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  • Articles  (13)
  • Articles: DFG German National Licenses  (13)
  • Brassica juncea  (8)
  • Recombinant DNA  (5)
  • 11
    Electronic Resource
    Electronic Resource
    Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 517-521 
    Details
    ISSN: 1432-2242
    Keywords: Ogu cytoplasmic male sterility ; Brassica juncea ; Chlorosis correction ; Protoplast fusion ; Mitochondrial recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male sterility conferred by ogu cytoplasm of Raphanus sativus has been transferred to Brassica juncea cv ‘RLM 198’ from male-sterile B. napus through repeated backcrossing and selection. The male-sterile B. juncea is, however, highly chlorotic and late. It has low female (seed) fertility and small contorted pods. To rectify these defects, protoplasts of the male sterile were fused with normal ‘RLM 198’ (green, self fertile). Four dark green, completely male-sterile plants were obtained and identified as putative cybrids. All the plants were backcrossed three times with ‘RLM 198’. Mitochondrial and chloroplast DNA analysis of backcross progeny confirmed hybridity of the cytoplasm. The restriction pattern of the chloroplast DNA of progeny plants of three cybrids (Og 1, Og 2, Og 3) was similar to that of the green self-fertile ‘RLM 198’ and indicated that the correction of chlorosis resulted from chloroplast substitution. The chloroplast DNA of the lone progeny plant of the fourth cybrid (Og 10) could not be analyzed because the plant was stunted and had only a few leaves. When total cellular DNA was probed with mitochondrial probes coxI and atpA it was found that the cybrids had recombinant mitochondria. The chlorosis-corrected plants were early flowering and had vastly improved seed fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222982
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    A Moricandia arvensis– based cytoplasmic male sterility and fertility restoration system in Brassica juncea (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 488-492 
    Details
    ISSN: 1432-2242
    Keywords: Key words Cytoplasmic male sterility ; Fertility restoration ; Moricandia arvensis ; Brassica juncea ; Protoplast fusion ; Somatic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A cytoplasmic male-sterility system has been developed in mustard (Brassica juncea) following repeated backcrossings of the somatic hybrid Moricandia arvensis (2n=28, MM)+B. juncea (2n=36, AABB), carrying mitochondria and chloroplasts from M. arvensis, to Brassica juncea. Cytoplasmic male-sterile (CMS) plants are similar to normal B. juncea; however, the leaves exhibit severe chlorosis resulting in delayed flowering. Flowers are normal with slender, non-dehiscent anthers and excellent nectaries. CMS plants show regular meiosis with pollen degeneration occurring during microsporogenesis. Female fertility was normal. Genetic information for fertility restoration was introgressed following the development of a M. arvensis monosomic addition line on CMS B. juncea. The additional chromosome paired allosyndetically with one of the B. juncea bivalents and allowed introgression. The putative restorer plant also exhibited severe chlorosis similar to CMS plants but possessed 89% and 73% pollen and seed fertility, respectively, which subsequently increased to 96% and 87% in the selfed progeny. The progeny of the cross of CMS line with the restorer line MJR-15, segregated into 1 fertile : 1 sterile. The CMS (Moricandia) B. juncea, the restorer (MJR-15), and fertility restored F1 plants possess similar cytoplasmic organellar genomes as revealed by ‘Southern’ analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050921
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    Paper (German National Licenses)
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  • 13
    Electronic Resource
    Electronic Resource
    Recombination between chloroplast genomes of Trachystoma ballii and Brassica juncea following protoplast fusion (1998)
    Springer
    Molecular genetics and genomics 260 (1998), S. 357-361 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsTrachystoma ballii ; Brassica juncea ; Somatic hybrid ; Chloroplast genome recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We document here the presence of a recombinant plastome in a cytoplasmic male sterile (CMS) line of Brassica juncea developed from the somatic hybrid Trachystoma ballii + B. juncea. Restriction endonuclease digestion of the chloroplast (cp) DNA has revealed that the recombinant plastome gives rise to novel fragments in addition to the parent-specific fragments. Analysis of the 16S rRNA region by Southern hybridization shows no variation between B. juncea, T. ballii and the CMS line. The rbcL gene region of the recombinant plastome is identical to that in T. ballii. Analysis with probes for psbA and psbD using single and double DNA digests indicates that the hybridization patterns of the recombinant plastome are identical to those of the parents in digests obtained with some restriction enzymes, while novel bands hybridize to probes in other digests. In the psbA region, a B. juncea-specific PstI site and a T. ballii-specific EcoRI site are found in the recombinant plastome. The psbD region of the recombinant plastome contains a B. juncea-specific HindIII site and T. ballii-specific BamHI and HpaII sites. These results indicate the occurrence of intergenomic recombination between the chloroplasts of T. ballii and B. juncea in the somatic hybrid from which the CMS line was developed. The recombined plastome appears to be a mosaic of fragments specific to both parents and the recombination event has occurred in the single-copy regions. These recombinational events have not caused any imbalance in the recombinant plastome in terms of chloroplast-related functions, which have remained stable over generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050904
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