ISSN:
1432-072X
Schlagwort(e):
Fungal Nuclei
;
Isolation
;
Purification
;
Characterization
;
Dermatophyte
;
Microsporum gypseum
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A method is reported for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum. The yield ranged from greater than 90% in crude preparations to less than 20% in highly purified preparations as judged by the recovery of DNA. Nuclei isolated in this manner appeared intact in electron microscopic observations and possessed chemical compositions, i.e. low protein/DNA and RNA/DNA ratios, which indicated a low degree of cytoplasmic contamination.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00590184
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