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  • Physics  (198)
  • Life and Medical Sciences  (195)
  • SOLAR PHYSICS  (181)
  • Cell & Developmental Biology  (170)
  • INSTRUMENTATION AND PHOTOGRAPHY  (170)
  • 1980-1984  (730)
  • 1960-1964
  • 1950-1954  (14)
  • 1984  (266)
  • 1982  (237)
  • 1980  (227)
  • 1953  (9)
  • 1950  (5)
  • 1933
  • 1932
Collection
Keywords
Publisher
Years
  • 1980-1984  (730)
  • 1960-1964
  • 1950-1954  (14)
  • 1930-1934  (8)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Perturbation of the switch-on of transcriptase activity in intermediate subviral particles from reovirus (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120104
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  • 2
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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  • 3
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    Application Explorer Mission 2 (AEM 2), Stratospheric Aerosol and Gas Experiment (SAGE) (1980)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: NASA, Washington Upper Atmosphere Res. Program; p 234-235
    Format: text
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    NASA TECHNICAL REPORTS
  • 4
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    Observations of a probable change in the solar radius between 1715 and 1979 (1980)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: A decrease in the solar radius is determined using the technique of Dunham and Dunham (1973), in which timed observations are made just inside the path edges. When the method is applied to the solar eclipses of 1715, 1976, and 1979, the solar radius for 1715 is 0.34 + or - 0.2 arc second larger than the recent values, with no significant change between 1976 and 1979. The duration of totality is examined as a function of distance from the edges of the path. Corrections to the radius of the sun derived from observations of the 1976 and 1979 eclipses by the International Occultation Timing Association are also presented.
    Keywords: SOLAR PHYSICS
    Type: Science; 210; Dec. 12
    Format: text
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    NASA TECHNICAL REPORTS
  • 5
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    Tunable diode-laser heterodyne spectrometer for remote observations near 8 microns (1982)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: A diode-laser-based, ultrahigh resolution IR heterodyne spectrometer for laboratory and field use has been developed for operation between 7.5 and 8.5 microns. The local oscillator is a PbSe tunable diode laser kept continuously at operating temperatures of 12-60 K using a closed-cycle cooler. The laser output frequency is controlled and stabilized using a high-precision diode current supply, constant temperature controller, and a shock isolator mounted between the refrigerator cold tip and the diode mount. The system largely employs reflecting optics to minimize losses from internal reflection and absorption and to eliminate chromatic effects. Spectral analysis of the diode-laser output between 0 and 1 GHz reveals excess noise at many diode current settings, which limits the IR spectral regions over which useful heterodyne operation can be achieved. Observations have been made of atmospheric N2O, O3, and CH4 between 1170 and 1200/cm, using both a single-frequency swept IF channel and a 64-channel RF spectral line receiver with a total IF coverage of 1600 MHz.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Applied Optics; 21; Jan. 15
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Comparing tiltmeters for crustal deformation measurement - A preliminary report (1984)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: Attention is given to 1.2 years of data from two Michelson-Gale long fluid tiltmeters, one long center pressure tiltmeter, and a shallow borehole tiltmeter, which have been in operation at Pinon Flat Observatory in Southern California. The most stable tilt record shows a secular rate of 0.28 microrad/a, which may be real. Comparisons undertaken among instruments have identified more and less successful measurement techniques. It is concluded that even the best existing sensors must be improved in order to measure continuous tectonic motions.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Geophysical Research Letters (ISSN 0094-8276); 11; 963-966
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
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    A simplified GPS C/A reciever front end with low noise performance (1984)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: A redesign of the antenna electronics package for the NBS/GPS C/A receiver has resulted in significantly reduced cost and improved performance. Major improvements include a simplified and more reliable multiplier/mixer, elimination of all twelve piston trimmer tuning capacitors in the original design, elimination of expensive bandpass filters, less expensive antenna and a simplified packaging scheme.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: NASA. Goddard Space Flight Center Proc. of the 16th Ann. Precise Time and Time Interval (PTTI) Appl. and Planning Meeting; p 467-474
    Format: application/pdf
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  • 8
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    Tunable diode laser heterodyne spectrometer for remote observations near 8 microns (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Infrared heterodyne spectroscopy is a passive technique employing coherent optical detection for the study of spectral features in remote sources. It employs optical components such as mirrors and lenses normally associated with incoherent optics, but because of it's coherent nature, it offers the advantages of ultrahigh spectral resolving power, high frequency precision, and diffraction limited field-of-view. Attention is given to the development of an ultrahigh resolution diode laser heterodyne spectrometer for observational and laboratory use. The instrument is designed for operation in the spectral range from 7.5 to 8.5 microns. A PbSe tuneable diode laser (TDL) is employed as local oscillator. A closed-cycle cooler is employed to keep the oscillator at operating temperatures in the range from 12 to 60 K. Attention is given to factors determining the TDL heterodyne sensitivity, the spectrometer design, and a survey of 8 micron observations, SiO could be detected in the sunspot spectrum.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Instrumentation in astronomy IV; Fourth Conference; Mar 08, 1982 - Mar 10, 1982; Tucson, AZ
    Format: text
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  • 9
    Electronic Resource
    Electronic Resource
    Intercellular bridges in ovaries of the newborn gerbil (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 29-35 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study describes intercellular bridges in the ovaries of neonatal gerbils. Electron microscopy has revealed the presence of true intercellular bridges, connecting oogonia or oocytes, in ovaries of newborn gerbils. The cytoplasm of the intercellular channels is similar to that of the connected cells, with mitochondria, smooth and rough endoplasmic reticulum, and free ribosomes present. Lysosomes are also occasionally present in the intercellular bridges and they may be involved in early waves of oocyte atresia. An electrondense substance, 350-500 Å thick, is located immediately beneath the unit membrane of the intercellular bridges. Accumulation of electron-dense material increases the thickness of the walls of the intercellular bridges, supporting and maintaining the patency of the channels. It is suggested that the intercellular channels probably allow the interchange of nutrients, organelles, and possibly regulatory materials as well.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051800105
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  • 10
    Electronic Resource
    Electronic Resource
    A comparison of spindle concentrations in large and small muscles acting in parallel combinations (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 243-252 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A small short muscle frequently acts across a joint in parallel with a vastly larger and longer muscle; therefore it should play a minimal role in the mechanical control of that joint. This study provides evidence suggesting that the small member of such a “parallel muscle combination” (PMC) may serve an important sensory feedback role. The spindle densities of large and small members of PMCs in man and the dog were determined and compared. Epaxial PMCs controlling canine intervertebral joints were dissected and tissue samples were embedded in paraffin, sectioned transversely to the muscles' long axis and, stained with hematoxylin-periodic acid-Schiff (PAS). Representative tissue sections were projected on to stereological grids and the percentage volume of spindles was determined. Data existing in the literature were used to ascertain spindle densities in human PMCs controlling joints in the cervico-occipital region and the extremities. The spindle density for each muscle in a group of PMCs controlling a particular motion was listed, and the mean spindle densities were determined for both the large and the small members of the group. Student's unpaired t test was used to determine the significance of the differences between mean spindle densities. Linear regression was calculated and the data were plotted graphically.In all PMCs examined, the spindle density of the small muscles was significantly higher than that of their large counterparts. It is therefore proposed that the small muscles of PMCs may function as “kinesiological monitors” generating important proprioceptive feedback to the central nervous system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051800307
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