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  • Nicotiana  (7)
  • Pisum
  • Springer  (11)
  • Annual Reviews
  • Springer Science + Business Media
  • 1980-1984  (6)
  • 1975-1979  (5)
  • 1984  (6)
  • 1977  (5)
Collection
Keywords
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  • Springer  (11)
  • Annual Reviews
  • Springer Science + Business Media
Years
  • 1980-1984  (6)
  • 1975-1979  (5)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Nicotiana chloroplast genome (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 185-193 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Chloroplast DNA ; Deletion ; Restriction ; Hot spot ; Inverted repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A physical map containing six restriction sites of the Nicotiana tabacum chloroplast genome, together with the BamHI maps of N. tabacum, N. otophora and N. knightiana, and the SmaI maps of N. acuminata, N. plumbaginifolia, N. langsdorffii, N. otophora, N. tabacum, N. tomentosiformis and N. knightiana was constructed. In Nicotiana chloroplast genomes, the most frequently observed variations are point mutations. Deletions are also detected. Most of the observed changes are confined to one area of the large single copy region, which is designated as the “hot spot”. Based on the evidence obtained from Nicotiana chloroplast genomes, an origin of the inverted repeats in this genus is proposed. We suggest that the inverted repeats represent a vestige of what were once two identical, complete chloroplast genomes joined together in a head-to-head and tail-to-tail fashion, and that deletions generated the current chloroplast genome organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317030
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  • 2
    Electronic Resource
    Electronic Resource
    Nicotiana chloroplast genome (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 333-336 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Chlamydomonas ; Chloroplast DNA ; RuBPCase ; E. coli ; B. subtilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272870
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  • 3
    Electronic Resource
    Electronic Resource
    Transport of exogenous auxin in two-branched dwarf pea seedlings (Pisum sativum L.) (1977)
    Springer
    Planta 136 (1977), S. 91-96 
    Details
    ISSN: 1432-2048
    Keywords: Apical dominance ; Auxin transport ; Cambial development ; Pisum ; Polarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dwarf pea plants bearing two cotyledonary shoots were obtained by removing the epicotyl shortly after germination, and the patterns of distribution of 14C in these plants was investigated following the application of [14C]IAA to the apex of one shoot. Basipetal transport to the root system occurred, but in none of the experiments was 14C ever detected in the unlabelled shoot even after transport periods of up to 48 h. This was true both of plants with two equal growing shoots and of plants in which one shoot had become correlatively inhibited by the other, and in the latter case applied whether the dominant or subordinate shoot was labelled. In contrast, when [14C]IAA was applied to a mature foliage leaf of one shoot transfer of 14C to the other shoot took place, although the amount transported was always low. Transport of 14C from the apex of a subordinate shoot on plants bearing one growing and one inhibited shoot was severely restricted compared with the transport from the dominant shoot apex, and in some individual plants no transport at all was detected. Removal of the dominant shoot apex rapidly restored the capacity of the subordinate shoot to transport apically-applied [14C]IAA, and at the same time led to rapid cambial development and secondary vascular differentiation in the previously inhibited shoot. Applications of 1% unlabelled IAA in lanolin to the decapitated dominant shoot maintained the inhibition of cambial development in the subordinate shoot and its reduced capacity for auxin transport. These results are discussed in relation to the polarity of auxin transport in intact plants and the mechanism of correlative inhibition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387930
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  • 4
    Electronic Resource
    Electronic Resource
    Evidence for carrier-mediated transport of L-leucine into isolated pea (Pisum sativum L.) chloroplasts (1977)
    Springer
    Planta 136 (1977), S. 147-151 
    Details
    ISSN: 1432-2048
    Keywords: Carrier ; mediated transport ; Chloroplast ; Leucine ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The uptake of leucine into isolated, intact, pea chloroplasts was investigated using the silicone oil centrifugation technique. The internal: external ratio of leucine exceeded unity at low external leucine concentrations. Uptake of leucine at different external concentrations showed passive diffusion and carrier-mediated transport components. Competition for uptake was shown between leucine and isoleucine but not between leucine and glycine. Rates of diffusion of leucine were found to be low compared with glycine, however, fast carrier-mediated transport of leucine assumed more importance at physiological concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396190
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  • 5
    Electronic Resource
    Electronic Resource
    The coding site of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 97-105 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin-coding-site ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ferredoxins were isolated and purified from leaves of different species of Nicotiana and Petunia and from spinach leaves. Their spectral properties, degree of homogeneity, and molecular weights were determined. The preparations were further analyzed by polyacrylamide gel electrophoresis of tryptic hydrolysates. This allowed us to distinguish between not only ferredoxins of Nicotiana, Petunia, and spinach, but even ferredoxins of various Nicotiana species. We used the differences in tryptic peptide compositions as phenotypic markers to study the mode of inheritance of chloroplast ferredoxin to see whether the coding site is in the chloroplast or in the nucleus. Analysis of the tryptic peptide composition of ferredoxin from different interspecific hybrids of Nicotiana showed that the characteristics of both parental ferredoxins were present. The results indicate that the primary structure of at least the male ferredoxin is coded for in the nucleus. In some of the hybrids the relative contribution of the male parent appeared to be low, suggesting that the female genome (presumably that part located in the plastome) exerted a dominating influence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387545
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  • 6
    Electronic Resource
    Electronic Resource
    Qualitative and quantitative immunofluorescence studies of chloroplast ferredoxin (1977)
    Springer
    Planta 137 (1977), S. 279-286 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Ferredoxin ; Immunofluorescence ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibodies were raised in rabbits against 2Fe−2S ferredoxin from N. tabacum L. The antibodies showed partial cross-reactivity in the double diffusion test with ferredoxins from Spinacia oleracea L., Petunia inflata Fries., P. axillaris Lam., Phaseolus vulgaris L., Chlamydomonas remhardii Dang. A complete cross-reaction was observed with ferredoxins from five other Nicotiana species, thus with this test it was impossible to discriminate between these ferredoxins. Therefore the following test was performed. Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Rabbit anti-N. tabacum-serum was then pre-incubated with this ferredoxin which resulted in complete abolition of cross-reactivity with free heterologous ferredoxin. However, the serum retained antibody activity against specific antigenic determinants of N. tabacum ferredoxin. When this serum was tested against ferredoxin purified from the hybrid: N. tabacum (♀)xN. glutinosa (♂) it gave a positive reaction. The relative content of maternal N. tabacum ferredoxin in the hybrid was estimated by using a fluorescent derivative of this specific antibody and estimating the cross-reactivity compared with that of artificial mixtures of pure N. tabacum and N. glutinosa ferredoxins. The hybrid contained 50% of maternal ferredoxin. This technique was also applied to ferredoxins of other species of Nicotiana and to the ferredoxin from the hybrid N. clevelandii (♀)xN. glutinosa (♂). We conclude that it provides a good test system for the study of the expression of chloroplast ferredoxin in Nicotiana hybrids in general.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388163
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  • 7
    Electronic Resource
    Electronic Resource
    Cytoplasmic inheritance of chloroplast coupling factor 1 subunits (1977)
    Springer
    Biochemical genetics 15 (1977), S. 1143-1146 
    Details
    ISSN: 1573-4927
    Keywords: coupling factor 1 ; chloroplasts ; maternal inheritance ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An analysis of interspecific hybrids of Nicotiana spp. in which one of the parents was sensitive to tentoxin showed that this sensitivity was transmitted only through the female parent. Since tentoxin acts by selectively binding to the α,β subunit complex of chloroplast coupling factor 1, the gene(s) specifying either one or both of these subunits is located in the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484504
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  • 8
    Electronic Resource
    Electronic Resource
    Pollen irradiation and the transfer of maternal genes in Pisum sativum (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 245-248 
    Details
    ISSN: 1432-2242
    Keywords: Pollen ; Irradiation ; Gene transfer ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pollen of Pisum sativum was exposed to doses of 900 to 6,000 r of X-rays prior to pollinating a multiply marked genotype. The first generation progeny closely resembled that produced with unirradiated pollen. In the second generation, five loci were monitored, and the results showed that irradiation enhanced the proportion of maternal information transmitted to the progeny; the practical implications of the data, as well as the mechanism underlying the effect are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317046
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  • 9
    Electronic Resource
    Electronic Resource
    Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombinant types (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 491-497 
    Details
    ISSN: 1432-2242
    Keywords: Tobacco ; Chloroplast DNA ; Cybrids ; Male sterility ; Tentoxin resistance ; Plastid recombinants ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts of a mutant line of Nicotiana tabacum having a maternally-transmitted chlorophyll deficiency were fused with protoplasts of two alloplasmic-male-sterile Nicotiana lines by the “donor-recipient” technique. In both fusion experiments variegated plantlets were regenerated which were shown to contain cytoplasms of mixed chloroplast nature. This confirms that with the “donor-recipient” method one can obtain mixed cytoplasms of genetically different chloroplasts. We present a convenient system to assay for genetic recombination between chloroplasts by combining use of several cytoplasmic markers: vis. chlorophyll pigmentation, chloroplast DNA restriction patterns, tentoxin resistance and male sterility. Within the limits of the experiment no recombinant types were recovered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264891
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  • 10
    Electronic Resource
    Electronic Resource
    Inheritance of legumin and albumin contents in a cross between round and wrinkled peas (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 101-107 
    Details
    ISSN: 1432-2242
    Keywords: Speed proteins ; Legumin ; Albumin ; Heritability ; Pisum ; Pea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Legumin and albumin are the fractions of pea seed proteins preferred to vicilin because of their high sulfur amino acid contents. The joint inheritance of legumin and albumin contents was studied in a cross between to contrasting lines of peas — one with high legumin and low albumin, and the other with low legumin and medium to high albumin. Single seed determinations were made in the parental, F1; F2 and backcross generations using rocket immunoelectrophoresis. In the non-segregating generations (P1, P2 and F1), legumin and albumin contents were negatively correlated (r=−≅0.50). The estimates of correlation coefficients in the segregating generations (F2, BC1 and BC2) were also about −0.5. However, the two estimates based on the round and on the wrinkled seeds separately in the F2 generation were not significantly different from zero. At least four individual round F2 seeds showed the desired recombination of high legumin with high albumin indicating that the unfavorable correlation can be broken. In this cross legumin content showed predominantly additive genetic variation whereas the dominance variance was the largest component for albumin content. A combined “relative sulfur index”, proposed as a convenient measure for selection, showed a narrow sense heritability of 47%. In general these results support the view that sulfur amino acid content of peas can be improved by breeding, but that the required selection regime must take both legumin and albumin content into account.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252322
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