Publikationsdatum:
2016-03-26
Beschreibung:
In eukaryotic cells, the nuclear envelope separates the genomic DNA from the cytoplasmic space and regulates protein trafficking between the two compartments. This barrier is only transiently dissolved during mitosis. Here, we found that it also opened at high frequency in migrating mammalian cells during interphase, which allowed nuclear proteins to leak out and cytoplasmic proteins to leak in. This transient opening was caused by nuclear deformation and was rapidly repaired in an ESCRT (endosomal sorting complexes required for transport)-dependent manner. DNA double-strand breaks coincided with nuclear envelope opening events. As a consequence, survival of cells migrating through confining environments depended on efficient nuclear envelope and DNA repair machineries. Nuclear envelope opening in migrating leukocytes could have potentially important consequences for normal and pathological immune responses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Raab, M -- Gentili, M -- de Belly, H -- Thiam, H R -- Vargas, P -- Jimenez, A J -- Lautenschlaeger, F -- Voituriez, Raphael -- Lennon-Dumenil, A M -- Manel, N -- Piel, M -- New York, N.Y. -- Science. 2016 Apr 15;352(6283):359-62. doi: 10.1126/science.aad7611. Epub 2016 Mar 24.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France. Institut Pierre-Gilles de Gennes, PSL Research University, F-75005 Paris, France. ; Institut Curie, PSL Research University, INSERM, U 932, F-75005 Paris, France. ; Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France. ; Laboratoire de Physique Theorique de la Matiere Condensee, CNRS UMR 7600, Universite Pierre et Marie Curie, Paris, France. Laboratoire Jean Perrin, CNRS UMR 8237, Universite Pierre et Marie Curie, Paris, France. ; Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France. Institut Pierre-Gilles de Gennes, PSL Research University, F-75005 Paris, France. matthieu.piel@curie.fr.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/27013426" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Cell Death
;
*Cell Movement
;
Cytoplasm/metabolism
;
*DNA Breaks, Double-Stranded
;
DNA Repair
;
Endosomal Sorting Complexes Required for Transport/genetics/*metabolism
;
HeLa Cells
;
Humans
;
Immunity/genetics
;
Interphase
;
Leukocytes/immunology/ultrastructure
;
Mice
;
Nuclear Envelope/*ultrastructure
;
Nuclear Proteins/metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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