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  • SPACE SCIENCES  (401)
  • ENERGY PRODUCTION AND CONVERSION
  • Life and Medical Sciences
  • 1980-1984  (118)
  • 1970-1974  (417)
  • 1960-1964
  • 1940-1944
  • 1935-1939
  • 1984  (118)
  • 1972  (417)
Collection
Keywords
Publisher
Years
  • 1980-1984  (118)
  • 1970-1974  (417)
  • 1960-1964
  • 1940-1944
  • 1935-1939
Year
  • 1
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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  • 2
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    Soil mechanics (1972)
    In:  CASI
    Details
    Publication Date: 2005-11-30
    Description: Preliminary results are presented of an investigation of the physical and mechanical properties of lunar soil on the Descartes slopes, and the Cayley Plains in the vicinity of the LM for Apollo 16. The soil mechanics data were derived form (1) crew commentary and debriefings, (2) television, (3) lunar surface photography, (4) performance data and observations of interactions between soil and lunar roving vehicle, (5) drive-tube and deep drill samples, (6) sample characteristics, and (7) measurements using the SRP. The general characteristics, stratigraphy and variability are described along with the core samples, penetrometer test results, density, porosity and strength.
    Keywords: SPACE SCIENCES
    Type: Apollo 16 Prelim. Sci. Rept.; 29 p
    Format: text
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    NASA TECHNICAL REPORTS
  • 3
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    Third Lunar Science Conference. (1972)
    In:  Other Sources
    Details
    Publication Date: 2011-08-16
    Description: Discussion of the nature and properties of lunar rock as deduced from the examination of Apollo lunar rock samples. The topics include the lunar crust, the Fra Mauro formation, the interior of the moon, lunar chronology, surface processes, and earth-moon environment.
    Keywords: SPACE SCIENCES
    Type: Science; 176; June 2
    Format: text
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    NASA TECHNICAL REPORTS
  • 4
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    Chemical composition of sawdust from lunar rock 12013 and comparison of a Java tektite with the rock. (1972)
    In:  Other Sources
    Details
    Publication Date: 2019-06-27
    Description: Abundances of 11 major and minor elements and 11 trace elements have been determined by instrumental neutron activation analysis of two Apollo 12013 rock fragments, a sample of rock 12013,17 sawdust, and a Java tektite (J2). Although the abundances of major elements in tektite J2 are similar to those of rock 12013, comparison of the minor and trace elements shows that no fragment of sawdust or rock 12013 that has been analyzed to date is chemically similar to tektite glass. Rock sawdust is representative of ?whole rock' composition only if the amount of contamination from the sawing process is known. After appropriate correction for saw wire contamination, analyses of sawdust yield fairly accurate averaged elemental compositions of complex clastic lunar and other rocks.
    Keywords: SPACE SCIENCES
    Type: Science; 175; Jan. 14
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    Elemental abundances in the Havero meteorite. (1972)
    In:  Other Sources
    Details
    Publication Date: 2019-06-27
    Description: Abundances of 15 major, minor, and trace elements have been determined in powders and interior chips derived from the Havero ureilite. The values are in close agreement with mean values for other ureilites, as reported in the literature. The powdered samples exhibited slightly higher abundances of O and Si than the interior chips. It is suggested that this may be due to alteration of the samples during the powdering process.
    Keywords: SPACE SCIENCES
    Type: Meteoritics; 7; Dec. 30
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
    Electronic Resource
    Electronic Resource
    Intercellular bridges in ovaries of the newborn gerbil (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 29-35 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study describes intercellular bridges in the ovaries of neonatal gerbils. Electron microscopy has revealed the presence of true intercellular bridges, connecting oogonia or oocytes, in ovaries of newborn gerbils. The cytoplasm of the intercellular channels is similar to that of the connected cells, with mitochondria, smooth and rough endoplasmic reticulum, and free ribosomes present. Lysosomes are also occasionally present in the intercellular bridges and they may be involved in early waves of oocyte atresia. An electrondense substance, 350-500 Å thick, is located immediately beneath the unit membrane of the intercellular bridges. Accumulation of electron-dense material increases the thickness of the walls of the intercellular bridges, supporting and maintaining the patency of the channels. It is suggested that the intercellular channels probably allow the interchange of nutrients, organelles, and possibly regulatory materials as well.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051800105
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  • 7
    Electronic Resource
    Electronic Resource
    A comparison of spindle concentrations in large and small muscles acting in parallel combinations (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 243-252 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A small short muscle frequently acts across a joint in parallel with a vastly larger and longer muscle; therefore it should play a minimal role in the mechanical control of that joint. This study provides evidence suggesting that the small member of such a “parallel muscle combination” (PMC) may serve an important sensory feedback role. The spindle densities of large and small members of PMCs in man and the dog were determined and compared. Epaxial PMCs controlling canine intervertebral joints were dissected and tissue samples were embedded in paraffin, sectioned transversely to the muscles' long axis and, stained with hematoxylin-periodic acid-Schiff (PAS). Representative tissue sections were projected on to stereological grids and the percentage volume of spindles was determined. Data existing in the literature were used to ascertain spindle densities in human PMCs controlling joints in the cervico-occipital region and the extremities. The spindle density for each muscle in a group of PMCs controlling a particular motion was listed, and the mean spindle densities were determined for both the large and the small members of the group. Student's unpaired t test was used to determine the significance of the differences between mean spindle densities. Linear regression was calculated and the data were plotted graphically.In all PMCs examined, the spindle density of the small muscles was significantly higher than that of their large counterparts. It is therefore proposed that the small muscles of PMCs may function as “kinesiological monitors” generating important proprioceptive feedback to the central nervous system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051800307
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  • 8
    Electronic Resource
    Electronic Resource
    Competitive in vivo proliferation of foetal and adult haematopoietic cells in lethally irradiated mice (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 79 (1972), S. 293-298 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The relative proliferative capacity of haematopoietic cell populations derived from 22-week-old adult bone marrow and 14-18 day foetal liver has been studied in lethally irradiated syngeneic recipients by means of chromosome markers. Although starting at a disadvantage in terms of the number of colony-forming units (stem cells) injected, the foetal liver-derived populations steadily increased their relative numbers in the myeloid and lymphoid tissues over a period of several weeks until a plateau was reached. It is suggested that stem cells in foetal liver have, on average, a higher intrinsic capacity for self-renewal than do those in bone marrow, and that this capacity falls to the adult level within about ten weeks of transfer.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040790214
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  • 9
    Electronic Resource
    Electronic Resource
    Lymphopoiesis in the rat. II. The effect of thymectomySupported by grant IC-18A from the American Cancer Society, grant AI-08642 from the National Institute of Allergy and Infectious Disease and grant 211-12-E(13) from the National Tuberculosis and Respiratory Disease Association, Inc. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 80 (1972), S. 13-21 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Lymphopoiesis with respect to recirculating and non-recirculating small lymphocytes was measured simultaneously in rats thymectomized as adults. Removal of the thymus at four to five weeks of age had a profound inhibitory effect upon the production of recirculating cells, whereas the formation of non-recirculating lymphocytes was only slightly depressed. Thymectomy had approximately the same impact of lymphopoiesis as thymectomy and exposure of the animal to a large dose of whole body X- and γ-irradiation. The latter finding, and the failure of a thoracic duct cell transfusion to augment lymphocyte production, accord with the view that the thymus is the principle intermediate source of recirculating small lymphocytes in the normal, unstimulated animal.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040800103
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  • 10
    Electronic Resource
    Electronic Resource
    Lymphopoiesis in the rat. I. The effect of pool size on lymphocyte productionSupported by grant IC-18A from the American Cancer Society, grant AI-08642 from the National Institute of Allergy and Infectious Disease and grant 211-12-E(13) from the National Tuberculosis and Respiratory Disease Association, Inc. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 80 (1972), S. 1-12 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A method is described for measuring lymphopoiesis that enables the production of recirculating and non-recirculating small lymphocytes to be estimated simultaneously. Using this technique, experiments were undertaken to determine whether the production of recirculating cells is influenced by the number present in the recirculating lymphocyte pool. The results suggest that neither a massive lymphocyte transfusion nor depletion of the pool by whole body irradiation or chronic lymph drainage affect the rate at which recirculating small lymphocytes are generated.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040800102
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