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  • ASTROPHYSICS  (661)
  • Life and Medical Sciences  (376)
  • AERODYNAMICS  (343)
  • INSTRUMENTATION AND PHOTOGRAPHY  (214)
  • ENERGY PRODUCTION AND CONVERSION  (176)
  • 1985-1989  (800)
  • 1980-1984  (958)
  • 1960-1964  (12)
  • 1940-1944
  • 1988  (388)
  • 1987  (412)
  • 1984  (495)
  • 1982  (463)
  • 1962  (12)
Collection
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Years
  • 1985-1989  (800)
  • 1980-1984  (958)
  • 1960-1964  (12)
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    Perturbation of the switch-on of transcriptase activity in intermediate subviral particles from reovirus (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120104
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Plant tubulin genes: Structure and differential expression during development (1987)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 8 (1987), S. 435-460 
    Details
    ISSN: 0192-253X
    Keywords: tubulin genes ; microtubules ; Arabidopsis thaliana ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Microtubules are important components of the cytoskeleton of plant cells and play key roles in plant growth and morphogenesis. Recent molecular studies have begun to elucidate the structure and expression of plant genes coding for the major components of microtubules, α- and β-tubulin. Tubulin amino acid sequences deduced from the DNA sequences of eight higher plant tubulin genes are 79-87% homologous with constitutively expressed mammalian tubulins. The genome of the model plant system Arabidopsis thaliana contains four dispersed α-tubulin sequences and at least seven β-tubulin sequences, only two of which appear to be linked. Of the five A. thaliana genes whose expression has been analyzed, the transcripts of one α-tubulin and one β-tubulin gene are constitutively expressed in roots, leaves, and flowers. A second α-tubulin gene is expressed predominately in flowers; the transcripts of the second and third β-tubulin genes are found predominately in leaves or in roots, respectively.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020080511
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    Paper (German National Licenses)
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  • 4
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    VLBI observations of the nucleus of Centaurus A (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Keywords: ASTROPHYSICS
    Type: Extragalactic radio sources; Aug 03, 1981 - Aug 07, 1981; Albuquerque, NM
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    A search for fragmentation debris near Ursa Major Stream stars (1982)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: Accretion models of planet formation and the early cratering history of the solar system suggest that planet formation is accompanied by a cloud of debris resulting from accumulation and fragmentation. A rough estimate of the infrared luminosities of debris clouds is presented for comparison with measured 10-micron luminosities of young stars. New measurements of 13 F, G, and K main-sequence stars of the Ursa Major Stream, which is thought to be about 270-million years old, place constraints on the amount of debris which could be present near these stars.
    Keywords: ASTROPHYSICS
    Type: Icarus; 50; Apr. 198
    Format: text
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  • 6
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    Toward a descriptive model of solar particles in the heliosphere (1988)
    In:  CASI
    Details
    Publication Date: 2013-08-31
    Description: During a workshop on the interplanetary charged particle environment held in 1987, a descriptive model of solar particles in the heliosphere was assembled. This model includes the fluence, composition, energy spectra, and spatial and temporal variations of solar particles both within and beyong 1 AU. The ability to predict solar particle fluences was also discussed. Suggestions for specific studies designed to improve the basic model were also made.
    Keywords: ASTROPHYSICS
    Type: Interplanetary Particle Environment. Proceedings of a Conference; p 3-13
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 7
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    Tunable diode-laser heterodyne spectrometer for remote observations near 8 microns (1982)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: A diode-laser-based, ultrahigh resolution IR heterodyne spectrometer for laboratory and field use has been developed for operation between 7.5 and 8.5 microns. The local oscillator is a PbSe tunable diode laser kept continuously at operating temperatures of 12-60 K using a closed-cycle cooler. The laser output frequency is controlled and stabilized using a high-precision diode current supply, constant temperature controller, and a shock isolator mounted between the refrigerator cold tip and the diode mount. The system largely employs reflecting optics to minimize losses from internal reflection and absorption and to eliminate chromatic effects. Spectral analysis of the diode-laser output between 0 and 1 GHz reveals excess noise at many diode current settings, which limits the IR spectral regions over which useful heterodyne operation can be achieved. Observations have been made of atmospheric N2O, O3, and CH4 between 1170 and 1200/cm, using both a single-frequency swept IF channel and a 64-channel RF spectral line receiver with a total IF coverage of 1600 MHz.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Applied Optics; 21; Jan. 15
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Comparing tiltmeters for crustal deformation measurement - A preliminary report (1984)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: Attention is given to 1.2 years of data from two Michelson-Gale long fluid tiltmeters, one long center pressure tiltmeter, and a shallow borehole tiltmeter, which have been in operation at Pinon Flat Observatory in Southern California. The most stable tilt record shows a secular rate of 0.28 microrad/a, which may be real. Comparisons undertaken among instruments have identified more and less successful measurement techniques. It is concluded that even the best existing sensors must be improved in order to measure continuous tectonic motions.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Geophysical Research Letters (ISSN 0094-8276); 11; 963-966
    Format: text
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  • 9
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    A simplified GPS C/A reciever front end with low noise performance (1984)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: A redesign of the antenna electronics package for the NBS/GPS C/A receiver has resulted in significantly reduced cost and improved performance. Major improvements include a simplified and more reliable multiplier/mixer, elimination of all twelve piston trimmer tuning capacitors in the original design, elimination of expensive bandpass filters, less expensive antenna and a simplified packaging scheme.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: NASA. Goddard Space Flight Center Proc. of the 16th Ann. Precise Time and Time Interval (PTTI) Appl. and Planning Meeting; p 467-474
    Format: application/pdf
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  • 10
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    Evolution of the ultraviolet coma of comet Austin (1982g.) (1984)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: Ultraviolet spectra of comet Austin (1982) obtained in July 1982 at heliocentric distances ranging from 1.10 to 0.81 AU preperihelion with the IUE reveal that the comet is very similar in chemical composition and appearance to comet Bradfield (1979X). In addition, the derived H2O production rate is found to vary with heliocentric distance as r to the -3.6, similar to the r to the -3.7 behavior found for comet Bradfield. It is pointed out, however, that the limited sample rate precludes the observation of the short-term variations which could be produced by a rotating nonuniform cometary nucleus.
    Keywords: ASTROPHYSICS
    Type: Astronomy and Astrophysics (ISSN 0004-6361); 131; 2 Fe
    Format: text
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