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  • QTL  (10)
  • RAPD  (9)
  • Coleoptera  (7)
  • Springer  (25)
  • Springer Science + Business Media
  • 2000-2004  (20)
  • 1980-1984  (5)
  • 2000  (20)
  • 1980  (5)
Collection
Keywords
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  • Springer  (25)
  • Springer Science + Business Media
Years
  • 2000-2004  (20)
  • 1980-1984  (5)
Year
  • 1
    Electronic Resource
    Electronic Resource
    A genetic map of Maritime pine based on AFLP, RAPD and protein markers (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 39-48 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pinus pinaster ; AFLP ; RAPD ; Protein ; Linkage map ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  TheAFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species characterized by a large genome size (24 pg/C). A genetic linkage map was constructed for one F1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphic DNA) markers. Markers were scored on megagametophytes (1n) from 200 germinated F2 seedlings. Polymorphism rate, labour time and cost of both AFLP and RAPD techniques were compared. The AFLP technique was found to be twice as fast and three-times less costly per marker than the RAPD technique. Thirteen linkage groups were identified with a LOD score ≥6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins were extracted from needles (2n) of the F2 progeny and revealed by 2-DE (two-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection strategy based on the quantification of protein accumulation. Two framework maps of the same F1 individual are now available. The first map (Plomion et al. 1996) uses RAPD markers and the second map, presented in this study, uses mostly AFLP markers. Although the total genetic length of both maps was almost identical, differences among homologous groups were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050006
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  • 2
    Electronic Resource
    Electronic Resource
    RAPD linkage mapping of the shell thickness locus in oil palm (Elaeis guineensis Jacq.) (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 63-70 
    Details
    ISSN: 1432-2242
    Keywords: Key words Elaeis guineensis ; RAPD ; Pseudo-testcross ; Genetic linkage map ; bulked segregant analysis ; Shell thickness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Shell thickness is an important trait in oil palm breeding programs and is the basis for the classification of the varieties of oil palm into the types dura, tenera and pisifera. This trait seems to be controlled by a single locus, with two alleles (sh + and sh −) showing codominant expression. Two single-tree linkage maps were constructed for a maternal tenera (sh + sh −) palm and for a paternal pisifera (sh − sh −) palm using the pseudo-testcross mapping strategy in combination with RAPD markers through the analysis of an F1 tenera×pisifera progeny. A total of 308 arbitrary primers were screened in a sample of eight F1 plants and 121 markers were detected in a testcross configuration. An average of 1.66 polymorphic marker per selected primer were identified in this cross. At LOD 5.0 (with some few exceptions) and θ=0.25 the maternal tenera map included a total of 48 markers distributed in 12 linkage groups or pairs of markers (449.3 cM) while the paternal pisifera map included 42 markers distributed in 15 linkage groups or pairs of markers (399.7 cM). We used RAPD and bulked segregant analysis (BSA) to identify markers more tightly linked to the sh + locus. A total of 174 new primers not previously used in the linkage analysis were screened using bulks of DNA extracted from plants selected for the contrasting shell-thickness phenotypes. Two RAPD markers (R11–1282 and T19–1046) were identified to be linked on both sides of the sh + locus on linkage group 4. The estimated map distances from sh + to R11–1282 and to T19–1046 were 17.5 cM and 23.9 cM, respectively. The results demonstrate the usefulness of RAPD markers and the pseudo-testcross mapping strategy for developing genetic linkage information, and constitute an important step towards early marker-assisted selection for shell thickness in oil palm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050009
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  • 3
    Electronic Resource
    Electronic Resource
    Inheritance of citrus nematode resistance and its linkage with molecular markers (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1010-1017 
    Details
    ISSN: 1432-2242
    Keywords: Key words Tylenchulus semipenetrans ; Bulked segregant analysis ; Linkage map ; QTL ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Eleven RAPD markers linked to a gene region conferring resistance to citrus nematodes in an intergen-eric backcross family were identified. Two sequence- characterized amplified region markers linked to a citrus tristeza virus resistance gene and one selected resistance gene candidate marker were evaluated for their association with citrus nematode resistance. A nematode-susceptible citrus hybrid, LB6-2 [Clementine mandarin (Citrus reticulata)×Hamlin orange (C. sinensis)], was crossed with the citrus nematode-resistant hybrid Swingle citrumelo (C. paradisi×Poncirus trifoliata) to produce 62 hybrids that were reproduced by rooted cuttings. The plants were grown in a greenhouse and inoculated with nematodes isolated from infected field trees. The hybrids segregated widely for this trait in a continuous distribution, suggesting possible polygenic control of the resistance. Bulked segregant analysis was used to identify markers associated with resistance by bulking DNA samples from individuals at the phenotypic distribution extremes. Linkage relationships were established by the inheritance of the markers in the entire population. A single major gene region that contributes to nematode resistance was identified. The resistance was inherited in this backcross family from the grandparent Poncirus trifoliata as a single dominant gene. QTL analysis revealed that 53.6% of the phenotypic variance was explained by this major gene region. The existence of other resistance-associated loci was suggested by the continuous phenotypic distribution and the fact that some moderately susceptible hybrids possessed the resistance-linked markers. The markers may be useful in citrus rootstock breeding programs if it can be demonstrated that they are valid in other genetic backgrounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051382
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  • 4
    Electronic Resource
    Electronic Resource
    The genetic basis of seed-weight variation: tomato as a model system (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1267-1273 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Domestication ; Evolution ; QTL ; Map-based cloning ; Lycopersicon esculentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The seeds of domesticated plants are normally much larger than those of their wild counterparts. This change in seed weight was most likely in response to the selection pressure for yield, uniform germination and seedling vigor which was exerted by humans during domestication. However, despite the evolutionary and agronomic significance of seed weight, very little is know about the genetic and developmental controls of this trait; and, thus far, none of the genes in this pathway have been isolated from any plant species. QTL mapping experiments conducted in tomato during the past decade have allowed the identification of many seed-weight QTLs and have also revealed that only a few loci are responsible for the majority of the seed-weight changes that accompanied the domestication of tomato. This review presents a consensus map for seed weight QTL identified in previously published reports and in unpublished results from our laboratory. This summary of seed-weight QTL data allows for the identification of the major loci controlling this trait in the genus Lycopersicon. It is hoped that this work will allow the elucidation of this important phenotypic transition that occurred during crop-plant domestication and will also provide the starting point for the cloning of a gene responsible for seed-weight variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051433
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular marker assisted genetic analysis of head shattering in six-rowed barley (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 203-210 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brittle rachis ; Weak rachis ; QTL ; Spike density ; Peduncle curvature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Head shattering in barley (Hordeum vulgare L.) has two forms; brittle rachis and weak rachis. Brittle rachis is not observed in cultivated barley since all cultivars carry non-brittle alleles at one of the two complementary brittle rachis loci (Btr1;Btr2). Weak rachis causes head shattering in barley cultivars and may be confused with brittle rachis. Brittle rachis has been mapped to the chromosome 3 (3H) short arm while map position(s) of the weak rachis is unknown. Two major and a putative minor QTL for head shattering were mapped using the Steptoe × Morex doubled haploid line population. The largest QTL, designated Hst-3, located on the chromosome 3 (3H) centromeric region, is associated with a major yield QTL. The Steptoe Hst-3 region, when transferred into Morex, resulted in a substantial decrease in head shattering. High-resolution mapping of Hst-3 was achieved using isogenic lines. Brittle rachis was mapped with molecular markers and shown to be located in a different position from that of Hst-3. The second major QTL, designated Hst-2 S, is located on chromosome 2 S. This locus is associated with an environmentally sensitive yield QTL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051470
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  • 6
    Electronic Resource
    Electronic Resource
    Linkage mapping and QTL analysis in coconut (Cocos nucifera L.) (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 292-300 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; DNA markers ; Early germination ; ISSR ; ISTR ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Different DNA marker types were used to construct linkage maps in coconut (Cocos nucifera L.; 2n = 32) for the two parents of the cross Malayan Yellow Dwarf (MYD) × Laguna Tall (LAGT). A total of 382 markers was sufficient to generate 16 linkage groups for each parent. The total genome length corresponded to 2226 cM for the LAGT map and 1266 cM for the MYD map with 4–32 markers per linkage group. Common markers allowed the association of 9 linkage groups for the two parents MYD and LAGT. QTL analysis for the trait early germination identified six loci. These QTLs correlate with early flowering and yield, representing characters which are important in coconut breeding. The co-segregation of markers with these QTLs provides the first opportunity for marker-assisted selection in coconut breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051482
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  • 7
    Electronic Resource
    Electronic Resource
    Mapping of QTLs conferring resistance to bacterial leaf streak in rice (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 286-291 
    Details
    ISSN: 1432-2242
    Keywords: Key words Rice ; Bacterial leaf streak ; Mapping ; Bulked segregant analysis ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A large F2 and a RI population were separately derived from a cross between two indica rice varieties, one of which was highly resistant to bacterial leaf streak (BLS) and the other highly susceptible. Following artificial inoculation of the RI population and over 2 years of testing, 11 QTLs were mapped by composite interval mapping (CIM) on six chromosomes. Six of the QTLs were detected in both seasons. Eight of the QTLs were significant following stepwise regression analysis, and of these, 5 with the largest effects were significant in both seasons. The detected QTLs explained 84.6% of the genetic variation in 1997. Bulked segregant analysis (BSA) of the extremes of the F2 population identified 3 QTLs of large effect. The 3 QTLs were dentical to 3 of the 5 largest QTLs detected by CIM. The independent detection of the same QTLs using two methods of analysis in separate mapping populations verifies the existence of the QTLs for BLS and provides markers to ease their introduction into elite varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051481
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  • 8
    Electronic Resource
    Electronic Resource
    Host effect on genetic variation of Marssonina brunnea pathogenic to poplars (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 614-620 
    Details
    ISSN: 1432-2242
    Keywords: Key words Aigeiros ; Leuce ; Marssonina brunnea ; Poplar ; RAPD ; Tacamahaca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A broad collection was made for 42 isolates of Marssonina brunnea affecting poplar trees from three different sections (Leuce, Aigeiros and Tacamahaca) within the same Populus genus in China. Genetic diversity among these isolates was analyzed for morphological traits, cultural features, pathogenicity, hyphal anastomosis and randomly amplified polymorphic DNA markers (RAPDs). No significant difference was found in conidial morphological features, such as size, shape and septum location. Yet, considerable differences occur in other characteristics, which leads to the classification of the 42 isolates into two distinct groups, M. brunnea f.sp. monogermtubi and M. brunnea f.sp. multigermtubi. Isolates of M. brunnea f.sp. monogermtubi, derived from section Leuce, germinate only one germ tube, grow fast, produce dark-reddish conidiosorus clusters on the PDA medium, and are highly pathogenic to Populus tomentosa of section Leuce. By contrast, isolates of M. brunnea f.sp. multigermtubi, derived from sections Aigeiros and Tacamahaca, germinate 1–5 germ tubes, grow slowly, produce yellow-greenish conidiosorus clusters on PDA medium, and are pathogenic to Populus ×euramericana cv I-45 and Populus canadensis of section Aigeiros. DNA amplification using 11 RAPD primers generate 78 polymorphic bands among isolates. Cluster analyses based on RAPD markers broadly support such a classification by phenotypes, but provide a new insight into the possible origins of M. brunnea. It is proposed that the pathogen co-evolves with the poplars of section Leuce and has been subsequently distributed to the poplars of sections Aigeiros and Tacamahaca. An isolate from Populus adenopoda of section Leuce is placed in the third group, which is most likely a transmission type from M. brunnea f.sp. monogermtubi to M. brunnea f.sp. multigermtubi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050081
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  • 9
    Electronic Resource
    Electronic Resource
    Identification and molecular mapping of loci controlling fruit ripening time in tomato (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 249-255 
    Details
    ISSN: 1432-2242
    Keywords: Key words QTL ; Earliness ; CAP ; RAPD ; Lycopersicon esculentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Using RAPD marker analysis, two quantitative trait loci (QTLs) associated with earliness due to reduced fruit-ripening time (days from anthesis to ripening = DTR) were identified and mapped in an F2 population derived from a cross between Lycopersicon esculentum’E6203’ (normal ripening) and Lycopersicon esculentum’Early Cherry’ (early ripening). One QTL, on chromosome 5, was associated with a reduction in both ripening time (5 days) and fruit weight (29.3%) and explained 15.8 and 13% of the total phenotypic variation for DTR and fruit weight, respectively. The other QTL, on chromosome 12, was primarily associated with a reduction only in ripening time (7 days) and explained 12.3% of the total phenotypic variation for DTR. The gene action at this QTL was found to be partially dominant (d/a=0.41). Together, these two QTLs explained 25.1% of the total phenotypic variation for DTR. Additionally, two QTLs associated with fruit weight were identified in the same F2 population and mapped to chromosomes 4 and 6, respectively. Together, these two QTLs explained 30.9% of the total phenotypc variation for fruit weight. For all QTLs, the ’Early Cherry’ alleles caused reductions in both ripening time and fruit weight. The polymorphic band for the most significant RAPD marker (OPAB-06), linked to the reduced ripening time QTL on chromosome 12, was converted to a cleaved amplified polymorphism (CAP) assay for marker-aided selection and further introgression of early ripening time (DTR) into cultivated tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050033
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  • 10
    Electronic Resource
    Electronic Resource
    Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 299-307 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Larix ; Linkage map ; RAPD ; AFLP ; ISSR ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic linkage maps have been increasingly developed for a wide variety of plants, using segregating populations such as F2s or backcrosses between inbred lines. These pedigrees are rarely available in outbred species like forest trees which have long generation times. Thus genetic mapping studies have to use peculiar pedigrees and markers in appropriate configurations. We constructed single-tree genetic linkage maps of European larch (Larix decidua Mill.) and Japanese larch [Larix kaempferi (Lamb.) Carr.] using segregation data from 112 progeny individuals of an hybrid family. A total of 266 markers (114 AFLP, 149 RAPD and 3 ISSR loci) showing a testcross configuration, i.e.heterozygous in one parent and null in the other parent, were grouped at LOD 4.0, θ=0.3. The maternal parent map (L. decidua)consisted of 117 markers partitioned within 17 linkage groups (1152 cM) and the paternal parent map (L. kaempferi) had 125 markers assembled into 21 linkage groups (1206 cM). The map distance covered by markers was determined by adding a 34.7-cM independence distance at the end of each group and unlinked marker. It reached 2537 cM and 2997 cM respectively for European larch and Japanese larch, and represented respectively a 79.6% and 80.8% coverage of the overall genome. A few 3:1 segregating markers were used to identify homologous linkage groups between the European larch and the Japanese larch genetic maps. The PCR-based molecular markers allowed the construction of genetic maps, thus ensuring a good coverage of the larch genome for further QTL detection and mapping studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050039
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