineus pupae ; Ultrastructure ; Prothoracic gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The prothoracic glands of female pupae of Xyleborus ferrugineus at three ages (0-h, 48-h and 72-h-old) were examined for ultrastructural changes that correlate with high titers of ecdysteroids in the entire pupa. In all three ages, the prothoracic gland cells appear compact with a prominent nucleus. Lysosome-like structures with concentrically oriented internal membrane are observed in both 0-h and 72-h-old gland cells. Mitochondria are abundant in the glands of both ages, and rough endoplasmic reticulum is relatively sparse. Numerous microtubules are present in the prothoracic gland cells of 48-h-old female pupae that contain relatively high titers of ecdysteroids. Rough endoplasmic reticulum also is especially abundant at this age of the pupa. Ecdysone titers, estimated by the radioimmunoassay method (RIA), were 161.04±22.61 pg/mg, 704.25±69.02 pg/mg and 298.72±26.80pg/mg body weight in 0-h, 48-h and 72-h-old female pupae, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236915
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  • 3
    Electronic Resource
    Electronic Resource
    The posterior median eyes of the dinopid spider Menneus (1980)
    Springer
    Cell & tissue research 211 (1980), S. 391-403 
    Details
    ISSN: 1432-0878
    Keywords: Ocelli ; Dinopid spider ; Ultrastructure ; Optics ; Evolution of nocturnal eye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The posterior median (pm) eyes of the dinopid spider Menneus unifasciatus L. Koch are described and compared with the pm eyes of Dinopis, which are highly specialised for night vision. The lenses of Menneus have F-numbers of 0.72 compared to 0.58 in Dinopis, the distance between receptors is ca. 4.0 μm compared to 20–22 μm for Dinopis, and image quality is matched to receptor spacing. The lens of Menneus is simple, while that of Dinopis comprises two components of different refractive indices (Blest and Land 1977). Receptive segments of the pm eyes of Dinopis are hexagonal in transverse section and those of adjacent cells are tightly contiguous, allowing the possibility of both optical and electrical coupling (Blest 1978). Receptive segments of Menneus are separated from each other by glial processes containing little pigment, and each segment possesses two rhabdomeres on opposite faces of the cell. Rhabdomere volumes undergo a daily cycle similar to that described for Dinopis, but of relatively minor extent. It is shown that the pm eye of Dinopis could have evolved from that of Menneus by a simple series of transformations, and that a gain of two logarithmic units of sensitivity can be attributed to changes in optical design alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234395
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  • 4
    Electronic Resource
    Electronic Resource
    Piezoelectricity in collagen films (1980)
    Springer
    Calcified tissue international 31 (1980), S. 257-259 
    Details
    ISSN: 1432-0827
    Keywords: Piezoelectricity ; Collagen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Tissue collagen exhibits several levels of structural organization, and this complicates efforts to determine the origin of its piezoelectricity. We made collagen films—by evaporation and electrodeposition from solution—and examined the relation between collagen's piezoelectricity and its electron microscopic appearance. We found that the electrodeposited films were more organized and exhibited higher piezoelectric coefficients than the evaporated films. Despite this, the evaporated films were piezoelectric, thereby suggesting that the effect originates either at the level of the tropocollagen molecule or, at most, with aggregated structures no larger than 50 Å in diameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407190
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  • 5
    Electronic Resource
    Electronic Resource
    Binding of the bone-seeking agent99mTc-1-hydroxyethylidene-1,1-diphosphonic acid to cartilage and collagen in vitro and its stimulation by Er3+ and low pH (1980)
    Springer
    Calcified tissue international 32 (1980), S. 91-94 
    Details
    ISSN: 1432-0827
    Keywords: Diphosphonates ; Cartilage ; Collagen ; Erbium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The bone-seeking agent99mTc-labeled 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) unexpectedly binds to particles of human articular cartilage as well as cortical bone in vitro. Collagen also sequesters this compound, suggesting that collagen contributes to the uptake of99mTc-HEDP by cartilage and bone. Particles of the bone mineral calcium hydroxyapatite also bind99mTc-HEDP in vitro. Pretreatment of particles with Er3+ stimulates binding in each case. Lowering the pH of incubation to pH 2 has this effect for bone, cartilage, and collagen, but not for calcium hydroxyapatite. Mechanisms additional to the simple ionic attraction between the phosphonate groups of HEDP and metal cations such as Ca2+ are responsible for the uptake of99mTc-HEDP by body tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408527
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of calcium in the mandibular condylar growth cartilage of the rat (1980)
    Springer
    Calcified tissue international 30 (1980), S. 27-34 
    Details
    ISSN: 1432-0827
    Keywords: Ultrastructure ; Calcium ; Cartilage ; Vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The potassium pyroantimonate technique was utilized for the selective subcellular localization of calcium in the mandibular condylar cartilage of 1-day-old rats. Electron dense calcium pyroantimonate precipitates were localized principally in mitochondria and at the cell membrane of the chondrocytes. In addition, small intracellular vesicles 0.1–0.2µm in diameter were observed in proximity to the cell membrane of chondrocytes of the mid-hypertrophic zone. The results suggest that these vesicles were being extruded from the cell into the extracellular matrix. Energy-dispersive analysis by X-rays confirmed that calcium is the principal cation of the electron-dense precipitates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408603
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural and gas-chromatographic analysis of the preputial glands of male nude (nu/nu) mice (1980)
    Springer
    Cell & tissue research 211 (1980), S. 73-82 
    Details
    ISSN: 1432-0878
    Keywords: Preputial glands ; Nude mice ; Ultrastructure ; Gas chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The preputial glands of male nude (nu/nu) mice were analyzed by a combination of electron microscopy and gas chromatography to determine whether or not they are affected, like developing hairs and nails, by the nu/nu genotype. Results of the analyses revealed no differences between the glands of nude and normal male mice in either their ultrastructural characteristics or lipid secretory products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233724
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations associated with the initiation of follicular atresia (1980)
    Springer
    Cell & tissue research 211 (1980), S. 105-115 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Preovulatory follicle ; Ultrastructure ; Degeneration ; Atresia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), degenerative changes were observed in focal areas of the granulosa cell layer. These changes include “blebbing” of the cytoplasm and alterations in the shape of the granulosa cells. The appearance of these degenerative changes coincides with a decrease in ovarian concentrations of estradiol and testosterone. Since estrogens and androgens maintain the follicle, the decline in estradiol and testosterone could be responsible for the further degenerative alterations that lead to complete deterioration of the preovulatory follicle. In Stage I atretic follicles, lysosome-derived autophagic vacuoles develop and macrophages invade both the thecal and granulosa cell layers. The combined actions of the autophagic vacuoles and macrophages could destroy both the granulosa-cell and thecal layers and thereby transform the preovulatory follicle into an ovarian cyst.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233727
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  • 9
    Electronic Resource
    Electronic Resource
    Ultrastructural studies of severed medial giant and other CNS axons in crayfish (1980)
    Springer
    Cell & tissue research 208 (1980), S. 123-133 
    Details
    ISSN: 1432-0878
    Keywords: Lesioned axons ; Ultrastructure ; Crayfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distal stumps of severed medial giant axons (MGAs) and of non-giant axons (NGAs) in the CNS of the crayfish Procambarus clarkii show long-term (5–9 months) survival associated with disorientation of mitochondria and thickening of the glial sheath. However, the morphological responses of the two axonal types differ in that neither the proximal nor the distal stump of severed MGAs ever fills with mitochondria as is observed in some severed NGAs. Furthermore, the adaxonal glial layer never completely encircles portions of MGA axoplasm as occurs in many severed NGAs; in fact, ultrastructural changes in the adaxonal layer around severed MGAs are often difficult to detect. No multiple axonal profiles are ever seen within the glial sheath of the proximal or distal stumps of severed MGAs whereas these structures are easily located within severed NGAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234178
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  • 10
    Electronic Resource
    Electronic Resource
    Studies on the ultrastructure of candidate “cysts” inLeptomonas species of siphonaptera (1980)
    Springer
    Parasitology research 63 (1980), S. 233-239 
    Details
    ISSN: 1432-1955
    Keywords: Siphonaptera ; Leptomonas ; Ultrastructure ; Cysts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ultrastructural studies onLeptomonas ctenocephali ofCtenocephalides canis andLeptomonas species ofOrchopeas h. howardi andPalaeopsylla m. minor have demonstrated the presence of characteristic round and oval parasites with a regularly indented plasma membrane. Such forms were found only as unattached parasites in the lumen of the pylorus, hindgut, and rectum amongst parasites which showed a normal kinetoplastid plasmalemma. The plasmalemma indentations are regular in their distribution around the periphery of the parasite, being separated usually by a fixed number of subpellicular microtubules in each “cyst-like form”, some subpellicular microtubules are displaced from the periphery at the point where the plasmalemma is indented furthest into the cytoplasm. It is suggested that these forms meet the criteria necessary to be characterised as “cyst-like forms” despite the apparent absence of a true cyst wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931985
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