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  • RNA  (4)
  • Lepidoptera
  • healthy volunteers
  • Springer  (7)
  • 2005-2009
  • 1980-1984  (7)
  • 1965-1969
  • 1980  (7)
Collection
Publisher
  • Springer  (7)
Years
  • 2005-2009
  • 1980-1984  (7)
  • 1965-1969
Year
  • 1
    ISSN: 1432-2048
    Keywords: Legumin ; Pisum ; Protien synthesis ; RNA ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence is presented to show that legumin, the major storage protein in Pisum, is synthesised in vitro by the wheat germ and reticulocyte lysate systems, from polyribosomes and mRNA isolated from developing pea seeds. While legumin isolated from mature pea seeds consists of 40,000 and 20,000 MW subunits, the in vitro legumin is synthesised as a 60,000 MW precursor consisting of covalently linked 40,000 and 20,000 MW subunits. The implications of these findings are discussed in relationship to studies with other systems.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 349-354 
    ISSN: 1432-1041
    Keywords: propranolol ; radioreceptor assay ; beta-adrenoceptor antagonists ; lung membranes ; plasma level ; healthy volunteers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary A radioreceptor assay (RRA) recently developed in this laboratory for beta-adrenoceptor antagonists in plasma was evaluated in normal volunteers and compared with a radioimmunoassay (RIA) for propranolol. The RRA depends upon the ability of beta-adrenoceptor antagonists to compete with a radiolabelled ligand for beta-adrenoceptor binding sites on lung membranes. Unlike other assays, it measures biologically active drugs including active metabolites of the parent compound. In volunteers given a single oral dose of (±)-propranolol, considerable differences between the two assay methods were demonstrated. In other experiments this difference was shown to relate to the RIA's sensitivity to the inactive (+)-isomer of propranolol and possibly to inactive metabolites. The facility of the RRA in measuring plasma levels of several other non-selective beta-adrenoceptor antagonists was also demonstrated. By employing (−)-propranolol as the standard in the RRA, all of these drugs can be directly compared with a single and relatively simple assay technique.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 18 (1980), S. 743-753 
    ISSN: 1573-4927
    Keywords: RNase-senitive DNA polymerase activity ; RNA ; DNA ; Neurospora crassa ; differential DNA polymerase activity ; cell fractions and mutants of N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract RNase-sensitive DNA polymerase activity (RSDP) was tested in different cell fractions of Neurospora crassa cell types and its morphological mutants. This RSDP was found localized in the microsomal pellet fraction and absent in the purified nuclear pellets isolated from different N. crassa cell types: conidia, germinated conidia, and mycelia. This enzyme is capable of synthesizing a DNA product only in the presence of all four deoxyribonucleoside-5′-triphosphates and Mg2+. Removal of RNA from the pellet fraction by RNase strongly inhibited the DNA synthesis. The endogenous synthesis of DNA in the microsomal pellet fraction was associated with the formation of an RNA:DNA hybrid as analyzed by Cs2SO4 equilibrium density gradient centrifugation. The DNA product after alkali hydrolysis hybridizes with the RNA isolated from the same pellet fraction, as analyzed by elution from hydroxylapatite column at 60 C. This DNA product did not hybridize with poly(A). A few mutants tested showed this RNase-sensitive DNA polymerase activity.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1455-1457 
    ISSN: 1573-8221
    Keywords: neuron ; glia ; RNA ; visual and motor cortex ; visual deprivation ; photic stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-1561
    Keywords: Sex attractant ; trapping ; redbacked cutworm moth ; Euxoa ochrogaster ; Lepidoptera ; Noctuidae ; (Z)-5- and (Z)-7-dodecenyl acetates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mixtures of (Z)-5- and (Z)-7-dodecenyl acetates in ratios 〉100∶1 are powerful, specific sex attractants for redbacked cutworm male moths. Bioactive lures were produced by admixture of these pure compounds or through syntheses involving catalytic bond shifting. Best field trapping was obtained with 200∶1 (Z)-5∶(Z)-7 ratio, at lure doses (on rubber) of 0.1–2.0 mg, using cone swivel traps with 10- to 13-mm orifices, or Pherocon® 1-CP sticky traps, at a height of 50–90 cm. The pheromone traps were more efficient than large blacklight traps and captured the various morphological forms of the moth in similar ratios.
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  • 6
    ISSN: 1573-1561
    Keywords: Electroantennographic detector ; Euxoa ochrogaster ; Lepidoptera ; identification ; mass chromatograms ; mass spectrometry ; redbacked cutworm ; sex pheromone ; (Z)-5-dodecenyl acetate ; (Z)-7-dodecenyl acetate ; (Z)-9-dodecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pheromone washes from calling female moths of redbacked cutworm,Euxoa ochrogaster (Guenée), contained the following acetates that are structurally similar to those of known lepidopteran pheromones (%): decanyl (8.7), dodecanyl (8.5), (E)-5-dodecenyl (3.3), (Z)-5-dodecenyl (76.4), (Z)-7-dodecenyl (3.1), and (Z)-9-dodecenyl (trace〈0.5%). This is the first time that (Z)-5-dodecenyl acetate has been identified as a pheromone component. Three types of specific receptor cells were found in the male antennae, and they responded to (Z)-5-decenyl acetate, (Z)-5- and (Z)-7-dodecenyl acetates, respectively. Strong electroantennographic detector responses were also recorded for these three acetates and for (Z)-5-undecenyl acetate. The evidence for the presence of (Z)-5-decenyl acetate in the pheromone washes was inconclusive. The presence of (Z)-7- and the absence of (Z)-8-dodecenyl acetates were confirmed by a special electroantennographic detector technique in which the detector antennae were from males of other species that were known to have strong responses to these acetates. This is a very useful technique. Field results show that low concentrations (0.1–1.3%) of (Z)-5-decenyl acetate were synergistic when tested in a previously reported blend, but 6% was inhibitory. Similarly, (Z)-7-dodecenyl acetate at 2% or less may be essential for the attraction of males, but in previous tests at 14% it also was inhibitory. Species-specific attractant blends for redbacked cutworm males are described.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 89 (1980), S. 142-144 
    ISSN: 1573-8221
    Keywords: cycloheximide ; protein synthesis ; RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Injection of cycloheximide into rats was shown not to change the acid and alkaline ribonuclease (RNase) activity in the cytoplasm of liver cells. Meanwhile inhibition of protein synthesis by cycloheximide led to a decrease in RNase activity of microsomes and membrane-bound polysomes. RNase activity of free polysomes in liver cells was considerably increased after administration of cycloheximide.
    Type of Medium: Electronic Resource
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